Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-23T03:16:06.909Z Has data issue: false hasContentIssue false
  • English
  • Français

A phylogenetic study of the Micarea prasina group shows that Micarea micrococca includes three distinct lineages

Published online by Cambridge University Press:  26 November 2009

Paweł CZARNOTA  and
Beata GUZOW-KRZEMIŃSKA
Paweł CZARNOTA
Affiliation:
University of Rzeszów, Faculty of Biology and Agriculture, Department of Agroecology, ul. Ćwiklińskiej 2, PL-35-601 Rzeszów, Poland; Scientific Laboratory of the Gorce National Park, Poręba Wielka 590, PL-34-735 Niedźwiedź. Email: [email protected]
Beata GUZOW-KRZEMIŃSKA
Affiliation:
University of Gdańsk, Faculty of Biology, Department of Molecular Biology, ul. Kładki 24, PL-80-822 Gdańsk, Poland.
Get access Rights & Permissions [Opens in a new window]

Abstract

The phylogeny of the Micarea prasina group was investigated using mitochondrial small subunit ribosomal DNA sequences from 14 taxa representing this group, four other members of the genus Micarea, and Psilolechia lucida as an outgroup. A total of 31 new mtSSU rDNA sequences were generated, including 10 from the M. micrococca complex. Bayesian, maximum parsimony (MP) and maximum likelihood (ML) methods were used to analyse the data. The results show that M. micrococca is not monophyletic and forms three strongly supported lineages: 1) M. micrococca s. str., 2) M. byssacea (Th. Fr.) Czarnota, Guzow-Krzemińska & Coppins comb. nov., and 3) a putative taxon that requires further studies. Micarea viridileprosa is a sister species to M. micrococca s. str. and the recently described M. nowakii is a sister species to M. prasina s. str. The placement of M. tomentosa within the M. prasina group is confirmed. Micarea hedlundii appears to be more closely related to the M. micrococca complex than M. prasina s. str. Descriptions, illustrations, taxonomic remarks, distribution and habitat data for M. micrococca s. str. and M. byssacea are provided. A lectotype for Biatora byssacea Hampe non Zwackh and a neotype for Catillaria prasina β [var.] byssacea are selected.

