Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-20T00:06:56.739Z Has data issue: false hasContentIssue false
  • English
  • Français

Bio-hybrid photoconverter by covalent functionalization of the photosynthetic reaction center of Rhodobacter sphaeroides with fluorescein isothiocyanate

Published online by Cambridge University Press:  16 June 2015

Rocco Roberto Tangorra ,
Alessandra Antonucci ,
Francesco Milano ,
Simona la Gatta ,
Alessandra Operamolla ,
Roberta Ragni ,
Angela Agostiano ,
Massimo Trotta  and
Gianluca M. Farinola
Rocco Roberto Tangorra
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy.
Alessandra Antonucci
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy.
Francesco Milano
Affiliation:
Istituto per i Processi Chimico Fisici, Consiglio Nazionale delle Ricerche, Via Orabona, 4, 70126 Bari, Italy.
Simona la Gatta
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy.
Alessandra Operamolla
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy.
Roberta Ragni
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy.
Angela Agostiano
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy. Istituto per i Processi Chimico Fisici, Consiglio Nazionale delle Ricerche, Via Orabona, 4, 70126 Bari, Italy.
Massimo Trotta
Affiliation:
Istituto per i Processi Chimico Fisici, Consiglio Nazionale delle Ricerche, Via Orabona, 4, 70126 Bari, Italy.
Gianluca M. Farinola
Affiliation:
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro. Via Orabona, 4, 70126 Bari, Italy. Istituto di Chimica dei Composti Organometallici, Consiglio Nazionale delle Ricerche, Via Orabona, 4, 70126 Bari, Italy.
Get access

Abstract

Organic biological hybrid systems, accessible by covalent functionalization of photosynthetic proteins with molecular antennas, represent a promising novelty to enhance natural photosynthesis. In this paper, we present the successful bioconjugation of a commercial fluorophore, fluorescein isothiocyanate (FITC), to the photosynthetic reaction center RC from the bacterium Rhodobacter sphaeroides strain R26. The resulting hybrid outperforms the pristine protein in hole-electron couple generation yield, exclusively at wavelengths where the fluorophore absorbs while the protein does not.

Keywords

biological synthesis (assembly)biological synthesis (chemical reaction)biomimetic (assembly)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1722: Symposium F – Reverse Engineering of Bioinspired Nanomaterials , 2015 , mrsf14-1722-f05-51
Copyright
Copyright © Materials Research Society 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Maróti, P. and Trotta, M., in CRC Handbook of Organic Photochemistry and Photobiology, Third Edition - Two Volume Set (CRC Press, 2012), pp. 1289–1324.CrossRefGoogle Scholar
Milano, F., Tangorra, R. R., Hassan Omar, O., Ragni, R., Operamolla, A., Agostiano, A., Farinola, G. M. and Trotta, M., Angewandte Chemie 124 (44), 11181–11185 (2012).CrossRefGoogle Scholar
Ragni, R., Omar, O. H., Tangorra, R. R., Milano, F., Vona, D., Operamolla, A., La Gatta, S., Agostiano, A., Trotta, M. and Farinola, G. M., MRS Online Proceedings Library 1689 (2014).Google Scholar
Stowell, M. H. B., McPhillips, T. M., Rees, D. C., Soltis, S. M., Abresch, E. and Feher, G., Science 276 (5313), 812-816 (1997).CrossRefGoogle Scholar
Dutta, P. K., Levenberg, S., Loskutov, A., Jun, D., Saer, R., Beatty, J. T., Lin, S., Liu, Y., Woodbury, N. W. and Yan, H., J Am Chem Soc 136 (47), 16618–16625 (2014).CrossRefGoogle Scholar
Dutta, P. K., Lin, S., Loskutov, A., Levenberg, S., Jun, D., Saer, R., Beatty, J. T., Liu, Y., Yan, H. and Woodbury, N. W., J Am Chem Soc 136 (12), 4599–4604 (2014).CrossRefGoogle Scholar
Isaacson, R. A., Lendzian, F., Abresch, E. C., Lubitz, W. and Feher, G., Biophys J 69 (2), 311–322. (1995).CrossRefGoogle Scholar
Farmer, W. H. and Yuan, Z., Anal Biochem 197 (2), 347–352 (1991).CrossRefGoogle Scholar
Milano, F., Agostiano, A., Mavelli, F. and Trotta, M., Eur J Biochem 270 (23), 4595–4605 (2003).CrossRefGoogle Scholar