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Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide

Published online by Cambridge University Press:  08 November 2019

Elani J. Cabrera-Vega Open the ORCID record for Elani J. Cabrera-Vega [Opens in a new window] ,
Georgina Alarcón-Ángeles ,
Martín Gómez Hernández ,
Gonzalo Campillo-Alvarado Open the ORCID record for Gonzalo Campillo-Alvarado [Opens in a new window]  and
Marcela Hurtado Y De la Peña
Elani J. Cabrera-Vega
Affiliation:
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Xochimilco, México, D.F.04960, México.
Georgina Alarcón-Ángeles
Affiliation:
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Xochimilco, México, D.F.04960, México.
Martín Gómez Hernández
Affiliation:
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Xochimilco, México, D.F.04960, México.
Gonzalo Campillo-Alvarado
Affiliation:
Department of Chemistry, University of Iowa, Iowa City, Iowa52242, United States.
Marcela Hurtado Y De la Peña*
Affiliation:
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Xochimilco, México, D.F.04960, México.
*
*(Email: [email protected])
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Abstract

We describe the use of immobilized deoxyribonucleic acid (DNA) in a silica matrix as a biorecognition agent for the detection of albendazole sulfoxide (ASU), the primary metabolite of albendazole and a suspected teratogenic and embryotoxic agent. The biomaterial (DNA-containing gel) was synthesized by physical entrapment of salmon sperm in an inorganic silicate matrix by the sol-gel method. Functionality of the DNA-containing gel was evaluated by comparative offline frontal chromatography followed by HPLC analysis of ASU and caffeine (CAF, control) using DNA-containing gel and DNA-free gel. The DNA-containing gel showed relatively high specific retention for ASU, while CAF showed no retention using frontal analysis. We anticipate that the DNA-containing gel can be implemented to identify the interactions of DNA with other active pharmaceutical ingredients (APIs) and their metabolites in a readily available, sensitive and selective frontal chromatography experiment.

Keywords

biomaterialsol-gelfunctional
Type
Articles
Information
MRS Advances , Volume 4 , Issue 64: International Materials Research Congress XXVIII , 2019 , pp. 3537 - 3543
Copyright
Copyright © Materials Research Society 2019 

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References

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