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Investigation of Normal Cell and Cancer Cell Attachment and the Effects of Ganoderma Lucidum Using an Electric Impedance Sensing Technique

Published online by Cambridge University Press:  20 July 2020

Steffi Shi Qing Kong ,
Alejandra Martinez  and
Maddy Behravan
Steffi Shi Qing Kong
Affiliation:
Converse College, Biology, Chemistry, and Physics Department, 580 East Main Street, Spartanburg, SC29302
Alejandra Martinez
Affiliation:
Converse College, Biology, Chemistry, and Physics Department, 580 East Main Street, Spartanburg, SC29302
Maddy Behravan
Affiliation:
Converse College, Biology, Chemistry, and Physics Department, 580 East Main Street, Spartanburg, SC29302
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Abstract

This research introduces an application of an electric impedance sensing technique to investigate cell attachment of normal epithelial cells (HaCAT) and cancerous cells (A431) before and after addition of Ganoderma Lucidum (reishi). In this study, an impedance sensing system is used to measure and characterize real-time changes in electric impedance (resistance and capacitance) with respect to an alternating current (AC) applied to HaCAT and A431 cell colonies. The impedance data is related to the properties of cell spreading, attachment, and delamination. The effect of reishi at dosages of 0.005, 0.01, and 0.02 mg/ml on these cellular properties was inferred from impedance data. The initial impedance data show that resistance is greater for A431 cells than HaCAT cells and that capacitance for A431 cells is less than the capacitance for HaCAT cells. Further, the data shows the resistance for HaCAT cells and for A431 cells increases with time, and the capacitance for both decreases with time. The impedance data analysis shows that reishi plays no role in altering the impedance of the cellular matrix. At most, reishi serves as a lubricant to allow partial detachment and reattachment of HaCAT cell-to-cell bonds, thus reordering (< entropy) the cellular matrix. This effect is not seen in A431 cell colonies.

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Articles
Information
MRS Advances , Volume 5 , Issue 45: Soft Materials and Biomaterials , 2020 , pp. 2341 - 2348
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Copyright
Copyright © Materials Research Society 2020

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