Introduction
The genus Malmidea Kalb et al. and the family Malmideaceae were together established by Kalb et al. (Reference Kalb, Rivas Plata, Lücking and Lumbsch2011) to resolve the phylogenetic position of the Lecidea piperis and Lecanora granifera groups. At present the family Malmideaceae contains a total of nine genera: Australidea Kantvilas et al.; Cheiromycina B. Sutton; Crustospathula Aptroot; Kalbionora Sodamuk et al.; Malmidea Kalb et al.; Multisporidea Kalb & Aptroot; Savoronala Ertz et al.; Sprucidea M. Cáceres et al.; Zhurbenkoa Flakus et al. Of these, Zhurbenkoa is lichenicolous while the others are lichenized fungi (Sutton & Muhr Reference Sutton and Muhr1986; Aptroot Reference Aptroot1998; Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011; Ertz et al. Reference Ertz, Fischer, Killmann, Razafindrahaja and Sérusiaux2013; Breuss & Lücking Reference Breuss and Lücking2015; Cáceres et al. Reference Cáceres, Aptroot, Mendonça, Santos and Lücking2017; Muggia et al. Reference Muggia, Mancinelli, Tønsberg, Jablonska, Kukwa and Palice2017; Sodamuk et al. Reference Sodamuk, Boonpragob, Mongkolsuk, Tehler, Leavitt and Lumbsch2017; Flakus et al. Reference Flakus, Etayo, Pérez-Ortega, Kukwa, Palice and Flakus2019; Kalb & Aptroot Reference Kalb and Aptroot2021; Kantvilas et al. Reference Kantvilas, Wedin and Svensson2021). The genus Malmidea is characterized by having a mostly corticolous habitat, a crustose, smooth to verrucose thallus, often formed by goniocysts, pigmentation often present in the medulla, an excipulum usually with medullary hyphae in layers or in chambers incrusted with opaque to pigmented hydrophobic granules, asci without a distinct tubular structure in the tholus, simple, hyaline ascospores (1–2–)4–8 per ascus, the wall halonate and evenly thickened or thickened at the ends, and atranorin often present (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011). A world key to the known species of Malmidea was provided by Breuss & Lücking (Reference Breuss and Lücking2015) which included 50 species. Prior to the present study, 72 species of Malmidea were known worldwide, of which 17 have been reported from India (Singh & Pinokiyo Reference Singh and Pinokiyo2014; Sinha et al. Reference Sinha, Gupta, Kar and Joseph2015; Joseph et al. Reference Joseph, Nayaka, Randive and Upreti2018; Gogoi et al. Reference Gogoi, Joseph, Choudhury, Nayaka and Yasmin2020; Islary et al. Reference Islary, Biswas, Nayaka, Joseph, Upreti, Basumatary and Daimari2023). Our recent lichen collections from Western Himalaya and a detailed study of specimens preserved in the herbarium LWG resulted in several novel or interesting species that are described herein.
Materials and Methods
The specimens reported in the present study were recently collected from the Western Himalayas, en route to Sunderdhunga glacier spanning an altitude of 2000–4000 m. In addition, identified and unidentified specimens of Malmidea preserved in the herbarium LWG of the CSIR-National Botanical Research Institute, Lucknow were re-examined. Morphological analysis and image capture were carried out using a Leica S9i stereo zoom microscope with an integrated digital camera. Anatomical characters were analyzed using Leica S8 APO and Leica DM2500 microscopes. For the spot tests, the reagents 10% KOH (K) and p-Phenylenediamine (P) were used. The presence of secondary metabolites was detected by performing thin-layer chromatography (TLC) in solvent system A following Orange et al. (Reference Orange, James and White2001).
Results and Discussion
This study resulted in the description of 10 species of Malmidea new to science and seven new records for the Indian lichen biota. As a result, the global count of Malmidea has increased to 82 species, with 34 for India. The re-examination of specimens that were previously identified as Lecidea granifera (Ach.) Vain. yielded several intriguing species. Notable among them are species with a thalline excipulum. Previously only M. duplomarginata (Papong & Kalb) Kalb & Papong was known to have a thalline excipulum, but here we add three more species, viz. M. incrassatispora, M. kalbii and M. palghatensis. Furthermore, thickened ascospore cell wall ends were observed in M. incrassatispora, a species-specific characteristic previously known in only two other species, M. incrassata Kalb and M. reunionis Kalb. Detailed descriptions of the new species are provided and the world key by Breuss & Lücking (Reference Breuss and Lücking2015) has also been updated by providing the key couplets of all the Malmidea species.
