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Biopatterning of Keratinocytes in Aqueous Two-Phase Systems as a Potential Tool for Skin Tissue Engineering

Published online by Cambridge University Press:  15 May 2017

Rishima Agarwal ,
Kristin Robin Ko ,
Paul F. Gratzer  and
John P. Frampton
Rishima Agarwal*
Affiliation:
School of Biomedical Engineering, Dalhousie University, Halifax, NS , B3H 4R2, Canada
Kristin Robin Ko
Affiliation:
School of Biomedical Engineering, Dalhousie University, Halifax, NS , B3H 4R2, Canada
Paul F. Gratzer
Affiliation:
School of Biomedical Engineering, Dalhousie University, Halifax, NS , B3H 4R2, Canada
John P. Frampton
Affiliation:
School of Biomedical Engineering, Dalhousie University, Halifax, NS , B3H 4R2, Canada
*
*(Email: [email protected])
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Abstract

Extrusion-based bioprinting (EBP) is limited by loss of pattern fidelity when printing on wet substrates. This can be overcome using aqueous two-phase systems (ATPSs) as novel ink formulations for EBP. In this study, optimal concentrations of ATPS “inks” were determined and used to pattern human epidermal keratinocyte (HEK001) colonies on a wet substrate for promoting epidermal growth. Four equilibrated and non-equilibrated ATPS formulations were tested for stable ATPS formation and uniform cell patterning. We identified an optimal formulation that produced stable droplets on a standard tissue culture plate coated with PEG. This process was also tested on an acellular dermal matrix (DermGENTM ) to evaluate biopattern fidelity on a tissue matrix. Cell proliferation and formation of adherens junctions between cells were analyzed by immunocytochemistry. Non-equilibrated 5.0% PEG and 5.0% DEX solutions formed tighter colonies than equilibrated solutions containing identical total polymer concentrations. Cells patterned in colonies displayed higher cell viability and increased formation of E-cadherin junctions compared to non-patterned cells. Finally, when the cells were patterned on DermGENTM , discrete cell colonies were observed. This suggests that ATPS EBP holds promise for biopatterning epidermal keratinocyte cells to improve skin tissue engineering.

Keywords

polymerphase equilibriacellular (material form)
Type
Articles
Information
MRS Advances , Volume 2 , Issue 45: Soft Materials and Biomaterials , 2017 , pp. 2443 - 2449
Copyright
Copyright © Materials Research Society 2017 

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