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Three-Dimensional Scaffold Fabrication with Inverse Photolithography

Published online by Cambridge University Press:  15 December 2016

Ramesh Prashad  and
Ozlem Yasar
Ramesh Prashad
Affiliation:
Department of Mechanical Engineering Technology, New York City College of Technology, Brooklyn, NY 11201, USA.
Ozlem Yasar*
Affiliation:
Department of Mechanical Engineering Technology, New York City College of Technology, Brooklyn, NY 11201, USA.
*
*(Email: [email protected])
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Abstract

In recent years, tissue engineering has been utilized as an alternative approach to organ transplantation. Success rate of tissue regeneration influenced by the biomaterials, cell sources, growth factors and scaffold fabrication. Design and precise fabrication of scaffolds are required to support cells to expand and migrate to 3D environment. Common scaffold fabrication techniques use heat, adhesives, molds or light. In this research, “inverse-photolithography” which is a light based fabrication technique was used to generate the scaffolds. In order to control the interior architecture of the scaffold “a single vertical strut” and “a y-shape” were fabricated with the 3D printer by using the dissolvable filament. Then, the strut and the y-shape were immersed into the photo-curable solution which is poly(ethylene glycol) diacrylate (PEGDA) and photo-initiator mixture. UV light with the 365nm wavelength was placed up-side down under the solution. Photo-curable mixture was exposed to the UV light for 3 minutes to cure the entire scaffold. Solidified scaffold with the strut and y-shape inside was kept in the limonene solution. Limonene penetrated through the open ended strut and y-shape and it dissolved the 3D printed strut and y-shape away leaving the fabricated PEGDA based scaffolds. This preliminary research showcases, the 3D scaffolds with the controlled interior design, can be fabricated with the “inverse-photolithography” technique.

Keywords

biomaterialpolymerizationtissue
Type
Articles
Information
MRS Advances , Volume 2 , Issue 19-20: Biomaterials and Soft Materials , 2017 , pp. 1071 - 1075
Copyright
Copyright © Materials Research Society 2016 

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References

REFERENCES

Hutmacher, D.W., Scaffold design and fabrication technologies for engineering tissues—state of the art and future perspectives . Journal of Biomaterials Science, Polymer Edition, 2001. 12(1): p. 107–124.Google Scholar
Arcaute, K., Mann, B.K., and Wicker, R.B., Stereolithography of three-dimensional bioactive poly (ethylene glycol) constructs with encapsulated cells . Annals of biomedical engineering, 2006. 34(9): p. 1429–1441.CrossRefGoogle ScholarPubMed
Yasar, O., Lan, S.F., and Starly, B.. Dependance of Lindenmayer System (L-System) Parameters on Flow Characteristics in Engineered Biomaterials. in ASME 2009 International Mechanical Engineering Congress and Exposition . 2009. American Society of Mechanical Engineers.Google Scholar
Nemir, S., Hayenga, H.N., and West, J.L., PEGDA hydrogels with patterned elasticity: novel tools for the study of cell response to substrate rigidity . Biotechnology and bioengineering, 2010. 105(3): p. 636–644.Google Scholar
Priola, A., et al., Properties of polymeric films obtained from uv cured poly (ethylene glycol) diacrylates . Polymer, 1993. 34(17): p. 3653–3657.Google Scholar
Khandaker, M., et al., Biomechanical Performances of Networked Polyethylene Glycol Diacrylate: Effect of Photoinitiator Concentration, Temperature, and Incubation Time . International journal of biomaterials, 2016. 2016.Google ScholarPubMed
O’brien, F.J., Biomaterials & scaffolds for tissue engineering . Materials today, 2011. 14(3): p. 88–95.Google Scholar
Yasar, O., Lan, S.-F., and Starly, B., A Lindenmayer system-based approach for the design of nutrient delivery networks in tissue constructs . Biofabrication, 2009. 1(4): p. 045004.Google Scholar
Illescas, J., et al., PEGDA-based luminescent polymers prepared by frontal polymerization . Journal of Polymer Science Part A: Polymer Chemistry, 2015. 53(24): p. 2890–2897.Google Scholar
Yasar, O., et al., Layered Fabrication of Branched Networks Using Lindenmayer Systems. University of Oklahoma, 2007.Google Scholar
Sun, C., et al., Projection micro-stereolithography using digital micro-mirror dynamic mask . Sensors and Actuators A: Physical, 2005. 121(1): p. 113–120.Google Scholar
Yasar, O. and Starly, B., Fabrication of Lindenmayer System-Based Designed Engineered Scaffolds Using UV-Maskless Photolithography . MRS Advances, 2016. 1(11): p. 749–754.Google Scholar
Yasar, O., A lindenmayer system-based approach for the design and fabrication of nutrient delivery networks in tissue constructs. 2011: THE UNIVERSITY OF OKLAHOMA.Google Scholar
Cuchiara, M.P., et al., Multilayer microfluidic PEGDA hydrogels . Biomaterials, 2010. 31(21): p. 5491–5497.Google Scholar
Li, Y., Rodrigues, J., and Tomas, H., Injectable and biodegradable hydrogels: gelation, biodegradation and biomedical applications . Chemical Society Reviews, 2012. 41(6): p. 2193–2221.Google Scholar
Yasar, O., et al. . Fabrication of Micropatterned Hydrogels Using Maskless Photopolymerization for Tissue Engineering Applications. in ASME 2008 Summer Bioengineering Conference . 2008. American Society of Mechanical Engineers.Google Scholar