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Biologically Engineered Quantum Dots for Biomedical Applications

Published online by Cambridge University Press:  01 February 2011

Ganna Chornokur ,
Sergei Ostapenko ,
Yusuf Emirov ,
Nadezhda Korsunska ,
Abraham Wolcott ,
Jin Zhang ,
Catherine Phelan ,
Abhilasha Nagaram  and
Thomas Sellers
Ganna Chornokur
Affiliation:
[email protected], University of South Florida, Nanomaterials and Nanomanufacturing Research Center, Building/Room NTA 102, Mailstop ENB 118, 4202 E. Fowler Ave, tampa, FL, 33620, United States
Sergei Ostapenko
Affiliation:
[email protected], University of South Florida, Nanomaterials and Nanomanufacturing Research Center, ENB 118, 4202 E. Fowler Ave, Tampa, FL, 33620, United States
Yusuf Emirov
Affiliation:
[email protected], University of South Florida, Nanomaterials and Nanomanufacturing Research Center, ENB 118, 4202 E. Fowler Ave, Tampa, FL, 33620, United States
Nadezhda Korsunska
Affiliation:
[email protected], Institute of Semiconductor Physics, Ukrainian Academy of Sciences, pr. Nauki 45, Kiev, N/A, Ukraine
Abraham Wolcott
Affiliation:
[email protected], University of California Santa Cruz, Santa Cruz, CA, 95064, United States
Jin Zhang
Affiliation:
[email protected], University of California Santa Cruz, Santa Cruz, CA, 95064, United States
Catherine Phelan
Affiliation:
[email protected], H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33620, United States
Abhilasha Nagaram
Affiliation:
[email protected], H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33620, United States
Thomas Sellers
Affiliation:
[email protected], H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33620, United States
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Abstract

We report on a short-wavelength, “blue” spectral shift of the photoluminescence (PL) spectrum in CdSeTe/ZnS core/shell quantum dots (QDs) caused by bioconjugation with several monoclonal cancer related antibodies (ABs). Scanning PL spectroscopy was performed on samples dried on solid substrates at various temperatures. The influence of the AB chemical origin on the PL spectral shift was observed. The conjugation QD-AB reaction was confirmed using the agarose gel electrophoresis technique. The spectral shift is strongly increased and the process facilitated when the samples are dried above room temperature. The PL spectroscopic mapping revealed a profile of the PL spectral shift across the dried QD-AB spot. Transmission Electron Microscopy analyses of the samples were performed to reveal the shape and size of individual QDs. A mechanism of the “blue” shift is attributed to changes in the QD electronic energy levels caused by local stress field applied to the bio-conjugated QD.

Keywords

biomedicallaserluminescence
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1095: Symposium EE – Responsive Biomaterials for Biomedical Applications , 2008 , 1095-EE08-05
Copyright
Copyright © Materials Research Society 2008

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