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An efficient urea biosensor based on laser ablated ZnO thin film

Published online by Cambridge University Press:  25 January 2013

Neha Batra ,
Monika Tomar  and
Vinay Gupta
Neha Batra
Affiliation:
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, INDIA
Monika Tomar
Affiliation:
Department of Physics, Miranda House, University of Delhi, Delhi 110007, INDIA
Vinay Gupta*
Affiliation:
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, INDIA
*
*Email id:[email protected]; [email protected]Contact no: +91 9811563101
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Abstract

Zinc oxide (ZnO) thin film deposited onto indium tin oxide (ITO) coated Corning glass substrates using pulsed laser deposition (PLD) technique has been used as a matrix for realization of an efficient urea biosensor after immobilization of urease (Urs) enzyme onto the surface of ZnO. The bioelectrode (Urs/ZnO/ITO/glass) is found to be exhibiting an enhanced sensitivity of 22μΑmΜ−1cm−2 towards urea over a wide detection range of 5-200 mg/dl. The relatively low value of Michaelis menten constant (Km= 0.94mM) indicates high affinity of the immobilized urease towards the analyte (urea). The prepared biosensor retains 90% of its activity for more than 10 weeks. The observed enhanced response characteristics of bioelectrode are attributed to the growth of the matrix (highly c-axis oriented ZnO thin film) with desired surface morphology and high electron communication feature. The results confirm the promising application of PLD grown ZnO thin film as an efficient matrix for urea detection.

Keywords

II-VIablationbiomaterial
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1530: Symposium XX – Materials and Concepts for Biomedical Sensing , 2013 , mrsf12-1530-xx07-24
Copyright
Copyright © Materials Research Society 2013

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