The New Species
Malmidea glabromarginata R. Adhikari, S. Joseph & Nayaka sp. nov.
MycoBank No.: MB 852244
Similar to Malmidea badimioides (M. Cáceres & Lücking) M. Cáceres & Kalb but differs in having smaller ascospores, 10–15 × 6–9 μm (vs 15–20 × 6–10 μm).
Type: India, Odisha, Kandhamal District, Raikia, near Mandasaru valley, 19°58ʹ27ʺN, 84°14ʹ27ʺE, 726 m, 27 December 2019, R. R. Paul 19-047442 (LWG—holotype).
(Fig. 1)
Figure 1. Habit of Malmidea glabromarginata (holotype LWG-19-047442). Scale = 1 mm. In colour online.
Thallus crustose, corticolous, greenish grey, continuous, finely verrucose; verrucae small and sparse, up to 0.1 mm diam. and 0.1 mm high; isidia and soredia absent. Prothallus indistinct. Medulla of thallus and verrucae white.
Apothecia round, sessile, 0.4–0.7 mm diam.; disc plane to slightly concave, orange-brown, margin granifera-type, distinctly white, 80–100 μm thick, smooth, continuous. Thalline exciple absent. Proper exciple 70–120 μm wide, externally hyaline filled with a continuous layer of medullary hyphae incrusted with opaque to whitish granules which dissolve in K with a lemon yellow reaction, internally pale to brownish, ectal excipulum poorly developed. Epihymenium indistinct. Hymenium hyaline, 60–70 μm high. Subhymenium hyaline, 20–25 μm high. Hypothecium 60–70 μm high, dark brownish, K−. Apothecial base below hypothecium 70–80 μm high with a continuous layer of medullary hyphae and greyish granules. Asci 45–65 × 10–17 μm, 4–8-spored. Ascospores simple, hyaline, ellipsoid, 10–14 (–15) × 6–8 (–9) μm, wall evenly thickened, halonate (1 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus and verrucae K+ bright yellow, P−. TLC: no secondary metabolites detected.
Etymology
The epithet ‘glabromarginata’ refers to the smooth apothecial margin of the species.
Remarks
This species is characterized by having a finely verrucose thallus, small-sized apothecia (up to 0.7 mm diam.) with orange-brown coloured discs and distinctly white margins. It is close to Malmidea badimioides and M. psychotrioides (Kalb & Lücking) Kalb et al. Malmidea badimioides has larger ascospores (15–20 × 6–10 μm), reddish brown apothecial discs with margins becoming granular with age and an orange-coloured hypothecium (Breuss & Lücking Reference Breuss and Lücking2015; Kalb Reference Kalb2021). Malmidea psychotrioides has mostly 4-spored asci, a well-developed ectal excipulum, smooth to crenulate apothecial margins and a foliicolous habit (Lücking & Kalb Reference Lücking and Kalb2000).
Additional specimens examined
India: Odisha: Kandhamal District, Raikia, near Mandasaru valley, 19°58ʹ27ʺN, 84°14ʹ27ʺE, 726 m, 2019, R. R. Paul 19-047443 & 19-047444 (LWG).
Malmidea globosa R. Adhikari & Nayaka sp. nov.
MycoBank No.: MB 852245
Similar to Malmidea papillosa Weerakoon & Aptroot and M. allopapillosa Kalb but differs in having smaller ascospores, 9–15 × 6–9 μm.
Type: India, Mizoram, Champhai District, Murlen National Park, 2122 m, 24 September 2014, A. R. Logesh & Chinlampianga 14-021051/A (LWG—holotype).
(Fig. 2)
Figure 2. Habit of Malmidea globosa (holotype LWG-14-021051/A). Scale = 0.5 mm. In colour online.
Thallus crustose, corticolous, continuous, greenish grey, densely verrucose; verrucae confluent, almost spherical, constricted at base, 0.1–0.15 mm diam. and c. 0.1 mm high; isidia and soredia absent. Prothallus indistinct. Medulla of the thallus white, medulla of verrucae cream to yellowish.
