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Creating conjugated hydrogel encapsulated membranes

Published online by Cambridge University Press:  01 February 2011

Tae-Joon Jeon ,
Noah Malmstadt  and
Jacob Schmidt
Tae-Joon Jeon
Affiliation:
UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
Noah Malmstadt
Affiliation:
[email protected], UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
Jacob Schmidt
Affiliation:
[email protected], UCLA, Department of Bioengineering, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA, 90095, United States
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Abstract

Device engineering for ion channel proteins requires developing systems that incorporate mechanically stable, long-lived lipid bilayer membranes. Building on our previous work, we have further increased lipid bilayer longevity through covalent conjugation of lipid molecules in the bilayer to an encapsulating hydrogel. This is accomplished by polymerizing the hydrogel in situ around a gigaohm-seal membrane containing vinyl-modified lipid head groups, forming a conjugated hydrogel encapsulated membrane (cgHEM). Membranes formed in this manner show remarkable stability, maintaining gigaohm-level resistance for over 270 hours, better than an order-of-magnitude improvement over the previous state of the art. They also demonstrate the capacity to support the incorporation and measurement of ion channel proteins at the single-molecule level.

Keywords

self-assemblysensorbiomimetic (assembly)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D09-01
Copyright
Copyright © Materials Research Society 2007

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References

REFERENCES

1. Bayley, H. and Cremer, P.S., Stochastic sensors inspired by biology. Nature, 2001. 413(6852): p. 226–230.Google Scholar
2. Guan, X.Y., et al., Stochastic sensing of TNT with a genetically engineered pore. Chembiochem, 2005. 6(10): p. 1875–1881.Google Scholar
3. Nakane, J.J., Akeson, M., and Marziali, A., Nanopore sensors for nucleic acid analysis. Journal of Physics-Condensed Matter, 2003. 15(32): p. R1365–R1393.Google Scholar
4. Zhang, N., Li, N., and Li, L., Liquid chromatography MALDI MS/MS for membrane proteome analysis. Journal of Proteome Research, 2004. 3(4): p. 719–727.Google Scholar
5. Jeon, T.J., Malmstadt, N., and Schmidt, J.J., Hydrogel-encapsulated lipid membranes. Journal of the American Chemical Society, 2006. 128(1): p. 42–43.Google Scholar
6. Knoll, W., et al., Functional tethered lipid bilayers. Reviews in Molecular Biotechnology, 2000. 74(3): p. 137–158.Google Scholar
7. Rehak, M. and Hall, E.A.H., Examination of bilayer lipid membranes for ‘pin-hole’ character. The Analyst, 2004. 129: p. 1014–1025.Google Scholar
8. Castellana, E.T. and Cremer, P.S., Solid supported lipid bilayers: From biophysical studies to sensor design. Surface Science Reports, 2006. 61(10): p. 429–444.Google Scholar
9. Alvarez, O., How to set up a bilayer system., in Ion Channel Reconstitution, Miller, C., Editor. 1986, Plenum Press: New York. p. 115–139.Google Scholar
10. Hermanson, G.T., Bioconjugate techniques. 1996, San Diego: Academic Press. xxv, 785.Google Scholar
11. Landgrebe, J.A., Theory and practice in the organic laboratory: with microscale and standard scale experiments. 4th ed. 1993, Pacific Grove, Calif.: Brooks/Cole Pub. xxii, 586 p.Google Scholar
12. Jeon, T.J., Malmstadt, N., and Schmidt, J., Mechanical studies of hydrogel encapsulated membranes. Materials Research Society Symposium Proceedings, 2006. 926(0926-CC04-03).Google Scholar
13. Gu, L.Q., et al., Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter. Nature, 1999. 398(6729): p. 686–690.Google Scholar