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Microengineering the Environment of Mammalian Cells in Culture

Published online by Cambridge University Press:  31 January 2011

Christopher S. Chen ,
Xingyu Jiang  and
George M. Whitesides
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Abstract

Assays based on observations of the biological responses of individual cells to their environment have the potential to make enormous contributions to cell biology and biomedicine.To carry out well-defined experiments using cells, both the environments in which the cells live and the cells themselves must be well defined. Cell-based assays are now plagued by inconsistencies and irreproducibility, and a primary challenge in the development of informative assays is to understand the fundamental bases for these inconsistencies and to limit them. It now seems that multiple factors may contribute to the variability in the response of individual cells to stimuli; some of these factors may be extrinsic to the cells, some intrinsic. New techniques based on microengineering—especially using soft lithography to pattern surfaces at the molecular level and to fabricate microfluidic systems—have provided new capabilities to address the extrinsic factors. This review discusses recent advances in materials science that provide well-defined physical environments that can be used to study cells, both individually and in groups, in attached culture. It also reviews the challenges that must be addressed in order to make cell-based assays reproducible.

Keywords

biosensorscell-based assaysdiagnosticsmicrofabricationmicrofluidicsPDMSpoly(dimethylsiloxane)soft lithography.
Type
Research Article
Information
MRS Bulletin , Volume 30 , Issue 3: Biointerface Science , March 2005 , pp. 194 - 201
Copyright
Copyright © Materials Research Society 2005

