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Characterization of Biodegradable Polyester Films and their Interaction with Hepatocytes

Published online by Cambridge University Press:  15 February 2011

Ann Park ,
Linda Griffith Cima  and
P.-Matthias Kaufmann
Ann Park
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139
Linda Griffith Cima
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139
P.-Matthias Kaufmann
Affiliation:
Department of Surgery, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115
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Abstract

Polymers derived from lactic and glycolic acid have achieved success in many medical applications and have been investigated as possible materials for degradable scaffolds required in tissue transplantation. Previous investigators have found significant differences in the behavior of cells cultured on chemically similar polyesters of this family but the mechanisms for these differences have remained unclear.

In this study, we fabricated polylactic acid, polylactic-co-glycolic acid, and blend films by melt-processing. The bulk and surface properties of these films were characterized by GPC, DSC, WAXS, SEM, and contact angle. All films were smooth and amorphous and differed only in chemical composition. The films were assessed for their ability to sustain or promote hepatocyte attachment, growth, and function. Cells adhered to and exhibited similar morphology on all substrates. Cells decreased in number to 50–60% of the initial cell number but exhibited high retention of differentiated function on all of the polymer substrates over five days. Cell response was not statistically different on the different substrates indicating that chemical composition, in our range of polymer formulations, does not affect cell behavior.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 159
Copyright
Copyright © Materials Research Society 1994

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