Apothecia sessile, round to irregular, 0.4–0.5 mm diam.; disc plane to slightly convex, brown to dark brown; margin of piperis-type, thin, 30–40 μm, level with disc, concolorous with the thallus or paler. Thalline exciple absent. Proper exciple 40–95 μm wide, hyaline externally, light to dark brown inside, lacking hydrophobic granules. Epihymenium indistinct. Hymenium 35–50 μm high, hyaline. Subhymenium 16–20 um high, hyaline, piperis-type. Hypothecium 50–65 μm high, pale brown to dark brown, K−. Apothecial base below hypothecium 70–80 μm high, hyaline to pale. Asci 35–45 × 11–18 μm, mostly 8-spored. Ascospores simple, hyaline, ellipsoid, 9–13(–15) × 6–8(–9) μm, wall equally thickened, halonate (1 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae cream to yellowish, K+ orange-yellow, P−. TLC: atranorin (major) and unknown purple spots at R f class 5 and just below 7 in solvent A.
Etymology
The species epithet ‘globosa’ refers to its globose verrucae.
Remarks
This species is characterized by having a strongly verrucose thallus with almost spherical warts, a piperis-type exciple and medulla of verrucae cream to yellowish. A combination of the verrucose thallus, pigments in the medulla and piperis-type apothecial margins, suggests this species resembles M. papillosa and M. allopapillosa. However, in both, the verrucae are not constricted at the base, and ascospores are larger (15–18 × 9–11 μm in M. papillosa and 14–17 × 8–10 in M. allopapillosa). The medulla of verrucae in M. allopapillosa is peach-coloured and also contains atranorin, and in M. papillosa the apothecial margin is darker than the disc (Weerakoon & Aptroot Reference Weerakoon and Aptroot2014; Kalb Reference Kalb2021). Malmidea amazonica (Redinger) Kalb et al. also has some resemblance, but the thallus medulla is orange-red turning purple in K (Breuss & Lücking Reference Breuss and Lücking2015).
Additional specimen examined
India: Mizoram: Champhai District, Murlen National Park, 2122 m, 2014, A. R. Logesh & Chinlampianga 14-021051/B (LWG).
Malmidea incrassatispora R. Adhikari, S. Joseph & Nayaka sp. nov.
MycoBank No.: MB 852246
Similar to Malmidea duplomarginata but differs in having ascospores with end wall thickenings and yellowish to faintly orange-yellow pigmented medulla of verrucae.
Type: India, Karnataka, Uttara Kannada, Castle Rock, Singargao cross, 25 February 2018, S. Nayaka, S. Joseph & S. Dudani 18-047441 (LWG—holotype).
(Fig. 3)
Figure 3. Habit of Malmidea incrassatispora (holotype LWG- 18-047441). A, thallus. B, ascospores with end wall thickenings. Scales: A = 1 mm; B = 10 μm. In colour online.
Thallus crustose, corticolous, greenish grey, verrucose; verrucae 0.1–0.14 mm diam. and 0.1 mm high, confluent; isidia and soredia absent. Prothallus indistinct. Medulla of thallus white to cream-coloured, medulla of verrucae faintly yellow to light orange-yellow.
Apothecia round, sessile, 0.4–0.8 mm diam.; disc orange-brown to dark reddish brown, plane to slightly concave; margin granifera-type, 130–170 μm thick, prominent, continuous to slightly granular. Thalline exciple hyaline, 30–40 μm thick, densely filled with algal cells. Proper exciple 110–140 μm wide, internally brownish, externally hyaline, filled with a continuous layer of medullary hyphae and creamish to pale hydrophobic granules, dissolving in K with a lemon yellow to greenish yellow reaction. Epihymenium pale to orange-brown, 10–15 μm high. Hymenium hyaline, 75–90 μm high. Subhymenium hyaline, 20–25 μm high. Hypothecium 70–85 μm high, dark brown, K−. Apothecial base below hypothecium 110–130 μm high, hyaline with a continuous layer of medullary hyphae with greyish to cream-coloured granules. Asci 80–90 × 15–22 μm, 3–6-spored (rarely 8). Ascospores simple, hyaline, ellipsoid, 20–24 (–27) × 10–13(–14) μm, wall not uniformly thickened, slightly to prominently thickened at ends, halonate (2–3 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ orange, P−. TLC: atranorin, unknown grey-brown spots at R f classes 5 and 6.
Etymology
The species epithet ‘incrassatispora’ refers to the ascospores having prominently thickened ends.
