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Electric Field Mediated Deposition of Bioactive Polypeptides on Neural Prosthetic Devices

Published online by Cambridge University Press:  15 February 2011

Christopher J. Buchko ,
Kenneth M. Kozloff ,
Atisa Sioshansi ,
K. Sue O'shea  and
David C. Martin
Christopher J. Buchko
Affiliation:
Department of Materials Science and Engineering
Kenneth M. Kozloff
Affiliation:
Department of Materials Science and Engineering
Atisa Sioshansi
Affiliation:
Department of Materials Science and Engineering
K. Sue O'shea
Affiliation:
Department of Cell Biology
David C. Martin
Affiliation:
Department of Materials Science and Engineering Macromolecular Science and Engineering Center University of Michigan, Ann Arbor, Michigan 48109
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Abstract

We have developed processing schemes for depositing three-dimensionally tailored layers of protein polymers on a variety of solid substrates. One of our goals is to create stable, biocompatible coatings on silicon devices for implantation in the central nervous system. Our research has identified several candidate coatings whose morphologies lie in the biologically significant 0.1 to 100 micrometer length scale. Using electric field mediated deposition, we are able to process polypeptides into biologically-responsive films and coatings. Quantitative analysis of the structural evolution of the coating enables us to fine-tune its morphology by varying the field strength and geometry or solution concentration. The interaction of the coated substrates with neurons and glial cells are examined in vivo and in vitro. Data collected from light optical microscopy, atomic force microscopy, transmission electron microscopy, and scanning electron microscopy provide insight about the relationship between the microstructure of these coatings and their macroscopic properties.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 23
Copyright
Copyright © Materials Research Society 1996

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References

1. Langer, R. and Vacanti, J. P., Science, 260, 920–925 (1993).Google Scholar
2. Material provided by Protein Polymer Technologies, Inc., San Diego, CA. Cappello, J., Crissman, J. W., Polymer Preprints, 31 (1), 193–194 (1990).Google Scholar
3. Anderson, J. P., Cappello, J., and Martin, D. C., Biopolymers, 34 (8), 1049–1058 (1994).Google Scholar
4. Michelson, D., Electrostatic Atomization, (Adam Hilger, Bristol, England, 1990).Google Scholar
5. Larrondo, L. and Manley, R. St. John, J. Poly. Sci.: Poly. Phys. Ed., 19, 909–920 (1981).Google Scholar
6. Doshi, J. and Reneker, D. H., Bull. Am. Phys. Soc., 37 (1), 414 (1992).Google Scholar
7. The Center for Neural Communications Technology at the University of Michigan can be reached at http://www.engin.umich.edu/center/cnct/. Wise, K. D. and Najafi, K., Science, 254, 1335–1341 (1991).Google Scholar