Keywords

lichensMicarea byssaceamtSSU rDNAphylogenysecondary metabolitestaxonomy
Type
Research Article
Information
The Lichenologist , Volume 42 , Issue 1 , January 2010 , pp. 7 - 21
Copyright
Copyright © British Lichen Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.CrossRefGoogle ScholarPubMed
Andersen, H. L. (2004) Phylogeny and classification of Micarea. Ph.D. thesis, University of Bergen, Norway.Google Scholar
Andersen, H. L. & Ekman, S. (2005) Disintegration of the Micareaceae (lichenized Ascomycota): a molecular phylogeny based on mitochondrial rDNA sequences. Mycological Research 109: 21–30.CrossRefGoogle ScholarPubMed
Articus, K., Mattsson, J.-E., Tibell, L., Grube, M. & Wedin, M. (2002) Ribosomal DNA and β-tubulin data do not support the separation of the lichens Usnea florida and U. subfloridana as distinct species. Mycological Research 106: 412–418.CrossRefGoogle Scholar
Buschbom, J. & Mueller, G. M. (2006) Testing “species pair” hypotheses: evolutionary process in the lichen-forming species complex: Porpidia flavocoerulescens and Porpidia melinodes. Molecular Biology and Evolution 23: 574–586.CrossRefGoogle ScholarPubMed
Coppins, B. J. (1983) A taxonomic study of the lichen genus Micarea in Europe. Bulletin of the British Museum (Natural History), Botany series 11: 17–214.Google Scholar
Coppins, B. J. (1992) Micarea Fr. (1825) In The Lichen Flora of Great Britain and Ireland (Purvis, O. W., Coppins, B. J., Hawksworth, D. L., James, P. W. & More, D. M., eds): 371–384. London: Natural History Museum Publications.Google Scholar
Coppins, B. J. (2002) Checklist of Lichens of Great Britain and Ireland. London: British Lichen Society.Google Scholar
Coppins, B. J. & Tønsberg, T. (2001) A new xanthone – containing Micarea from Northwest Europe and Pacific Northwest of North America. Lichenologist 33: 93–96.CrossRefGoogle Scholar
Czarnota, P. (2007) The lichen genus Micarea (Lecanorales, Ascomycota) in Poland. Polish Botanical Studies 23: 1–199.Google Scholar
Czarnota, P. & Coppins, B. J. (2007) Contribution to the knowledge of rare Bacidia s. lat. (Lecanorales, lichenized Ascomycetes) from Central Europe including a new, pallid forma of Bacidia hemipolia. Nova Hedwigia 85: 503–513.CrossRefGoogle Scholar
Divakar, P. K., Molina, M. C., Lumbsch, H. T. & Crespo, A. (2005) Parmelia barrenoae, a new lichen species related to Parmelia sulcata (Parmeliaceae) based on molecular and morphological data. Lichenologist 37: 37–46.CrossRefGoogle Scholar
Divakar, P. K., Crespo, A., Blanco, O. & Lumbsch, H. T. (2006) Phylogenetic significance of morphological characters in the tropical Hypotrachyna clade of parmelioid lichens (Parmeliaceae, Ascomycota). Molecular Phylogenetics and Evolution 40: 448–458.CrossRefGoogle ScholarPubMed
Elix, J. A., Jones, A. J., Lajide, L., Coppins, B. J. & James, P. W. (1984) Two new diphenyl ethers and a new depside from the lichen Micarea prasina Fr. Australian Journal of Chemistry 37: 2349–2364.CrossRefGoogle Scholar
Eriksson, O. E., Baral, H.-O., Currah, R. S., Hansen, K., Kurtzman, C. P., Rambold, G. & Laessøe, T. (2004) Outline of Ascomycota. Myconet 10: 1–99.Google Scholar
Fries, T. M. (1874) Lichenographia Scandinavica sive dispositio lichenum in Dania, Suecia, Norvegia, Fennia, Lapponia Rossica hactus collectorum. Vol. I Archilichenes discocarpos continens. Pars II: 325–639. Upsaliae.Google Scholar
Galtier, N., Gouy, M. & Gautier, C. (1996) SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Computational Applied Biosciences 12: 543–548.Google ScholarPubMed
Goffinet, B. & Miądlikowska, J. (1999) Peltigera phyllidiosa (Peltigeraceae, Ascomycotina), a new species from the Southern Appalachians corroborated by ITS sequences. Lichenologist 31: 247–256.Google Scholar
Grube, M. & Kroken, S. (2000) Molecular approaches and the concept of species and species complexes in lichenized fungi. Mycological Research 104: 1284–1294.CrossRefGoogle Scholar
Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52: 696–704.CrossRefGoogle ScholarPubMed
Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O. (2005) PHYML Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Research 33: W557–W559.CrossRefGoogle ScholarPubMed