Remarks
This species is characterized by having a thalline excipulum and ascospores with distinct end wall thickenings. The presence of such ascospores is not common in this genus and is considered to be a prominent character in distinguishing species (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011, Reference Kalb, Buaruang, Mongkolsuk and Boonpragob2012; Breuss & Lücking Reference Breuss and Lücking2015). Currently Malmidea duplomarginata is the only known Malmidea species with a thalline excipulum but it has ascospores with evenly thickened walls and differs in the medulla of verrucae being cream to white-coloured, larger apothecia up to 1.5 mm diam., and up to 8-spored asci (Kalb et al. Reference Kalb, Buaruang, Papong and Boonpragob2009).
Additional specimens examined
India: Karnataka: Shimoga District, Sagar, Marur Village, 14°12ʹ47.2ʺN, 75°05ʹ45.7ʺE, 630 m, 2018, S. Nayaka, S. Joseph & S. Dudani 18–034389 (LWG); Uttara Kannada, towards Castle Rock Town, 2018, S. Nayaka, S. Joseph & S. Dudani 18-036458 (LWG). Madhya Pradesh: Hoshangabad District, Panchmarhi, Patharchatta, 762 m, 1973, S. R. Singh 73.54 (LWG). Mizoram: Champhai District, Murlen National Park, 2092 m, 2014, A. R. Logesh & Chinlampianga 14-031409 (LWG).
Malmidea kalbii R. Adhikari & Nayaka sp. nov.
MycoBank No.: MB 852247
Similar to Malmidea duplomarginata but differs in having smaller ascospores, 9–15 × 6–9 μm (vs 20–24 × 11–15 μm).
Type: India, Odisha, Kandhamal District, Raikia, near Mandasaru valley, 19°58ʹ27ʺN, 84°14ʹ27ʺE, 726 m, 27 December 2019, R. R. Paul 19-039538/B (LWG—holotype).
(Fig. 4)
Figure 4. Habit of Malmidea kalbii (holotype LWG- 19-039538/B). A, thallus. B, apothecium. C, apothecial section. Scales: A = 1 mm; B = 0.5 mm; C = 50 mm. In colour online.
Thallus crustose, corticolous, greenish grey, continuous to cracked, verrucose; verrucae not prominent; isidia and soredia absent. Prothallus whitish with grey margin. Medulla of thallus and verrucae white.
Apothecia round, sessile, 0.5–1.2 mm diam.; disc dark reddish brown to black, plane to slightly convex; margin granifera-type, concolorous with the thallus, 75–90 μm thick, not prominently raised, continuous. Thalline exciple 50–60 μm wide, densely filled with algal cells. Proper exciple 70–130 μm wide, filled with a continuous layer of medullary hyphae and greyish hydrophobic granules, dissolving in K with a pale to lemon yellow reaction. Epihymenium indistinct. Hymenium hyaline, 65–75 μm high. Subhymenium 20–25 μm high, hyaline to slightly pale. Hypothecium 65–90 μm high, orange-brown to dark brown, K−. Apothecial base below hypothecium 100–130 μm high, hyaline, filled with a continuous layer of medullary hyphae and greyish granules. Asci 60–70 × 10–15 μm, (4–)6–8-spored. Ascospores simple, hyaline, ellipsoid, 9–14(–15) × 6–8(–9) μm, wall uniformly thickened, halonate (1 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus and verrucae K+ bright yellow to light orange-yellow, P−. TLC: atranorin.
Etymology
This species is named in honour of Prof. Dr Klaus Kalb, a renowned German lichenologist.
Remarks
This species is characterized by having a thalline excipulum, dark brown to black apothecial discs and ascospores mostly < 15 μm in length. It is similar to M. duplomarginata, the only known Malmidea species with a thalline excipulum, and two other species (M. incrassatispora and M. palghatensis) described in the present study. Malmidea duplomarginata has larger ascospores, 20–24 × 11–15 μm, and plane to concave comparatively less dark apothecial discs (Kalb et al. Reference Kalb, Buaruang, Papong and Boonpragob2009). Malmidea incrassatispora and M. palghatensis also have larger ascospores (mostly > 15 μm), pigmented medulla of verrucae, less dark apothecial discs and lack a prothallus.
Additional specimen examined
India: Odisha: Mayurbhanj District, Lanjioghosara, 2019, D. K. Upreti & S. Pradhan CUTM-073 (LWG).
