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High Sensitive Optical Detection of Bio-Chemicals onto a Silicon Oxide Surface Based on Waveguide Mode

Published online by Cambridge University Press:  01 February 2011

Nobuko Fukuda ,
Makoto Fujimaki ,
Koichi Awazu ,
Kaoru Tamada  and
Kiyoshi Yase
Nobuko Fukuda
Affiliation:
[email protected], National Institute of Advanced Industrial Science and Technology, Japan (AIST), Photonics Research Institute, Higashi 1-1-1, Tsukuba 305-8565, Tsukuba, N/A, N/A, Japan, +81-29-861-9222, +81-29-861-6821
Makoto Fujimaki
Affiliation:
[email protected], Japan
Koichi Awazu
Affiliation:
[email protected]
Kaoru Tamada
Affiliation:
[email protected]
Kiyoshi Yase
Affiliation:
[email protected]
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Abstract

This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing. Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.

Keywords

sensorsurface reactionsputtering
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 900: Symposium O – Nanoparticles and Nanostructures in Sensors and Catalysis , 2005 , 0900-O12-39
Copyright
Copyright © Materials Research Society 2006

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