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The Effects of Graphene Oxide and Partially Reduced Graphene Oxide on Staphylococcus aureus, Dermal Fibroblasts, and Keratinocytes

Published online by Cambridge University Press:  01 March 2018

Rebecca Isseroff ,
Roshan Reddy ,
Nicholas Williams ,
Jerry Reyes ,
Clement Marmorat ,
Marcia Simon ,
Fan Yang ,
Stephen Walker  and
Miriam Rafailovich
Rebecca Isseroff*
Affiliation:
Dept. of Materials Science and Chemical Engineering, Stony Brook University, Stony Brook, NY, 11794USA Lawrence High School, Cedarhurst, NY11516USA
Roshan Reddy
Affiliation:
Lawrence High School, Cedarhurst, NY11516USA
Nicholas Williams
Affiliation:
Lawrence High School, Cedarhurst, NY11516USA
Jerry Reyes
Affiliation:
Lawrence High School, Cedarhurst, NY11516USA
Clement Marmorat
Affiliation:
Dept. of Materials Science and Chemical Engineering, Stony Brook University, Stony Brook, NY, 11794USA
Marcia Simon
Affiliation:
Stony Brook School of Dental Medicine, Stony Brook, NY, 11794USA
Fan Yang
Affiliation:
Dept. of Materials Science and Chemical Engineering, Stony Brook University, Stony Brook, NY, 11794USA
Stephen Walker
Affiliation:
Stony Brook School of Dental Medicine, Stony Brook, NY, 11794USA
Miriam Rafailovich
Affiliation:
Dept. of Materials Science and Chemical Engineering, Stony Brook University, Stony Brook, NY, 11794USA
*
*(Email: [email protected])
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Abstract

Graphene and graphene oxide are being investigated for use in drug delivery systems, bioimaging, and antimicrobial applications. However, their effects, if any, on healthy cells need to be established before they can be deemed safe for therapeutic use. This research tested whether graphene oxide (GO) and/or partially reduced graphene oxide (pRGO) exhibit antimicrobial properties on Staphylococcus aureus; and also examined the growth and proliferation of dermal fibroblasts and keratinocytes in media modified with graphene oxide or partially reduced graphene oxide. Staphylococcus aureus was able to proliferate in both GO and pRGO- modified growth media as well as on gelatin made with GO and pRGO solutions. Both GO and pRGO increased dermal fibroblast doubling time and displayed lower cell counts compared to the control, with pRGO exerting a more pronounced effect than GO. After 4 days of keratinocyte incubation, GO and pRGO showed cell counts 75-80% less than the control. Cell counts of test samples dropped even lower by day 5 while the control cell count increased, suggesting that more investigation into the properties as well as the safety of graphene and its derivatives needs to be done before it is implemented for medical applications.

Keywords

biologicaladditivesbiomedical
Type
Articles
Information
MRS Advances , Volume 3 , Issue 13: Broader Impact , 2018 , pp. 679 - 689
Copyright
Copyright © Materials Research Society 2018 

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References

REFERENCES

Shen, H., Zhang, L., Liu, M., and Zhang, Z., Theranostics 2, 283 (2012).Google Scholar
Barua, S., Thakur, S., Aidew, L., Buragohain, A.K., Chattopadhyay, P., and Karak, N., RSC Advances 4, 9777 (2014).Google Scholar
He, J., Zhu, X., Qi, Z., Wang, C., Mao, X., Zhu, C., He, Z., Li, M., and Tang, Z., ACS Applied Materials & Interfaces 7, 5605 (2015).Google Scholar
Liu, S., Zeng, T.H., Hofmann, M., Burcombe, E., Wei, J., Jiang, R., Kong, J., and Chen, Y., ACS Nano 5, 6971 (2011).Google Scholar
Hummers, W.S. and Offeman, R.E., Journal of the American Chemical Society 80, 1339 (1958).Google Scholar
Isseroff, R., Akhavan, B., Pan, C., He, H.S., Sokolov, J., and Rafailovich, M., MRS Proceedings 1658, (2014).Google Scholar
Patel, S.C., Lee, S., Lalwani, G., Suhrland, C., Chowdhury, S.M., and Sitharaman, B., Therapeutic Delivery 7, 101 (2016).Google Scholar
Li, Z., Wang, Y., Du, D., Tang, Z., Wang, J., and Lin, Y., 2011 11th IEEE International Conference on Nanotechnology (2011).Google Scholar