Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-27T02:36:56.695Z Has data issue: false hasContentIssue false
  • English
  • Français

Device Structure for enhancement of DNA hybridization kinetics by Electrodeless Dielectrophoresis

Published online by Cambridge University Press:  01 February 2011

Nathan Swami ,
Chia-fu Chou  and
Fernanda Camacho-Alanis
Nathan Swami
Affiliation:
[email protected], University of Virginia, Electrical & Computer Engineering, 351 McCormick Rd., PO Box 400743, Charlottesville, VA, 22904, United States
Chia-fu Chou
Affiliation:
[email protected], Academia Sinica, Institute of Physics, 128, Sec. 2, Academia Rd., Nankang, Taipei, 11529, Taiwan
Fernanda Camacho-Alanis
Affiliation:
[email protected], University of Virginia, Electrical & Computer Engineering, 351 McCormick Rd., PO Box 400743, Charlottesville, VA, 22904, United States
Get access

Abstract

Signal transduction for purposes of biomolecular sensing can be enhanced through miniaturization (nanoscale sensors such as nanowires, cantilevers, etc), but this may impose insurmountable limitations on transport of analyte molecules to the surface. In order to improve biomolecular detection sensitivity, this study aims to develop electrodeless dielectrophoresis (EDEP) methods for the selective transport and trapping of analytes in close proximity to the sensor surface. A device design based on microscale dielectric constrictions of the fluidic channel and nanoscale metal electrode edges was used to locally enhance electric field gradients and trap biomolecules. The resulting electric field focusing effect was enhanced as a result of miniaturization and an instantaneous ten-fold EDEP preconcentration was obtained in high-salt buffers (50 mM NaCl) that enabled an equivalent improvement in DNA hybridization kinetics.

Keywords

biomedicalelectrical propertiesfluidics
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1064: Symposium PP – Quantum-Dot and Nanoparticle Bioconjugates–Tools for Sensing and Biomedical Imaging , 2007 , 1064-PP06-08
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Bishop, J., Chagovetz, A., Blair, S., Nanotechnology, 17, 2442–2448 (2006).Google Scholar
[2] Sheehan, P. E., Whitman, L. J., Nano Letters, 5, 803–807 (2005).Google Scholar
[3] Brolo, A. G., Arctander, E., Gordon, R., Leathem, B., Kavanagh, K. L., Nano Letters, 4, 2015–2018 (2004)..Google Scholar
[4] Yonzon, C. R., Jeoungf, E., Zou, S. L., Schatz, G. C., Mrksich, M., Duyne, R. P. Van, J. Am. Chem. Soc., 126, 12669–12676 (2004).Google Scholar
[5] Rindzevicius, T., Alaverdyan, Y., Dahlin, A., Hook, F., Sutherland, D. S., Kall, M., Nano Letters, 5, 2335–2339 (2005).Google Scholar
[6] Cao, Y. W. C., Jin, R. C., Mirkin, C. A., Science, 297, 1536–1540 (2002).Google Scholar
[7] Chou, C. F., Tegenfeldt, J. O., Bakajin, O., Chan, S. S., Cox, E. C., Darnton, N., Duke, T., Austin, R. H., Biophysical Journal, 83, 2170–2179 (2002).Google Scholar
[8] Swami, N., Chou, C.F., Terbrueggen, R., “Two-potential electrochemical probe for study of DNA immobilization”, Langmuir, 21, 1937–1941 (2005).Google Scholar