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In-situ TEM observation of rock salt crystal precipitation in liposome

Published online by Cambridge University Press:  21 April 2016

Haruka Ai ,
Naoto Moriya ,
Takuji Ube ,
Takashi Harumoto ,
Yoshihiro Arai ,
Kazuyoshi Murata  and
Takashi Ishiguro
Haruka Ai
Affiliation:
Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijyuku, Katsushika-ku, Tokyo 125-8585, Japan
Naoto Moriya
Affiliation:
Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijyuku, Katsushika-ku, Tokyo 125-8585, Japan
Takuji Ube
Affiliation:
Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijyuku, Katsushika-ku, Tokyo 125-8585, Japan
Takashi Harumoto
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, 2-12-1-S8-6 Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Yoshihiro Arai
Affiliation:
Terabase Inc., 3-5-1-102 Hanenishi, Okazaki, Aichi, 444-0838, Japan
Kazuyoshi Murata
Affiliation:
National Institute for Physiological Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan
Takashi Ishiguro*
Affiliation:
Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijyuku, Katsushika-ku, Tokyo 125-8585, Japan
*
*(Email: [email protected])
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Abstract

Liposome reinforced by adding cholesterol was synthesized using the dipalmitoyl-phosphatidylcholine (DPPC). Phase stability of the phospholipid bilayers was examined by FT-IR spectroscopy. The reinforced liposomes filled with ultrapure water or physiological saline were observed by the conventional TEM equipped with a mass spectrometer. It was confirmed that the liposomes filled with water or saline solution were stable in the vacuum at room temperature. However, during electron irradiation, water molecules escaped gradually, and a single crystal of rock salt precipitated in the liposome.

Keywords

transmission electron microscopy (TEM)cellular (material form)biomaterial
Type
Articles
Information
MRS Advances , Volume 1 , Issue 25: Theory, Characterization, and Modeling , 2016 , pp. 1871 - 1876
Copyright
Copyright © Materials Research Society 2016 

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References

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