Malmidea lutea R. Adhikari, Ingle & Nayaka sp. nov.
MycoBank No.: MB 852248
Similar to Malmidea badimioides but differs in having beige-coloured apothecia (red-brown in M. badimioides), a hyaline hypothecium (orange in M. badimioides) and larger ascospores, 20–25 × 10–15 μm (vs 14–20 × 7–10 μm).
Type: India, Uttarakhand, Bageshwar District, en route to Jatoli from Khati Village, 30°08ʹ30.33ʺN, 79°55ʹ21.57ʺE, 2440 m, 19 June 2023, Komal K. Ingle & R. S. Adhikari 23-047445 (LWG—holotype).
(Fig. 5)
Figure 5. Habit of Malmidea lutea (holotype LWG- 23-047445). Scale = 2 mm. In colour online.
Thallus crustose, corticolous, greenish grey, thick, continuous, verrucose; verrucae 0.20–0.24 mm diam. and 0.1–0.15 mm high, confluent, hemispherical to irregular. Prothallus indistinct. Medulla of thallus and verrucae white to creamish.
Apothecia round, sessile, 0.4–0.9 mm diam., constricted at base; disc beige, plane to concave; margin granifera-type, pale, lighter than disc colour, 0.11–0.14 mm thick, slightly prominent, smooth, continuous to slightly papillate towards maturity. Thalline exciple absent. Proper exciple 80–115 μm wide, hyaline, internally with medullary hyphae in layers or chambers, incrusted with yellowish hydrophobic granules which dissolve in K with an orange-yellow reaction. Epihymenium indistinct. Hymenium hyaline, 90–110 μm high. Subhymenium indistinct. Hypothecium hyaline, 25–50 μm high, K−. Apothecial base below hypothecium 100–125 μm high, hyaline, filled with medullary hyphae and yellowish granules in a continuous layer or chambers. Asci 80–90 × 15–22 μm, 4–6(–8)-spored. Ascospores simple, hyaline, 20–24(–25) × 10–13(–15) μm, wall uniformly thickened, halonate (1 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ orange-yellow, P−. TLC: unknown grey-brown spot at R f class 5 (UV+ orange).
Etymology
The epithet ‘lutea’ refers to the pale yellow colour of the apothecial disc in this species.
Remarks
This species is characterized by having a densely verrucose thallus, medulla of thallus and verrucae white to cream-coloured, and beige-coloured apothecial discs. It is similar to M. badimioides and M. glabromarginata (present paper) in having medulla of thallus and verrucae white to cream-coloured, granifera-type apothecial margins and an absence of atranorin (Kalb Reference Kalb2021). Both these species differ in having comparatively less verrucose thalli, smaller ascospores (14–20 × 7–10 μm in M. badimioides and 10–15 × 6–9 μm in M. glabromarginata vs 20–25 × 10–15 μm in this species), an orange-brown to dark brown hypothecium (hyaline in this species), and an apothecial disc that is orange-brown in M. glabromarginata and red-brown in M. badimioides (Kalb Reference Kalb2021).
Additional specimen examined
India: Uttarakhand: Bageshwar District, en route to Jatoli from Khati Village, 30°08ʹ30.33ʺN, 79°55ʹ21.57ʺE, 2440 m, 2023, Komal K. Ingle & R. S. Adhikari 23-047446 (LWG).
Malmidea palghatensis R. Adhikari & Nayaka sp. nov.
MycoBank No.: MB 852249
Similar to Malmidea duplomarginata but differs in having pink-coloured medulla of verrucae.
Type: Kerala, Palghat, Parambikulam Wildlife Sanctuary, 600 m, 14 November 2006, Biju Haridas 06-009856 (LWG—holotype).
(Fig. 6)
Figure 6. Habit of Malmidea palghatensis (holotype LWG- 06-009856). A, thallus. B, enlarged portion of thallus showing pink-coloured medulla of verrucae. C, apothecial section. Scales: A & B = 1 mm; C = 50 mm. In colour online.
Thallus corticolous, crustose, pale greyish, thick, continuous to cracked, verrucose; verrucae 0.2–0.28 mm diam. and 0.1 mm high, confluent to sparse, not prominent; isidia and soredia absent. Prothallus indistinct. Medulla of thallus white to creamish, medulla of verrucae pink-coloured.
