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FITC-Functionalized TiO2 Nanoparticles for Simultaneous Neuron Imaging and in Cell Photocatalysis

Published online by Cambridge University Press:  30 September 2014

Tina Zhang ,
Mary Ann Go ,
Christian Stricker ,
Vincent Daria  and
Antonio Tricoli
Tina Zhang
Affiliation:
Nanotechnology Research Laboratory, Research School of Engineering, College of Engineering and Computer Sciences, The Australian National University, Canberra, Australia
Mary Ann Go
Affiliation:
Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia
Christian Stricker
Affiliation:
Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia
Vincent Daria
Affiliation:
Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, Australia
Antonio Tricoli
Affiliation:
Nanotechnology Research Laboratory, Research School of Engineering, College of Engineering and Computer Sciences, The Australian National University, Canberra, Australia
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Abstract

Crystalline TiO2 nanoparticles were produced by scalable flame spray pyrolysis of organometallic solutions. A protocol is presented for the optimized functionalization of these particles with fluorescein isothiocyanate (FITC), an important biomedical dye via a lysine linker. The pH, stoichiometry and time for lysine reaction were determined for highest dye loading and minimized degree of polylysine formation. Acidic reaction conditions, low lysine concentration and short reaction times were found to meet this aim. The resulting particles were used for imaging single neurons, showing high fluorescence emission and ability for the particles to diffuse into small neuron structures such as dendrites.

Keywords

catalyticcompositeflame synthesis
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1694: Symposium EE – Advances in Inorganic Semiconductor Nanoparticles and Their Applications , 2014 , mrss14-1694-ee02-05
Copyright
Copyright © Materials Research Society 2014 

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References

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