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Breast cancer detection using charge sensors coupled to DNA monolayer

Published online by Cambridge University Press:  02 July 2015

Marina R. Batistuti ,
Marcelo Mulato  and
Paulo R. Bueno
Marina R. Batistuti
Affiliation:
Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901 Ribeirão Preto, Sao Paulo, Brazil
Marcelo Mulato
Affiliation:
Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901 Ribeirão Preto, Sao Paulo, Brazil
Paulo R. Bueno
Affiliation:
Instituto de Química, Universidade Estadual Paulista, CP 355, 14800-900, Araraquara, São Paulo, Brazil
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Abstract

We report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (Rct) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCl2 influence on ssDNA immobilization solution.

Keywords

sensornanostructureself-assembly
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1793: Symposium GG – Foundations of Bio/Nano Interfaces―Synthesis, Modeling, Design Principles and Applications , 2015 , pp. 19 - 26
Copyright
Copyright © Materials Research Society 2015 

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