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Cell Response and Tissue Scaffold Triggers Investigated by Scanning Probe Recognition Microscopy

Published online by Cambridge University Press:  01 February 2011

Qian Chen ,
Y. Fan ,
S. Kumar ,
A. D. Baczewski ,
L. Udpa ,
V. M. Ayres ,
I. Ahmed ,
S. Meiners  and
A. F. Rice
Qian Chen
Affiliation:
[email protected], Michigan State University, Electrical & Computer Engineering, 2712 Trappers Cove APT 2C, lansing, MI, 48910, United States
Y. Fan
Affiliation:
[email protected], Michigan State University, East Lansing, MI, 48824, United States
S. Kumar
Affiliation:
[email protected], Michigan State University, East Lansing, MI, 48824, United States
A. D. Baczewski
Affiliation:
[email protected], Michigan State University, East Lansing, MI, 48824, United States
L. Udpa
Affiliation:
[email protected], Michigan State University, East Lansing, MI, 48824, United States
V. M. Ayres
Affiliation:
[email protected], Michigan State University, East Lansing, MI, 48824, United States
I. Ahmed
Affiliation:
[email protected], UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, 08854, United States
S. Meiners
Affiliation:
[email protected], UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, 08854, United States
A. F. Rice
Affiliation:
[email protected], Veeco Metrology Group, Santa Barbara, CA, 93117, United States
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Abstract

Format

This is a copy of the slides presented at the meeting but not formally written up for the volume.

Abstract

Tissue scaffolds have recently demonstrated widespread application in injury healing due to their biomimetic properties and their structural resemblance to the extracellular matrix in cell re-growth. The investigation of cell response to biomimetic triggers provided by tissue scaffolds will greatly extend the impact of this field.

In this paper, the response of 3T3 NIH fibroblasts to 2D planar surfaces versus their response to nanofiber mat surfaces, which have 3D effects, are investigated. A difference in cell shape from triangular cell body with prominent vertices to triangular cell body with blunted vertices and increased cell-cell adhesions at such vertices was observed using fluorescent microscopy, optical microscopy and atomic force microscopy. A new and powerful atomic force microscopy technique developed by our group, Scanning Probe Recognition Microscopy, is introduced and implemented to directly recognize and auto-focus on fibroblast vertex regions.

Keywords

biomedicalnanoscalescanning probe microscopy (SPM)
Type
Slide Presentations
Information
MRS Online Proceedings Library (OPL) , Volume 1019: Symposium FF – Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease , 2007 , 1019-FF06-04
Copyright
Copyright © Materials Research Society 2007

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References

1. Svitkina, T.M. and Borisy, G.G., Lamellipodia. J. Cell Biol., 145, 1009–26 (1999).10.1083/jcb.145.5.1009Google Scholar
2. Svitkina, T.M., Bulanova, E.A., Chaga, O.Y., J. Cell Biol., 160, 409–21 (2003)10.1083/jcb.200210174Google Scholar
3. Deligianni, D. D., Katsala, N. D., Koutsoukos, P. G., and Missirlis, Y. F., Biomaterials, 22, 87–96 (2001).Google Scholar
4. Dalby, Matthew John, Riehle, Mathis O., Sutherland, Duncan S, Agheli, Hossein, Curtis, Adam SG, Biomaterials, 25:2323, 5415–5422 (2004).Google Scholar
5. Discher, D. E., Janmey, P., and Wang, Y.-l., Science, 310, 1139–1143 (2005).10.1126/science.1116995Google Scholar
6. Sawada, Yasuhiro and Sheetz, Michael P., J. Cell Biology, 156, 609–615 (2002).10.1083/jcb.200110068Google Scholar
7. Fan, Y., Chen, Q., Ayres, V.M., Baczewski, A.D., Udpa, L. and Kumar, S., Int. J. Nanomedicine, in press.Google Scholar
8. http://www.egr.msu.edu/ebnl, then Research, Scanning Probe Recognition Microscopy for an SPRM video demonstration.Google Scholar
9. Nur-E-Kamal, A., Ahmed, I., Kamal, J., Schindler, M., Meiners, S., Stem Cells 24 426–433 (2005)10.1634/stemcells.2005-0170Google Scholar
10. Ray, RM, Vaidya, RJ, and Johnson, LR, Cell Motil Cytoskeleton 64 143–156 (2007)10.1002/cm.20172Google Scholar