Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-27T01:52:10.814Z Has data issue: false hasContentIssue false
  • English
  • Français

Control of Cell Adhesion and Detachment on Temperature-Responsive Block Copolymer Langmuir Films

Published online by Cambridge University Press:  28 March 2014

Morito Sakuma ,
Yoshikazu Kumashiro ,
Masamichi Nakayama ,
Nobuyuki Tanaka ,
Umemura Kazuo ,
Masayuki Yamato  and
Teruo Okano
Morito Sakuma
Affiliation:
Department of Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo, 162-8601 JAPAN Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Yoshikazu Kumashiro
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Masamichi Nakayama
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Nobuyuki Tanaka
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Umemura Kazuo
Affiliation:
Department of Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo, 162-8601 JAPAN
Masayuki Yamato
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Teruo Okano
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JAPAN
Get access

Abstract

This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Block copolymer composed of polystyrene (PSt) and PIPAAm was synthesized by RAFT polymerization. PSt-block-PIPAAm (St-IP) with various chemical compositions was dropped on an air-water interface and formed Langmuir film by compression. Then, the Langmuir film changing a density was transferred on a hydrophobic modified glass substrate to produce St-IP transferred surface (St-IP LS surface). From the observation of atomic force microscope images, a nanostructure was observed on the transference of Langmuir films. Cell adhesion and detachment were also evaluated on the LS surfaces in response to temperature. Cell adhesion on LS surfaces at 37 °C was controlled by changing the chemical compositions and densities. After reducing temperature to 20 °C, adhering cells rapidly detached themselves with lower Am and higher composition of PIPAAm. Our method should be proved novel insights for investigating cell adhesion and detachment on thermo-responsive surfaces.

Keywords

biomaterialpolymerfilm
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 101 - 106
Copyright
Copyright © Materials Research Society 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Li, Y. and Tanaka, T., J. Chem. Phys. 92, 1365 (1990).CrossRefGoogle Scholar
Heskinsa, M. and Guilleta, J. E., J. Macro. Sci. 2, 1441 (1968).CrossRefGoogle Scholar
Maeda, Y., Higuchi, T. and Ikeda, I., Langmuir 16, 7503 (2000).CrossRefGoogle Scholar
Yang, J., Yamato, M., Kohno, C., Nishimoto, A., Sekine, H., Fukai, F. and Okano, T., Biomaterials 26, 6415 (2005).CrossRefGoogle Scholar
Yamato, M. and Okano, T., Mater. Today 7, 42 (2004).CrossRefGoogle Scholar
Fukumori, K., Akiyama, Y., Kumashiro, Y., Kobayashi, J., Yamato, M., Sakai, K., and Okano, T., Macromol. Biosci. 10, 1117 (2010).CrossRefGoogle Scholar
Akiyama, Y., Kikuchi, A., Yamato, M. and Okano, T., Langmuir 20, 5506 (2004).CrossRefGoogle Scholar
Nakayama, M., Yamada, N., Kumashiro, Y., Kanazawa, H., Yamato, M. and Okano, T., Macromol. Biosci. 12, 751 (2012).CrossRefGoogle Scholar
Sakuma, M., Kumashiro, Y., Nakayama, M., Tanaka, N., Umemura, K., Yamato, M. and Okano, T., J. Biomater. Sci., Polym. Ed. 25, 431 (2014).CrossRefGoogle Scholar
Nagano, S., Matsushita, Y., Ohnuma, Y., Shinma, S. and Seki, T., Langmuir 22, 5233 (2006).CrossRefGoogle Scholar