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Characterisation of Cell Adhesion to Substrate Materials and the Resistance to Enzymatic and Mechanical Cell-Removal

Published online by Cambridge University Press:  01 February 2011

Helen Jane Griffiths ,
John G Harvey ,
James Dean ,
James A Curran ,
Athina E Markaki  and
T William Clyne
Helen Jane Griffiths
Affiliation:
[email protected], University of Cambridge, Materials Science and Metallurgy, Pembroke St, Cambridge, CB23QZ, United Kingdom, +44 1223 334340, +44 1223 334567
John G Harvey
Affiliation:
[email protected], University of Cambridge, Department of Materials Science and Metallurgy, Pembroke St, Cambridge, CB2 3QZ, United Kingdom
James Dean
Affiliation:
[email protected], University of Cambridge, Department of Materials Science and Metallurgy, Pembroke St, Cambridge, CB2 3QZ, United Kingdom
James A Curran
Affiliation:
[email protected], University of Cambridge, Department of Materials Science and Metallurgy, Pembroke St, Cambridge, CB2 3QZ, United Kingdom
Athina E Markaki
Affiliation:
[email protected], University of Cambridge, Department of Materials Science and Metallurgy, Pembroke St, Cambridge, CB2 3QZ, United Kingdom
T William Clyne
Affiliation:
[email protected], University of Cambridge, Department of Materials Science and Metallurgy, Pembroke St, Cambridge, CB2 3QZ, United Kingdom
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Abstract

Cell-implant adhesive strength is important for prostheses. In this paper, an investigation is described into the adhesion of bovine chondrocytes to Ti6Al4V-based substrates with different surface roughnesses and compositions. Cells were cultured for 2 or 5 days, to promote adhesion. The ease of cell removal was characterised, using both biochemical (trypsin) and mechanical (accelerated buoyancy and liquid flow) methods. Computational fluid dynamics (CFD) modelling has been used to estimate the shear forces applied to the cells by the liquid flow. A comparison is presented between the ease of cell detachment indicated using these methods, for the three surfaces investigated.

Keywords

adhesionbiomaterialTi
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1097: Symposium GG – Mechanical Behavior of Biological Materials and Biomaterials , 2008 , 1097-GG03-05
Copyright
Copyright © Materials Research Society 2008

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