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Genetically-Engineered Protease-Activated Triggers in a Pore-Forming Protein

Published online by Cambridge University Press:  15 February 2011

Barbara Walker ,
Nathan Walsh  and
Hagan Bayley
Barbara Walker
Affiliation:
Worcester Foundation for Experimental Biology 222 Maple Avenue, Shrewsbury, MA 01545
Nathan Walsh
Affiliation:
Worcester Foundation for Experimental Biology 222 Maple Avenue, Shrewsbury, MA 01545
Hagan Bayley
Affiliation:
Worcester Foundation for Experimental Biology 222 Maple Avenue, Shrewsbury, MA 01545
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Abstract

Protease-activated triggers have been introduced Into a pore-forming protein, staphylococcal a-hemolysin (αHL). The hemolysin was remodeled by genetic engineering to form two-chain constructs with redundant polypeptide sequences at the central loop, the Integrity of which Is crucial for efficient pore formation. The new hemolysins are activated when the polypeptide extensions are removed by proteases. By alterating the protease recognition sequence in the loop, selective activation by specified proteases can be obtained. Protease-triggered pore-forming proteins might be used for the selective destruction of cancer cells that bear tumor-associated proteases. When certain two-chain constructs are treated with proteases, a full-length polypeptide chain forms as the result of a protease-mediated transpeptidation reaction. This reaction might be used to produce chimeric hemolysins that are Inaccessible by conventional routes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 330: Symposium S – Biomolecular Materials by Design , 1993 , 209
Copyright
Copyright © Materials Research Society 1994

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References

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