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Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

Published online by Cambridge University Press:  15 February 2011

Daxiang Cui ,
Furong Tian ,
Yong Kong ,
Cengiz S. Ozkan ,
Igor Titushikin  and
Huajian Gao
Daxiang Cui
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Furong Tian
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Yong Kong
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Cengiz S. Ozkan
Affiliation:
Department of Mechanical Engineering, University of California, Riverside, CA 92521-0425, USA
Igor Titushikin
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Huajian Gao
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
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Abstract

Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg2+ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 νg/νl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg2+. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 773: Symposium N – Biomicroelectromechanical Systems (BioMEMS) , 2003 , N2.3
Copyright
Copyright © Materials Research Society 2003

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