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Bioactive Glass Templates for the Synthesis of Bone-Like Tissue In Vitro

Published online by Cambridge University Press:  15 February 2011

A. Ei-Ghannam ,
P. Ducheyne  and
I. M. Shapiro
A. Ei-Ghannam
Affiliation:
Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania Philadelphia, PA 19104 U.S.A.
P. Ducheyne
Affiliation:
Department of Bioengineering, School of Engineering and Applied Science University of Pennsylvania Philadelphia, PA 19104 U.S.A.
I. M. Shapiro
Affiliation:
Department of Biochemistry, School of Dental Medicine University of Pennsylvania Philadelphia, PA 19104 U.S.A.
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Abstract

Porous glass templates of pore size 75–200 14m and 20–30% porosity were synthesized. The glass disks were conditioned in a modified tris buffer (SBF) for 48 hrs and then treated with tissue culture medium (TCM) for 1 hr at 37°C. Other porous glass templates were treated with either SBF or TCM. The conditioned glass disks were seeded with 106 neonatal rat calvaria osteoblasts and maintained in culture for 2, 5 or 7 days. It was found that the bioactive glass was rapidly invaded by cells which colonized the porous template. Morphological and biochemical analyses suggested that a bone-like tissue was formed inside templates conditioned with both SBF and TCM. By 7 days, the cells exhibited high alkaline phosphatase activity and synthesized osteocalcin. SDS-PAGE indicated the presence of type I collagen. SEM-EDAX analysis showed the formation of a bone-like tissue throughout the entire sample, while inspection of the Ca:P ratio indicated that the cells synthesized a mineral phase. Indeed, the FTIR spectra of the mineral confirmed that it was a biological hydroxyapatite. The rapid formation of the bone-like material within the template in vitro suggests that this template may be valuable clinically for the repair of bone lesions.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 257
Copyright
Copyright © Materials Research Society 1994

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