Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T02:20:06.598Z Has data issue: false hasContentIssue false
  • English
  • Français

The Impact of Substrate Topography on Cell Filopodia Extension and Cell Spreading

Published online by Cambridge University Press:  31 January 2011

Lei Yang ,
David Andrew Stout ,
Amy Liang  and
Thomas J Webster
Lei Yang
Affiliation:
[email protected], Brown University, Division of Engineering, Providence, Rhode Island, United States
David Andrew Stout
Affiliation:
[email protected]@gmail.com, California State University, Long Beach, Dept. of Mechanical and Aerospace Engineering, Long Beach, California, United States
Amy Liang
Affiliation:
[email protected], Brown University, Division of Engineering, Providence, Rhode Island, United States
Thomas J Webster
Affiliation:
[email protected], Brown University, Division of Engineering, Providence, Rhode Island, United States
Get access

Abstract

Recent research has found that cell spreading on materials affects cell functions, including proliferation and differentiation. Also, cell spreading is related to filopodia extension which has been shown to be dependent on substrate topography. To better understand this correlation, live-cell imaging was used here to investigate osteoblast (bone forming cell) filopodia extension and cell spreading on two different kinds of diamond. Nanocrystalline diamond (NCD) and submicron crystalline diamond (SMCD) were fabricated to possess similar surface chemistry but different topographies, consisting of nanoscale spherical grains in NCD and submicron polyhedral grains in SMCD. The filopodia extension and cell expansion results showed that cells on nanoscale topographies had faster filopodia extension and greater expansion area than on submicron topographies. Results indicated that substrate topography has an impact on cell filopodia extension and cell spreading, and NCD promoted filopodia extension and cell expansion better than SMCD.

Keywords

nanostructuremicrostructurecrystalline
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1209: Symposia P/YY – Business and Safety Issues in the Commercialization of Nanotechnology , 2009 , 1209-YY02-03
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Referencesa

1 Balasundaram, G. Nanoma Nanomaterials terials for better orthopedics In: TJ, Webster, editor. Nanotechnology for the Regeneration of Hard and Soft Tissues. Singapore: World Scientific, 2007, p. 53–78.Google Scholar
2 Webster, T. J. and Ejiofor, J. U.. Biomaterials 25, 4731, 4731–4739 (2004).Google Scholar
3 Webster, T. J. Ergun, C. Doremus, R. H., Siegel, R. W. Bizio, R. J Biomed Mater Res 51, 475–479 (2000).Google Scholar
4 Dalby, M. J. Riehle, M. O. Sutherland, D. S. Agheli, H. and Curtis, A.S.G.. Int. Biomaterials 25 5415–5422 (2004)Google Scholar
5 Wilson, C. J. Clegg, R. E. Leavesley, D. I. and Pearcy, M. J.. Tissue Eng 11, 1 1–18 (2005).Google Scholar
6 Yang, L. Sheldon, B. W. and Webster, T. J.. Biomaterials 30, 3458–3465 (2009).Google Scholar
7 Choi, C. H. Hagvall, S. H. Wu, B.M., Dunn, J.C.Y., Beygui, R.E., Kim, C.J.. Biomaterials 28 1672–1679 (2007).Google Scholar
8 Gupton, S. L. and Gertler, F. B.. Science STKE 400, re.5 (2007).Google Scholar
9 Yang, L. Sheldon, B. W. and Webster, T. J.. J Biomed Mater Res A 91A, 548–556 (2009).Google Scholar
10 Mongilner, A. and Rubinstein, B.. Biophysical Journal 89 782–95 (2005).Google Scholar