Apothecia round to irregular, sessile, 1–3.5 mm diam.; disc plane to slightly convex, orange-brown to reddish-brown; margin granifera-type, concolorous with the thallus, thick, 90–110 μm thick, smooth, continuous, strongly flexuous. Thalline exciple 100–120 μm wide, densely filled with algal cells and pink-coloured granules, granules dissolving in K with a yellowish reaction. Proper exciple 25–30 μm, brown. Epihymenium pale to orange-brown, 12–15 μm high. Hymenium hyaline, 100–110 μm high. Subhymenium hyaline to pale, 10–15 μm high. Hypothecium 120–160 μm high, dark brown to black, K−. Apothecial base below hypothecium 150–160 μm high, hyaline. Asci 80–90 × 18–22 μm, (4–)6–8-spored. Ascospores simple, hyaline, ellipsoid, 17–22(–24) × 10–12(–13) μm, wall uniformly thickened, halonate (2–3 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ orange-yellow, P−. TLC: atranorin together with an unknown purple-violet spot.
Etymology
The species epithet ‘palghatensis’ is based on the name of the locality from where the holotype is collected.
Remarks
This species is characterized by having a thalline excipulum and pink-coloured medulla of verrucae. Malmidea duplomarginata also has a thalline excipulum but differs in having a white to cream-coloured medulla of verrucae and smaller apothecia up to 1.5 mm diam. (Kalb et al. Reference Kalb, Buaruang, Papong and Boonpragob2009). Malmidea kalbii and M. incrassatispora described above also have a thalline excipulum. Malmidea kalbii differs in having smaller ascospores, 9–15 × 6–9 μm, and a whitish medulla of verrucae. Malmidea incrassatispora differs in having ascospores with distinct end wall thickenings and a yellowish medulla of verrucae.
Additional specimen examined
India: Odisha: Kandhamal District, Raikia, near Mandasaru valley, 19°58ʹ27ʺN, 84°14ʹ27ʺE, 726 m, 2019, R. R. Paul 19-039538/C (LWG).
Malmidea rubra R. Adhikari & Nayaka sp. nov.
MycoBank No.: MB 852250
Similar to Malmidea granifera (Ach.) Kalb et al., from which it differs in having an orange-red pigmented (vs yellowish to peach-coloured) medulla of verrucae and light-brown (vs dark brown to black) apothecial discs.
Type: India, Arunachal Pradesh, West Siang District, Bahadur Hill Reserve Forest, 11 April 2006, Urvashi Dubey 06-006408 (LWG—holotype).
(Fig. 7)
Figure 7. Habit of Malmidea rubra (holotype LWG- 06-006408). A, thallus. B, enlarged portion of thallus showing orange-red coloured medulla of verrucae. Scales: A & B = 1 mm. In colour online.
Thallus crustose, corticolous, greenish grey, continuous, smooth to irregularly verrucose; verrucae sparse, not prominent; isidia and soredia absent. Prothallus greyish brown to black. Medulla of thallus creamish to faintly yellow, medulla of verrucae orange-red to dark red.
Apothecia round, sessile, 0.5–1 mm diam.; disc light to dark orange-brown, plane to slightly convex; margin granifera-type, moderately thick, 55–70 μm thick, continuous, smooth to papillate, apically whitish. Thalline exciple absent. Proper exciple 70–120 μm wide, externally pale brown, internally dark brown to black, with medullary hyphae in chambers, incrusted with yellowish hydrophobic granules, K+ intense yellow. Epihymenium indistinct or slightly pale. Hymenium hyaline, 55–70 μm high. Subhymenium hyaline to pale, 12–17 μm high. Hypothecium 120–160 μm high, dark brown to black, K−. Apothecial base indistinct from hypothecium. Asci 50–75 × 15–20 μm, 4–8-spored. Ascospores simple, hyaline, ellipsoid, 14–18(–20) × 6–9(–10) μm, wall evenly thickened, halonate (1–1.5 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ red, P−. TLC: atranorin (minor).
Etymology
The epithet ‘rubra’ is based on its orange-red coloured medulla of verrucae.
