Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-22T20:32:42.821Z Has data issue: false hasContentIssue false
  • English
  • Français

Tailoring super-hydrophobic properties of electrochemical biosensor for early cancer detection

Published online by Cambridge University Press:  05 August 2016

Natalia Malara ,
Francesco Gentile ,
Lorenzo Ferrara ,
Marco Villani ,
Salvatore Iannotta ,
Andrea Zappettini ,
Enzo Di Fabrizio ,
Valentina Trunzo ,
Vincenzo Mollace  and
Nicola Coppedé
Natalia Malara*
Affiliation:
Department of Health Science, University of Magna Graecia, 88100Catanzaro, Italy Department of Experimental and Clinical Medicine, University of Magna Graecia, 88100Catanzaro, Italy
Francesco Gentile
Affiliation:
Department of Electrical Engineering and Information Technology, University Federico II, Naples, Italy
Lorenzo Ferrara
Affiliation:
Istituto Italiano di Tecnologia, Via Morego 30, 16163Genova, Italy
Marco Villani
Affiliation:
IMEM-CNR Parco Area delle Scienze 37/A - 43124 Parma, Italy
Salvatore Iannotta
Affiliation:
IMEM-CNR Parco Area delle Scienze 37/A - 43124 Parma, Italy
Andrea Zappettini
Affiliation:
IMEM-CNR Parco Area delle Scienze 37/A - 43124 Parma, Italy
Enzo Di Fabrizio
Affiliation:
Department of Experimental and Clinical Medicine, University of Magna Graecia, 88100Catanzaro, Italy King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Valentina Trunzo
Affiliation:
Department of Health Science, University of Magna Graecia, 88100Catanzaro, Italy
Vincenzo Mollace
Affiliation:
Department of Health Science, University of Magna Graecia, 88100Catanzaro, Italy
Nicola Coppedé
Affiliation:
IMEM-CNR Parco Area delle Scienze 37/A - 43124 Parma, Italy
*
*corresponding author: [email protected]
Get access

Abstract

In this paper, we demonstrate an organic electrochemical transistor (OECT) based on the conductive polymer PEDOT:PSS for the analysis of the cell culture medium upon interaction with circulating cells isolated form peripheral blood sampling of health, sub-clinical and cancer patients. The device comprises arrays of super-hydrophobic micro-pillars in which a finite number of pillars incorporates nano-electrodes for site specific measurements of a solution. Due to its nano-scale architecture, the device realizes time and space resolved measurement of biological solution. Tumor metabolism could produce reactive species able to determine a different electronic behavior of correspondent microenviroment. On this basis, the device here presented the changes in the ESR signals was used to identify electronic changes occurring in the analysis of different type of microenvironment. Our results demonstrate that the device is able to register significative difference to differentiate healthy individuals form cancer patients, through an easy blood sampling. In conclusion, these preliminary data are suggestive of a novel test potentially useful to early identification of subjects at risk to development cancer disease.

Keywords

biomedicaldevicesbiomaterial
Type
Articles
Information
MRS Advances , Volume 1 , Issue 52: Soft Materials and Biomaterials , 2016 , pp. 3545 - 3552
Copyright
Copyright © Materials Research Society 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Dimitrakopoulos, C. D. and Malenfant, P. R. L., "Organic Thin Film Transistors for Large Area Electronics," Advanced Materials, vol. 14, pp. 99–117, 2002.3.0.CO;2-9>CrossRefGoogle Scholar
Owens, R. M. and Malliaras, G. G., "Organic Electronics at the Interface with Biology," MRS Bulletin, vol. 35, pp. 449–456, 2010.Google Scholar
Tarabella, G., Santato, C., Yang, S. Y., Iannotta, S., Malliaras, G. G., and Cicoira, F., "Effect of the gate electrode on the response of organic electrochemical transistors," Applied Physics Letters, vol. 97, p. 123304, 2010.CrossRefGoogle Scholar
Coppedè, N., Villani, M., and Gentile, F., "Diffusion Driven Selectivity in Organic Electrochemical Transistors," Scientific Reports, vol. 4, pp. 1–7, 2014.Google Scholar
Gentile, F., Coppedè, N., Tarabella, G., Villani, M., Calestani, D., Candeloro, P., et al., "Microtexturing of the Conductive PEDOT: PSS Polymer for Superhydrophobic Organic Electrochemical Transistors," BioMed Research International, pp. 1–10, 2014.Google Scholar
Gentile, F., Coluccio, M. L., Rondanina, E., Santoriello, S., Di Mascolo, D., Accardo, A., et al., "Non periodic patterning of super-hydrophobic surfaces for the manipulation of few molecules," Microelectronic Engineering, vol. 111, pp. 272–276, 2013.Google Scholar
Gentile, F., Das, G., Coluccio, M., Mecarini, F., Accardo, A., Tirinato, L., et al., "Ultra low concentrated molecular detection using super hydrophobic surface based biophotonic devices," Microelectronic Engineering, vol. 87 pp. 798–801, 2010.CrossRefGoogle Scholar
Tam, D., von Arnim, V., McKinley, G. H., and Hosoi, A. E., "Marangoni convection in droplets on superhydrophobic surfaces," Journal of Fluid Mechanics, vol. 624, pp. 101–123, 2009.CrossRefGoogle Scholar
Astier, Y., Data, L., Carney, R. P., Stellacci, F., Gentile, F., and Di Fabrizio, E., "Artificial Surface-Modified Si3N4 Nanopores for Single Surface-Modified Gold Nanoparticle Scanning," Small, vol. 7, pp. 455–459, 2010.CrossRefGoogle Scholar
Gentile, F., Coluccio, M. L., Zaccaria, R. P., Francardi, M., Cojoc, G., Perozziello, G., et al., "Selective on site separation and detection of molecules in diluted solutions with superhydrophobic clusters of plasmonic nanoparticles," Nanoscale, vol. 6, pp. 8208–8225, 2014.Google Scholar
Kalapos, M. P., “On the promine/retine theory of cell division: now and then,” Biochimica et Biophysica Acta (BBA) - General Subjects; vol 1426, Issue 1, pp.1–16, 1999.Google Scholar
Piskorska, D., Grabowska-Bochenek, R., “Role of glyoxalases and methylglyoxal in cell proliferation and differentiation,” Postepy Hig Med Dosw, vol. 49, issue 3, pp. 433–44, 1995.Google Scholar
Malara, N., Coluccio, M., Limongi, T., Asande, M., Trunzo, V., Cojoc, G., et al., "Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells," Small, vol. 10, pp. 4324–31, 2014.CrossRefGoogle Scholar
Malara, N., Trunzo, V., Foresta, U., Amodio N, N., De Vitis S, S., Roveda, L, et al., “Ex-vivo characterization of circulating colon cancer cells distinguished in stem and differentiated subset provides useful biomarker for personalized metastatic risk assessment,” J Transl Med. , vol. 14:133, pp. 1–12, 2016.Google Scholar
Malara, N., Givigliano, F., Trunzo, V., Macrina, L., Raso, C., Amodio, N., et al. , “In vitro expansion of tumour cells derived from blood and tumour tissue is useful to redefine personalized treatment in non-small cell lung cancer patients,” J Biol Regul Homeost Agents, vol. 28(4), pp. 717–31, 2014.Google Scholar
Gentile, F., Ferrara, L., Villani, M., Bettelli, M., Iannotta, S., Zappettini, A., et al. , "Geometrical Patterning of Super-Hydrophobic Biosensing Transistors Enables Space and Time Resolved Analysis of Biological Mixtures," Scientific Reports, vol. 6, pp. 1–15, 2016.Google Scholar