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Preparation and Characterization of Chitosan Microspheres for Controlled Release of Rh-BMP-2

Published online by Cambridge University Press:  18 November 2019

Cindy Sinaí Velázquez-González ,
Ena Athenea Aguilar-Reyes ,
Rosa Elvira Núñez-Anita  and
Carlos Alberto León-Patiño Open the ORCID record for Carlos Alberto León-Patiño [Opens in a new window]
Cindy Sinaí Velázquez-González
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
Ena Athenea Aguilar-Reyes*
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
Rosa Elvira Núñez-Anita
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Unidad de Proteómica y Bioingeniería Celular, Universidad Michoacana de San Nicolás de Hidalgo, Tarímbaro, México.
Carlos Alberto León-Patiño
Affiliation:
Instituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México.
*
*Corresponding author: Dr. Ena Athenea Aguilar Reyes (Email: [email protected])
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Abstract

The implantation and controlled release of growth factors can enhance the proliferation and differentiation of cells that promote new bone formation at defect sites. Therefore, chitosan polymer microspheres were prepared by the water-in-oil emulsion (W/O) method and solvent freeze-drying, using glutaraldehyde as an ionic crosslinker, along with the lyophilization of solvents, to microencapsulate growth factors, preventing denaturation. The microspheres were loaded with recombinant bone morphogenetic protein 2 (Rh-BMP-2). They were spherical in shape, with a rough surface ranging in particle size from 0.4 to 1.6 μm. The yield percentage with respect to the polymer was 70% and the BMP-2 load was regulated by the initial protein dose. BMP-2 release experiments were performed for 7 days in PBS solutions at pH 4 and 7.4. The results showed that the protein release rate was only 2% lower at pH 7.4. BMP-2/chitosan microspheres were compatible with the MG-63 cell line (ATCC®CRL-1427™Homo sapiens bone osteosarcoma) and could be considered drug delivery vehicles in bone tissue engineering applications.

Keywords

biomaterialbonefreeze dryingpolymer
Type
Articles
Information
MRS Advances , Volume 4 , Issue 59-60: International Materials Research Congress XXVIII , 2019 , pp. 3259 - 3267
Copyright
Copyright © Materials Research Society 2019 

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