Remarks
This species is characterized by having irregularly distributed, fine verrucae, medulla of thallus creamish to faintly yellow, medulla of verrucae orange-red to dark red and whitish apothecial margins. The other species of Malmidea with a pigmented medulla, granifera-type exciple and similar ascospore size are M. eeuuae Kalb, M. granifera and M. subaurigera (Vain.) Kalb et al. (Breuss & Lücking Reference Breuss and Lücking2015). Malmidea granifera differs in having yellowish to peach-coloured medulla of verrucae, dark brown to black apothecial discs, 8-spored asci and a different chemistry (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011; Breuss & Lücking Reference Breuss and Lücking2015). Malmidea subaurigera and M. eeuuae both differ in having a thallus with well-developed regular warts and an apothecial margin that becomes granular and excluded towards maturity (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011). Furthermore, the medulla of verrucae is creamish to sulphur yellowish in M. eeuuae and in M. subaurigera the medulla of both the thallus and verrucae is orange-red pigmented (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011). In having whitish apothecial margins, it is also similar to M. albomarginata Kalb & Hernández but in this species the medulla of verrucae is white to faintly yellow-pigmented, the warts become coralloid granular with age, and it has smaller ascospores (10–14 × 6–9 μm) (Kalb Reference Kalb2021).
Additional specimens examined
India: Arunachal Pradesh: West Siang District, Bahadur Hill Reserve Forest, 2006, Urvashi Dubey 06-006408, 06-006412, 06-006417, 06-006463 & 06-006488 (LWG).
Malmidea subindica R. Adhikari & Nayaka sp. nov.
MycoBank No.: MB 852251
Similar to Malmidea indica (D. D. Awasthi & M. R. Agarwal) Hafellner & T. Sprib. and Malmidea sorsogona (Vain.) Kalb et al. but differing in its slightly (vs distinctly) verrucose thallus and light to dark orange-brown (vs dark brown to black) apothecial discs.
Type: India, Sikkim, East Sikkim, Rumtek, 1500 m, 4 September 2000, Chatterjee & Divakar 20-77064/A (LWG—holotype).
(Fig. 8)
Figure 8. Malmidea subindica (holotype LWG-20-77064/A). A, thallus. B, 4-spored ascus. C, ascus lacking a tubular structure in the tholus (in Lugol's iodine solution). Scales: A = 1 mm; B & C = 20 μm. In colour online.
Thallus crustose, corticolous, continuous, dark greenish grey, verrucose; verrucae confluent, 0.15–0.2 mm diam., height < 0.1 mm; isidia and soredia absent. Prothallus brownish black. Medulla of thallus white, medulla of verrucae creamish to faintly yellow.
Apothecia sessile, round, 0.4–1 mm diam.; disc plane to slightly convex, light to dark orange-brown; margin granifera-type, continuous, smooth to slightly granular, 50–60 μm thick, whitish to cream-coloured. Thalline exciple absent. Proper exciple 50–95 μm wide, externally hyaline, internally brownish with medullary hyphae and hydrophobic granules in chambers, granules dissolving in K with a lemon yellow reaction. Epihymenium indistinct. Hymenium hyaline, 100–110 μm high. Subhymenium 30–40 μm thick, hyaline. Hypothecium 65–80 μm high, orange-brown to dark brown, K−. Apothecial base below hypothecium 100–120 μm high, hyaline with medullary hyphae and granules in chambers. Asci 75–100 × 18–25 μm, 2–4-spored. Ascospores simple, hyaline, ellipsoid, 28–35(–40) × 17–21(–24) μm, wall evenly thickened, 1.5–2 μm thick, halonate (1–2.5 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ deep yellow, P−. TLC: two unknown grey-brown spots at R f class 5 and 6 in solvent system A.
Etymology
The epithet ‘subindica’ refers to the close resemblance of the species to Malmidea indica.
Remarks
This species is characterized by having light to dark orange-brown apothecial discs, 2–4-spored asci, and broadly ellipsoid ascospores mostly exceeding 30 μm in length. It is similar to M. indica and M. sorsogona in having ascospores with evenly thick walls and lengths exceeding 25 μm but both these species have dark brown to black apothecial discs and a sparsely verrucose thallus (Vainio Reference Vainio1921; Awasthi & Agarwal Reference Awasthi and Agarwal1968; Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011). Malmidea indica has 2–3-spored asci and longer and narrower ascospores, 31–39 × 12–18 μm (Awasthi & Agarwal Reference Awasthi and Agarwal1968). Furthermore, the chemistry of the holotype of M. indica showed the presence of atranorin. Malmidea sorsogona has smaller and narrower ascospores, 22–34 × 9–16 μm, with the medulla of verrucae yellowish turning orange-red in K (Vainio Reference Vainio1921). It is also close to M. upretii (described below) but that species has dark brown to blackish apothecial discs, a whitish grey thallus with prominent and confluent verrucae and an orange-red medulla.