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References

1.Arthur, C., Guyton, M.D., and Hall, J.E., Textbook of Medical Physiology, 10th Ed. (W.B. Saunders, Philadelphia, 2000).Google Scholar
2.Chen, C.S., Tan, J., and Tien, J., Ann. Rev. Biomed. Eng. 6 (2004) p. 275.CrossRefGoogle Scholar
3.Alberts, B., Johnson, A., Lewis, J., Raff, M., Keith, R., and Walter, P., Molecular Biology of the Cell, 4th Ed. (Garland Science, New York, 2002).Google Scholar
4.Takayama, S., Ostuni, E., LeDuc, P., Naruse, K., Ingber, D.E., and Whitesides, G.M., Nature 411 (2001) p. 1016.CrossRefGoogle Scholar
5.Dertinger, S.K.W., Jiang, X., Li, Z., Murthy, V.N., and Whitesides, G.M., Proc. Natl. Acad. Sci. USA 99 (2002) p. 12542.CrossRefGoogle Scholar
6.Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X., and Ingber, D.E., Annu. Rev. Biomed. Eng. 3 (2001) p. 335.CrossRefGoogle Scholar
7.Gates, B.D., Xu, Q.B., Love, J.C., Wolfe, D.B., and Whitesides, G.M., Annu. Rev. Mater. Res. 34 (2004) p. 339.CrossRefGoogle Scholar
8.Jiang, X. and Whitesides, G.M., Eng. Life Sci. 3 (2003) p. 475.CrossRefGoogle Scholar
9.Kenis, P., Ismagilov, R., Takayama, S., and Whitesides, G., Acc. Chem. Res. 33 (2000) p. 841.CrossRefGoogle Scholar
10.Xia, Y. and Whitesides, G.M., Angew. Chem. Int. Ed. Engl. 37 (1998) p. 550.3.0.CO;2-G>CrossRefGoogle Scholar
11.Chen, C.S., Mrksich, M., Huang, S., Whitesides, G.M., and Ingber, D.E., Science 276 (1997) p. 1425.CrossRefGoogle Scholar
12.Takayama, S., McDonald, J.C., Ostuni, E., Liang, M.N., Kenis, P.J.A., Ismagilov, R.F., and Whitesides, G.M., Proc. Natl. Acad. Sci. USA 96 (1999) p. 5545.CrossRefGoogle Scholar
13.Chiu, D.T., Jeon, N.L., Huang, S., Kane, R.S., Wargo, C.J., Choi, I.S., Ingber, D.E., and Whitesides, G.M., Proc. Natl. Acad. Sci. USA 97 (2000) p. 2408.CrossRefGoogle Scholar
14.Dertinger, S.K.W., Chiu, D.T., Jeon, N.L., and Whitesides, G.M., Anal. Chem. 73 (2001) p. 1240.CrossRefGoogle Scholar
15.Huang, S., Chen, C.S., and Ingber, D.E., Mol. Biol. Cell. 9 (1998) p. 3179.CrossRefGoogle Scholar
16.McBeath, R., Pirone, D.M., Nelson, C.M., Bhadriraju, K., and Chen, C.S., Dev. Cell 6 (2004) p. 483.CrossRefGoogle ScholarPubMed
17.Tessier-Lavigne, M. and Goodman, C.S., Science 274 (1996) p. 1123.CrossRefGoogle Scholar
18.Brandley, B.K. and Schnaar, R.L., Dev. Biol. 135 (1989) p. 74.CrossRefGoogle Scholar
19.Bray, D., Cell Movements: from Molecules to Motility, 2nd Ed. (Garland Publishing, New York, 2001).Google Scholar
20.Van Haastert, P.J.M. and Devreotes, P.N., Nat. Rev. Mol. Cell Biol. 5 (2004) p. 626.CrossRefGoogle Scholar
21.Hong, K., Nishiyama, M., Henley, J., Tessier-Lavigne, M., and Poo, M.M., Nature 403 (2000) p. 93.CrossRefGoogle Scholar
22.Zackson, S.L. and Steinberg, M.S., Dev. Biol. 124 (1987) p. 418.CrossRefGoogle Scholar
23.McCarthy, J.B. and Furcht, L.T., J. Cell Biol. 98 (1984) p. 1474.CrossRefGoogle Scholar
24.Rigot, V., Lehmann, M., Andre, F., Daemi, N., Marvaldi, J., and Luis, J., J. Cell Sci. 111 (1998) p. 3119.CrossRefGoogle Scholar
25.Jeon, N.L., Baskaran, H., Dertinger, S., Whitesides, G.M., Van De Water, L., and Toner, M., Nat. Biotechnol. 20 (2002) p. 826.CrossRefGoogle Scholar
26.Ostuni, E., Kane, R., Chen, C.S., Ingber, D.E., and Whitesides, G.M., Langmuir 16 (2000) p. 7811.CrossRefGoogle Scholar
27.Folch, A., Jo, B.H., Hurtado, O., Beebe, D.J., and Toner, M., J. Biomed. Mater. Res. 52 (2000) p. 346.3.0.CO;2-H>CrossRefGoogle Scholar
28.Ingber, D.E., Proc. Natl. Acad. Sci. USA 87 (1990) p. 3579.CrossRefGoogle Scholar
29.Parker, K.K., Brock, A.L., Brangwynne, C., Mannix, R.J., Wang, N., Ostuni, E., Geisse, N.A., Adams, J.C., Whitesides, G.M., and Ingber, D.E., FASEB J. 16 (2002) p. 1195.CrossRefGoogle Scholar
30.Brock, A., Chang, E., Ho, C.-C., LeDuc, P., Jiang, X., Whitesides, G.M., and Ingber, D.E., Langmuir 19 (2003) p. 1611.CrossRefGoogle Scholar
31.Tan, J.L., Tien, J., Pirone, D.M., Gray, D.S., Bhadriraju, K., and Chen, C.S., Proc. Natl. Acad. Sci. USA 100 (2003) p. 1484.CrossRefGoogle Scholar
32.Lo, C.M., Wang, H.B., Dembo, M., and Wang, Y.L., Biophys. J. 79 (2000) p. 144.CrossRefGoogle Scholar
33.Yousaf, M.N., Houseman, B.T., and Mrksich, M., Angew. Chem. Int. Ed. 40 (2001) p. 1093.3.0.CO;2-Q>CrossRefGoogle Scholar
34.Yeo, W.-S., Yousaf, M.N., and Mrksich, M., J. Am. Chem. Soc. 125 (2003) p. 14994.CrossRefGoogle Scholar
35.Jiang, X., Ferrigno, R., Mrksich, M., and Whitesides, G.M., J. Am. Chem. Soc. 125 (2003) p. 2366.CrossRefGoogle Scholar
36.Nelson, C.M. and Chen, C.S., FEBS Lett. 514 (2002) p. 238.CrossRefGoogle Scholar
37.Singhvi, R., Kumar, A., Lopez, G.P., Stephanopoulos, G.N., Wang, D.I.C., Whitesides, G.M., and Ingber, D.E., Science 264 (1994) p. 696.CrossRefGoogle Scholar