Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T01:12:27.875Z Has data issue: false hasContentIssue false
  • English
  • Français

Biotinylated Polythiophene Copolymer – a Novel Electroactive Biomaterial Utilizing the Biotin-Streptavidin Interaction

Published online by Cambridge University Press:  15 February 2011

Jeong-Ok Lim ,
Manjunath Kamath ,
Kenneth A. Marx ,
Sukant K. Tripathy ,
David L. Kaplan  and
Lynne A. Samuelson
Jeong-Ok Lim
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Manjunath Kamath
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Kenneth A. Marx
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Sukant K. Tripathy
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
David L. Kaplan
Affiliation:
Biotechnology Division, U.S. Army Natick Research, Development & Engineering Center, Natick, Massachusetts 01760
Lynne A. Samuelson
Affiliation:
Biotechnology Division, U.S. Army Natick Research, Development & Engineering Center, Natick, Massachusetts 01760
Get access

Abstract

A novel hierarchical biomaterial capable of incorporating any biotinylated biomolecule has been created. Our strategy is to biotinylate one-dimensional electroactive polymers and use a bridging streptavidin protein on Langmuir-Blodgett (LB) organized films. The following copolymeric system which enables functionalization of other molecules and formation of good monolayers was employed. Biotinylated poly(3-methanolthiophene-co-3-undecylthiophene) (B-PMUT) demonstrated a significantly better isotherm implying superior molecular packing compared to poly(3-methanolthiophene-co-3-undecylthiophene) (PMUT) on the LB airwater surface. The isotherm showed significant area expansion when streptavidin was injected below the B-PMUT monolayer in 0.1mM NaH2PO4/0.1 M NaCl buffer (pH 6.8) subphase. We then incorporated biotinylated phycoerythrin (B-PE) into this novel biomaterial by binding the unoccupied biotin binding sites on the bound streptavidin (4 sites total). The pressure-area isotherm of the protein injected monolayer showed area expansion. A characteristic fluorescent emission peak at 576nm was detected from the monolayer transferred onto a solid substrate. These observations demonstrated the function of B-PMUT in hierarchical monolayer assembly of molecules incorporating the biotin / streptavidin interaction.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 292: Symposium S – Biomolecular Materials , 1992 , 141
Copyright
Copyright © Materials Research Society 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Nalwa, H.S., Die Angewandte Makromolekulare Chemie, 188,105 (1991).Google Scholar
2. Elsenbaumer, R.L., Jen, K.Y., Miller, G.G. and Shacklette, L.W., Synthetic Metals, 18, 277 (1987).Google Scholar
3. Naarmann, H., Adv. Matel., 2, 345 (1990).Google Scholar
4. Marder, S.R., Perry, J.W., Klavetter, F.L. and Grubbs, R.H., Chem.Mater., 1, 171 (1989).Google Scholar
5. Hotta, S., Synthetic Metals, 22, 103 (1987); 26, 267 (1988).Google Scholar
6. Hass, H. and Molhald, H., Thin Solid Films, 180, 101 (1990).Google Scholar
7. Gimlick, R.K. and Giese, R.W., J. Biol. Chem., 263, 210 (1980).Google Scholar
8. Samuelson, L. A., Miller, P., Galotti, D., Marx, K. A., Kumar, J., and Tripathy, S. K., Langmuir, 8, 604 (1992).Google Scholar
9. Blankenburg, R., Mellur, P., Ringsdorf, P. and Salesse, C., Biochem., 28, 8214 (1989).Google Scholar
10. Yamamoto, T., Sanechika, K., and Yamamoto, A., Bull. Chem. Soc. Jpn., 56, 1497 (1983).Google Scholar