Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-26T09:14:12.484Z Has data issue: false hasContentIssue false
  • English
  • Français

Engineering Enzymes and Antibodies

Published online by Cambridge University Press:  29 November 2013

Donald Hilvert
Get access

Extract

Phenomenal rate accelerations, exacting selectivities, and mild reaction conditions characterize biocatalysis and have generated considerable interest in nonbiological applications of enzymes. As a result of greater availability and purity, these molecules are now being used increasingly in organic synthesis to prepare complex natural products and novel materials. They are also being exploited industrially in the production of food additives, pharmaceuticals, and fine chemicals.

Unfortunately, for many commercial applications, natural enzymes may be unsuitable. They may be unstable or difficult to isolate; they may function poorly at the temperatures, pH's, and substrate concentrations needed for reaction; or they may lack appropriate specificity. For many chemical reactions, a natural enzyme may not even exist. For example, natural biocatalysts for the synthetically valuable Diels-Alder cycloaddition have yet to be discovered.

Type
Biology and Materials Synthesis
Information
MRS Bulletin , Volume 17 , Issue 11 , November 1992 , pp. 48 - 52
Copyright
Copyright © Materials Research Society 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Wong, C-H., Science 244 (1989) p. 1145.CrossRefGoogle Scholar
2.Gerhartz, W., Enzymes in Industry: Production and Applications (VCH, New York, 1990).Google Scholar
3.Fersht, A., Enzyme Structure and Mechanism (W.H. Freeman and Company, New York, 1985).Google Scholar
4.Knowles, J.R., Science 236 (1987) p. 1252; J.A. Gerlt, Chem. Rev. 87 (1987) p. 1079.CrossRefGoogle Scholar
5.Kaiser, E.T., Lawrence, D.S., and Rokita, S.E., Annu. Rev. Biochem. 54 (1985) p. 565.CrossRefGoogle Scholar
6.Wells, J.A. and Estell, D.A., Trends Biochem. Sci. 13 (1988) p. 291.CrossRefGoogle Scholar
7.Carter, P. and Wells, J.A., Nature 332 (1988) p. 564.CrossRefGoogle Scholar
8.Philipp, M. and Bender, M.L., Mol. Cell. Biochem. 51 (1983) p. 5; and references therein.CrossRefGoogle Scholar
9.Nakatsuka, T., Sasaki, T., and Kaiser, E.T., J. Am. Chem. Soc. 109 (1987) p. 3808.CrossRefGoogle Scholar
10.Abrahmsén, L., Tom, J., Burnier, J., Butcher, K.A., Kossiakoff, A., and Wells, J.A., Biochem. 30 (1991) p. 4151.CrossRefGoogle Scholar
11.Wu, Z-P. and Hilvert, D., J. Am. Chem. Soc. 111 (1989) p. 4513.CrossRefGoogle Scholar
12.Wu, Z-P. and Hilvert, D., J. Am. Chem. Soc. 112 (1990) p. 5647.CrossRefGoogle Scholar
13.Noren, C.J., Anthony-Cahill, S.J., Griffith, M.C., and Schultz, P.G., Science 244 (1989) p. 182; J.D. Bain, C.G. Glabe, T.A. Dix, A.R. Chamberlin, and E.S. Diala, J. Am. Chem. Soc. 111 (1989) p. 8013.CrossRefGoogle Scholar
14.Stadtman, T.C., Annu. Rev. Biochem. 59 (1990) p. 111.CrossRefGoogle Scholar
15.Wells, J.A., Cunningham, B.C., Graycar, T.P. and Estell, D.A., Proc. Natl Acad. Sci. U.S.A. 84 (1987) p. 5167.CrossRefGoogle Scholar
