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Comparison of DNAzyme activity for the development of an immobilized heme sensor

Published online by Cambridge University Press:  05 January 2018

Natalie Hughes ,
Nancy Nguyen ,
Deanna-Kaye Daley ,
Justin Grennell ,
Amira Gee  and
Mehnaaz F. Ali
Natalie Hughes
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Nancy Nguyen
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Deanna-Kaye Daley
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Justin Grennell
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Amira Gee
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Mehnaaz F. Ali*
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
*
*(Email: [email protected])
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Abstract

Point-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

Keywords

devicesopticalsensor
Type
Articles
Information
MRS Advances , Volume 3 , Issue 26: Biomaterials and Soft Materials , 2018 , pp. 1491 - 1496
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Copyright
Copyright © Materials Research Society 2017 

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References

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