Hostname: page-component-745bb68f8f-grxwn Total loading time: 0 Render date: 2025-01-14T02:29:17.724Z Has data issue: false hasContentIssue false
  • English
  • Français

Competitive Adsorption of Lung Surfactant and Serum Proteins at the Air-Liquid Interface: A Grazing Incidence X-Ray Diffraction Study

Published online by Cambridge University Press:  01 February 2011

Patrick C Stenger ,
Guohui H Wu ,
Eva Y Chi ,
Shelli L Frey ,
Ka Yee C Lee ,
Jaroslaw Majewski ,
Kristian Kjaer  and
Joseph A Zasadzinski
Patrick C Stenger
Affiliation:
[email protected], University of California, Santa Barbara, Department of Chemical Engineering, Engineering 2, Room 3357, Santa Barbara, CA, 93106-5080, United States
Guohui H Wu
Affiliation:
[email protected], University of California, Department of Chemical Engineering, Santa Barbara, CA, 93106-5080, United States
Eva Y Chi
Affiliation:
[email protected], the University of Chicago, Chicago, IL, 60637, United States
Shelli L Frey
Affiliation:
[email protected], the University of Chicago, Chicago, IL, 60637, United States
Ka Yee C Lee
Affiliation:
[email protected], the University of Chicago, Chicago, IL, 60637, United States
Jaroslaw Majewski
Affiliation:
[email protected], Los Alamos National Laboratory, Los Alamos, NM, 87545, United States
Kristian Kjaer
Affiliation:
[email protected], Risø National Laboratory, Roskilde, N/A, Denmark
Joseph A Zasadzinski
Affiliation:
[email protected], University of California, Department of Chemical Engineering, Santa Barbara, CA, 93106-5080, United States
Get access

Abstract

The competitive adsorption of lung surfactant (LS) and albumin at the air-liquid interface and the ability of polyethylene glycol (PEG) to mediate LS adsorption are analyzed using pressure area isotherms and grazing incidence x-ray diffraction (GIXD). The addition of albumin drastically reduces the amount of LS on the interface and slightly increases the LS lattice spacing. The addition of PEG restores the characteristic LS peaks, yielding a slightly more compact lattice. The scattering results are consistent with recent work which proposed that albumin creates a physical barrier which eliminates LS adsorption and that PEG enhances LS adsorption but does not significantly change LS surface ordering.

Keywords

x-ray diffraction (XRD)adsorptionbiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1027: Symposium D – Materials in Transition–Insights from Synchrotron and Neutron Sources , 2007 , 1027-D05-09
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Notter, R., Lung surfactant: basic science and clinical applications vol. 149. New York: Marcel Dekker, 2000.10.1201/9781482270426Google Scholar
2. Rubenfeld, G. D., Caldwell, E., Peabody, E., Weaver, J., Martin, D. P., Neff, M., Stern, E. J., and Hudson, L. D., New Engl J Med, 353, 1685–1693, (2005).Google Scholar
3. Ware, L. B. and Matthay, M. A., New Engl J Med, 342, 1334–1349, (2000).10.1056/NEJM200005043421806Google Scholar
4. Warriner, H. E., Ding, J., Waring, A. J., and Zasadzinski, J. A., Biophys. J., 82, 835–842, (2002).10.1016/S0006-3495(02)75445-3Google Scholar
5. Zasadzinski, J. A., Alig, T. F., Alonso, C., Serna, J. B. de la, Perez-Gil, J., and Taeusch, H. W., Biophys. J., 89, 1621–1629, (2005).10.1529/biophysj.105.062646Google Scholar
6. Lu, K. W., Goerke, J., Clements, J. A., and Taeusch, H. W., Pediatr Res, 58, 206–210, (2005).10.1203/01.PDR.0000169981.06266.3EGoogle Scholar
7. Stenger, P. C. and Zasadzinski, J. A., Biophys. J., 92, 3–9, (2007).Google Scholar
8. Lee, K. Y. C., Gopal, A., Nahmen, A. von, Zasadzinski, J. A., Majewski, J., G. S. Smith, Howes, P. B., and Kjaer, K., J Chem Phys, 116, 774–783, (2002).Google Scholar
9. Alonso, C., Alig, T., Yoon, J., Bringezu, F., Warriner, H., and Zasadzinski, J. A., Biophys. J., 87, 4188–4202, (2004).10.1529/biophysj.104.051201Google Scholar
10. Braun, A., Stenger, P. C., Warriner, H. E., Zasadzinski, J. A., Lu, K. W., and Taeusch, H. W., Biophys. J., 93, 123–139, (2007).10.1529/biophysj.106.095513Google Scholar
11. Jensen, T. R. and Kjaer, K., "Structural Properties and Interactions of Thin Films at the Air-Liquid Interface Explored by Synchrotron X-Ray Scattering" in Novel Methods to Study Interfacial Layers. vol. 11, Möbius, D. and Miller, R., Eds.: Elsevier Science, 2001, pp. 205–254.10.1016/S1383-7303(01)80028-4Google Scholar