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Iron-Doped Apatite Nanoparticles Delivered via Electrospun Fiber Mesh for Maximized Bacterial Killing by Bacteriophage

Published online by Cambridge University Press:  07 June 2017

Jessica M. Andriolo ,
Gary F. Wyss ,
John P. Murphy ,
Marisa L. Pedulla ,
M. Katie Hailer  and
Jack L. Skinner
Jessica M. Andriolo*
Affiliation:
Biomedical Engineering, IIP, University of Montana, Missoula, MT 59812
Gary F. Wyss
Affiliation:
CAMP, Montana Tech, Butte, MT 59701
John P. Murphy
Affiliation:
Materials Science, Montana Tech, Butte, MT 59701
Marisa L. Pedulla
Affiliation:
Biological Sciences, Montana Tech, Butte, MT 59701
M. Katie Hailer
Affiliation:
Chemistry and Geochemistry, Montana Tech, Butte, MT 59701
Jack L. Skinner
Affiliation:
Mechanical Engineering, Montana Tech, Butte, MT 59701
*
*(Email: [email protected])
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Abstract

According to the Centers for Disease Control (CDC) and prevention, at least 2 million people in the United States become infected with antibiotic-resistant bacteria, and at least 23,000 people die each year as a direct result of those infections. One alternative to traditional antibiotics is bacteriophage (phage) therapy. Phage therapy utilizes bacteria-specific viruses to infect and kill bacteria cells. The specificity of these viruses is beneficial in that phage used for therapeutic purposes do not harm the human microbiota, nor do phage infect eukaryotic cells. It has been discovered that iron-doped apatite nanoparticles (IDANPs) significantly enhance phage killing of bacteria cells. The biocompatibility of apatite, coupled with its effectiveness as an adjuvant to enhance an alternative antibiotic therapy, makes it of interest for medical applications. Previously, researchers have encased phage in a microfluidic channel in coaxially electrospun fibers, allowing phage to remain viable after several weeks storage at 4 °C. Here, we have constructed a polymer fiber layer using electrospinning (ES) for delivery of IDANP adjuvants to compliment phage treatment delivery fibers. The IDANP delivery layer constructed is composed of polyethylene oxide (PEO) doped with the nanoparticles. When compared to media-only and IDANP-only controls, results show IDANPs delivered through a PEO fiber mesh remain effective at enhancement of phage infectivity.

Keywords

nanoscalebiomedicalfiber
Type
Articles
Information
MRS Advances , Volume 2 , Issue 46: Soft Materials and Biomaterials , 2017 , pp. 2465 - 2470
Copyright
Copyright © Materials Research Society 2017 

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