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Development of Fluorescence-based LIDAR Technology for Biological Sensing

Published online by Cambridge University Press:  01 February 2011

Per Jonsson ,
Fredrik Kullander ,
Mikael Tiihonen ,
Melker Nordstrand ,
Torbjørn Tjænhage ,
Pær Wæsterby ,
Gøran Olofsson  and
Mikael Lindgren
Per Jonsson
Affiliation:
Swedish Defence Research Agency (FOI), Sensor Technology, PO Box 1165, SE-581 11 Linkoping, Sweden
Fredrik Kullander
Affiliation:
Swedish Defence Research Agency (FOI), Sensor Technology, PO Box 1165, SE-581 11 Linkoping, Sweden
Mikael Tiihonen
Affiliation:
Department of Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden
Melker Nordstrand
Affiliation:
Swedish Defence Research Agency (FOI), NBC Defence, SE-901 82 Umeå, Sweden
Torbjørn Tjænhage
Affiliation:
Swedish Defence Research Agency (FOI), NBC Defence, SE-901 82 Umeå, Sweden
Pær Wæsterby
Affiliation:
Swedish Defence Research Agency (FOI), NBC Defence, SE-901 82 Umeå, Sweden
Gøran Olofsson
Affiliation:
Swedish Defence Research Agency (FOI), NBC Defence, SE-901 82 Umeå, Sweden
Mikael Lindgren
Affiliation:
Swedish Defence Research Agency (FOI), Sensor Technology, PO Box 1165, SE-581 11 Linkoping, Sweden Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
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Abstract

ABSTRACT:

Results of our on-going development of biological warfare agents (BWA) detection systems based on spectral detection of ultraviolet (UV) laser induced fluorescence (LIF) are presented. A compact optical parametric oscillator (OPO) with intracavity sum-frequency mixing (SFM) to generate 293 nm UV laser irradiation was developed. The OPO/SFM device was pumped by a diode-pumped Nd:YAG laser (1064 nm), including subsequent second-harmonic generation (SHG) in an external periodically poled KTiOPO4 (PPKTP) crystal. The laser generated 1.8 ns pulses at 100 Hz with an average power of 44 mW at 532 nm. The whole system could be used to deliver approximately 30 μJ laser irradiation per pulse (100 Hz) at 293 nm. The spectral detection part of the system consists of a grating and a photomultiplier tube (PMT) array with 32 channels, which can measure fluorescence spectra in the wavelength band from 250 nm to 800 nm. The detector system was designed along with a trigger laser to enable measurement of fluorescence spectra from an individual aerosol particle of simulants for BWA upon excitation with a single nanosecond laser pulse. We demonstrate the successful detection and spectral characterization of simulants for BWA, i.e., Bacillus atrophaeus (BG), Bacillus thuringiensis (BT), and Ovalbumin (OA).

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 883: Symposium FF – Advanced Devices and Materials for Laser Remote Sensing , 2005 , FF1.6
Copyright
Copyright © Materials Research Society 2005

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