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Microcrystal electron diffraction methodology and applications

Published online by Cambridge University Press:  10 December 2019

Brent L. Nannenga  and
Tamir Gonen
Brent L. Nannenga
Affiliation:
School for Engineering Matter, Transport and Energy, Arizona State University, USA; [email protected]
Tamir Gonen
Affiliation:
Howard Hughes Medical Institute, Departments of Biological Chemistry and Physiology, University of California, Los Angeles, USA; [email protected]
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Abstract

Microcrystal electron diffraction (MicroED) is a cryo-electron microscopy technique that utilizes three-dimensional nano- and microcrystals for high-resolution structure determination. These extremely small crystals are several orders of magnitude smaller than what is used in conventional x-ray diffraction experiments. MicroED is capable of providing high-quality data from samples that would otherwise be considered useless for diffraction measurements. Since its initial implementation, MicroED has been used in the fields of structural biology, chemistry, and materials science. In this article, we provide an overview of the MicroED methodology as well as examples of how MicroED in cryo-electron microscopy has been used for structure determination of a variety of samples.

Type
Cryogenic Electron Microscopy in Materials Science
Information
MRS Bulletin , Volume 44 , Issue 12: Cryogenic Electron Microscopy in Materials Science , December 2019 , pp. 956 - 960
Copyright
Copyright © Materials Research Society 2019 

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