16.Estell, D.A., Graycar, T.P., Miller, J.V., Powers, D.B., Burnier, J.P., Ng, P.G., and Wells, J.A., Science 233 (1986) p. 659.CrossRefGoogle Scholar
17.Wells, J.A., Powers, D.B., Bott, R.R., Graycar, T.P., and Estell, D.A., Proc. Natl. Acad. Sci. U.S.A. 84 (1987) p. 1219.CrossRefGoogle Scholar
18.Carter, P. and Wells, J.A., Science 237 (1987) p. 394.CrossRefGoogle Scholar
19.Kennedy, J.F. and Cabral, J.M.S., in Biotechnol, Vol. 7a, edited by Kennedy, J.F. (VCH, New York, 1987) p. 347.Google Scholar
20.Hilvert, D., Trends Biotechnol. 9 (1991) p. 11; and references therein.CrossRefGoogle Scholar
21.Zhong, Z., Liu, J.L-C., Dinterman, L.M., Finkelman, M.A.J., Mueller, W.T., Rollence, M.L., Whitlow, M., and Wong, C-H., J. Am. Chem. Soc. 113 (1991) p. 683.CrossRefGoogle Scholar
22.Bott, R.R., Ultsch, M., Kossiakoff, A., Graycar, T., Katz, B., and Power, S., J. Biol. Chem. 263 (1988) p. 7895.CrossRefGoogle Scholar
23.Estell, D.A., Graycar, T.P., and Wells, J.A., J. Biol. Chem. 260 (1985) p. 6518.CrossRefGoogle Scholar
24.Alt, F.W., Blackwell, T.K., and Yancopoulos, G.D., Science 238 (1987) p. 1079; K. Rajewsky, I. Förster, and A. Cumano, Science 238 (1987) p. 1088.CrossRefGoogle Scholar
25.Lerner, R.A., Benkovic, S.J., and Schultz, P.G., Science 252 (1991) p. 659; C. Lewis and D. Hilvert, Current Opin. Struct. Biol. 1 (1992) p. 624.CrossRefGoogle Scholar
26.Kitazume, T., Lin, J.T., Yamamoto, T., and Yamazaki, T., J. Am. Chem. Soc. 113 (1991) p. 8573.CrossRefGoogle Scholar
27.Fernholz, E., Schloeder, D., Liu, K.K.C., Bradshaw, C.W., Huang, H., Janda, K., Lerner, R.A., and Wong, C-H., J. Org. Chem. 57 (1992) p. 4756.CrossRefGoogle Scholar
28.Blackburn, G.F., Talley, D.B., Booth, P.M., Durfor, C.N., Martin, M.T., Napper, A.D., and Rees, A.R., Anal. Chem. 62 (1990) p. 2211.CrossRefGoogle Scholar
29.Benkovic, S.J., Adams, J.A., Borders, C.L., Janda, K.D., and Lerner, R.A., Science 250 (1990) p. 1135.CrossRefGoogle Scholar
30.Janda, K.D., Schloeder, D., Benkovic, S.J., and Lerner, R.A., Science 241 (1988) p. 1188.CrossRefGoogle Scholar
31.Janda, J.D., Weinhouse, M.I., Schloeder, D.M., Lerner, R.A., and Benkovic, S.J., J. Am. Chem. Soc. 112 (1990) p. 1274.CrossRefGoogle Scholar
32.Jackson, D.Y., Prudent, J.R., Baldwin, E.P., and Schultz, P.G., Proc. Natl. Acad. Sci. U.S.A. 88 (1991) p. 58.CrossRefGoogle Scholar
33.Pollack, S.J. and Schultz, P.G., J. Am. Chem. Soc. 111 (1989) p. 1929.CrossRefGoogle Scholar
34.Iverson, B.L. and Lerner, R.A., Science 243 (1989) p. 1184.CrossRefGoogle Scholar
35.Hilvert, D., Hill, K.W., Nared, K.D., and Auditor, M.T.M., J. Am. Chem. Soc. 111 (1989) p. 9261; A.C. Braisted and P.G. Schultz, J. Am. Chem. Soc. 112 (1990) p. 7430.CrossRefGoogle Scholar
36.Lewis, C., Kramer, T., Robinson, S., and Hilvert, D., Science 253 (1991) p. 1019.CrossRefGoogle Scholar
37.Tang, Y., Hicks, J.B., and Hilvert, D., Proc. Natl. Acad. Sci. U.S.A. 88 (1991) p. 8784.CrossRefGoogle Scholar
38.Chiswell, D.J. and McCafferty, J., Trends Biotechnol. 10 (1992) p. 80.CrossRefGoogle Scholar