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Development of Polymeric Micro/Nanostructures For Gene Delivery

Published online by Cambridge University Press:  01 February 2011

Dania Alyounes ,
Christopher Yengo ,
Tim Doran ,
Qi Lu  and
Kenneth Gonsalves
Dania Alyounes
Affiliation:
[email protected], University of North Carolina at Charlotte, Chemistry, 9201 University City Blvd., Charlotte, NC, 28223, United States
Christopher Yengo
Affiliation:
[email protected], University of North Carolina at Charlotte, Biology, 9201 University City Blvd., Charlotte, NC, 28223, United States
Tim Doran
Affiliation:
[email protected], Carolinas Medical Center, Carolinas Neuromuscular/ALS/MDA Center, 1000 Blythe Blvd., Charlotte, NC, 28203, United States
Qi Lu
Affiliation:
[email protected], Carolinas Medical Center, Carolinas Neuromuscular/ALS/MDA Center, 1000 Blythe Blvd., Charlotte, NC, 28203, United States
Kenneth Gonsalves
Affiliation:
[email protected], University of North Carolina at Charlotte, chemistry, 9201 University City Blvd., charlotte, NC, 28223, United States
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Abstract

Pluronic® polymers were screened for binding affinity to oligoribonucleotides (ORN) using fluorescence techniques. F127 (Mw 12600 g/mole) proved to have the strongest binding affinity. Urethane linkages were introduced into F127 and two other Pluronics, F38 (Mw 4700 g/mole) and F77 (Mw 6600 g/mole) to prepare polymers that were multiples of their respective initial molecular weights. A series of these polyurethane Pluronics were screened for binding affinity. Fluorescence studies showed a relationship between the molecular weight of the PO units and the binding affinity in the structures of the newly synthesized polyPluronics, especially in the case of F77. Light scattering confirmed the binding affinity between the ORN and F77 polymers. Particle size analysis showed an exponential increase until the critical micelle concentration. Other analogs of these polymers also studied for their binding affinity were poly(ester amines) and PINC.

Keywords

polymermolecular weightstrength
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1019: Symposium FF – Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease , 2007 , 1019-FF05-13
Copyright
Copyright © Materials Research Society 2007

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