Guzow-Krzemińska, B. & Węgrzyn, G. (2000) Potential use of restriction analysis of PCR-amplified DNA fragments in taxonomy of lichens. Mycotaxon 76: 305–313.Google Scholar
Hafellner, J. (1984) Studien in Richtung einer natürlicheren Gliederung der Sammelfamilien Lecanoraceae und Lecideaceae. Beiheft zur Nova Hedwigia 79: 241–371.Google Scholar
Hedlund, J. T. (1892) Kritische Bemerkungen über einige Arten der Flechtengattungen Lecanora (Ach.), Lecidea (Ach.) und Micarea (Fr.). Bihang till Kungliga Svenska Vetenskaps-Akademiens Handligar. III 18(3): 1–104.Google Scholar
Huelsenbeck, J. P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754–755.CrossRefGoogle Scholar
Kroken, S. & Taylor, J. W. (2001) A gene genealogical approach to recognize phylogenetic species boundaries in the lichenized fungus Letharia. Mycologia 93: 38–53.CrossRefGoogle Scholar
Lumbsch, H. T., Nelsen, M.P. & Lücking, R. (2008) The phylogenetic position of Haematommataceae (Lecanorales, Ascomycota), with the notes on secondary chemistry and species delimitation. Nova Hedwigia 86: 105–114.CrossRefGoogle Scholar
Meyer, B. & Printzen, C. (2000) Proposal for a standarized nomenclature and characterization of insoluble lichen pigments. Lichenologist 32: 571–583.CrossRefGoogle Scholar
Molina, M. C., Crespo, A., Blanco, O., Lumbsch, H. T. & Hawksworth, D. L. (2004) Phylogenetic relationships and species concepts in Parmelia s. str. (Parmeliaceae) inferred from nuclear ITS rDNA and β-tubulin sequences. Lichenologist 36: 37–54.CrossRefGoogle Scholar
Nelsen, M. P. & Gargas, A. (2008) Phylogenetic distribution and evolution of secondary metabolities in the lichenized fungal genus Lepraria (Lecanorales: Stereocaulaceae). Nova Hedwigia 86: 115–131.CrossRefGoogle Scholar
Nelsen, M. P. & Gargas, A. (2009) Assesing clonality and chemotype monophyly in Thamnolia (Icmadophilaceae). Bryologist 112: 42–53.CrossRefGoogle Scholar
Nordin, A., Tibell, L. & Owe-Larsson, B. (2007) A preliminary phylogeny of Aspicilia in relation to morphological and secondary product variation. Bibliotheca Lichenologica 96: 247–266.Google Scholar
Orange, A., James, P. W. & White, F. J. (2001) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.Google Scholar
Page, R. D. M. (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–358.Google ScholarPubMed
Posada, D. & Crandall, K. A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818.CrossRefGoogle ScholarPubMed
Rambaut, A. & Drummond, A. J. (2007) Tracer v1.4 (http://beast.bio.ed.ac.uk/Tracer)Google Scholar
Ronquist, F. & Huelsenbeck, J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.CrossRefGoogle ScholarPubMed
Shimodaira, H. & Hasegawa, M. (1999) Mulltiple comparisons of log-likelihood with applications to phylogenetic inference. Molecular Biology and Evolution 16: 1114–1116.CrossRefGoogle Scholar
Stocker-Wörgötter, E., ElixJ. A. & Grube, M. J. A. & Grube, M. (2004) Secondary chemistry of lichen-forming fungi: chemosyndromic variation and DNA-analyses of cultures and chemotypes in the Ramalina farinacea complex. Bryologist 107: 152–162.CrossRefGoogle Scholar
Swofford, D. L. (2001) PAUP*: Phylogenetic Analysis Using Parsimony (and Other Methods). Version 4. Sunderland: Sinauer Associates.Google Scholar
Tehler, A. & Källersjö, M. (2001) Parmeliopsis ambigua and P. hyperopta (Parmeliaceae): species or chemotypes? Lichenologist 33: 403–408.CrossRefGoogle Scholar
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876–4882.CrossRefGoogle Scholar
Tønsberg, T. (1992) The sorediate and isidiate, corticolous, crustose lichens in Norway. Sommerfeltia 14: 1–133.CrossRefGoogle Scholar
Vainio, E. A. (1899) Lichenes e Caucaso et in peninsula Taurica annis 1884–1885 ab H. Lojka et M. a Déchy collecti. Természetrajzi Füzetek 22: 269–343.Google Scholar
van den Boom, P. P. G. & Coppins, B. J. (2001) Micarea viridileprosa sp. nov., an overlooked lichen species from Western Europe. Lichenologist 33: 87–91.CrossRefGoogle Scholar
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31: 511–516.CrossRefGoogle Scholar