Additional specimen examined
India: Sikkim: East Sikkim, Rumtek, 1500 m, 2000, Chatterjee & Divakar 20-77064/B (LWG).
Malmidea upretii R. Adhikari, Ingle & Nayaka sp. nov.
MycoBank No.: MB 852252
Similar to Malmidea indica and M. sorsogona but differs in having prominent and confluent verrucae with medulla orange-red coloured.
Type: India, Goa, Mollem, Bhagwan Mahavir Wildlife Sanctuary, May 1983, A. Singh & D. K. Upreti L-11490/A (LWG—holotype).
(Fig. 9)
Figure 9. Habit of Malmidea upretii (holotype LWG- L-11490/A). A, thallus. B, enlarged portion of thallus showing orange-red pigmented medulla of verrucae. Scales: A & B = 1 mm. In colour online.
Thallus crustose, corticolous, whitish grey, thick, continuous, verrucose; verrucae prominent and confluent, 0.12–0.2 mm diam. and 0.1 mm high; isidia and soredia absent. Prothallus indistinct. Medulla of thallus whitish to cream-coloured, medulla of verrucae orange-red.
Apothecia round, sessile, up to 1.3 mm diam.; disc orange-brown to black, plane to slightly convex; margin granifera-type, thick, 90–110 μm thick, not prominently raised, grey-brown turning black, continuous, smooth to slightly papillate, persistent. Thalline exciple absent. Proper exciple 100–140 μm wide, externally hyaline to pale, internally brownish, filled with medullary hyphae and creamish to pale granules which dissolve in K with a lemon yellow to orange-yellow reaction. Epihymenium indistinct. Hymenium hyaline, 110–120 μm high. Subhymenium 15–20 μm high, hyaline to pale. Hypothecium dark brown, 75–95 μm high, K−. Apothecial base below hypothecium hyaline, 100–130 μm high, filled with medullary hyphae and pale granules. Asci 65–90 × 18–22 μm, 2–3-spored. Ascospores simple, hyaline, broadly ellipsoid, 24–30(–34) × 13–17(–19) μm, wall uniformly thick, halonate (1.5–2 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ deep red, P−. TLC: atranorin only.
Etymology
This species is named in honour of Dr D. K. Upreti, an eminent Indian lichenologist.
Remarks
This species is characterized by a whitish grey thallus with prominent and confluent verrucae, with medulla of verrucae orange-red and ascospores exceeding 25 μm in length. This species is similar to M. indica, M. sorsogona and M. subindica, described in the present paper, by having ascospores with uniformly thick walls and lengths exceeding 25 μm. Malmidea indica differs in having a granulose to granular verrucose thallus with sparse verrucae, with medulla of verrucae faintly yellowish and larger ascospores, 31–39 × 12–18 μm (Awasthi & Agarwal Reference Awasthi and Agarwal1968). Malmidea sorsogona has a sparsely verrucose thallus, 4-spored asci and medulla of verrucae yellowish (Vainio Reference Vainio1921; Breuss & Lücking Reference Breuss and Lücking2015). Malmidea subindica differs in having orange-brown coloured apothecial discs with thin margins and medulla of verrucae creamish to faintly yellow; it also lacks atranorin.
Additional specimens examined
India: Goa: Mollem, Bhagwan Mahavir Wildlife Sanctuary, 1983, A. Singh & D. K. Upreti L-11490/B (LWG). West Bengal: Darjeeling District, Kalimpong division, on way to Munsog from Kalimpong, 1500 m, D. D. Awasthi & M. R. Agarwal 67.328/G (LWG).
Malmidea verrucosa R. Adhikari, R. Ngangom & Nayaka sp. nov.
MycoBank No.: MB 852253
Similar to Malmidea eeuuae and M. subaurigera but differs in having confluent and prominent verrucae, darker and entire apothecial margins and by containing atranorin.
Type: India, Uttarakhand, Bageshwar District, en route to Jatoli from Khati Village, 30°08ʹ30.33ʺN, 79°55ʹ21.57ʺE, 2440 m, 3 December 2021, S. Nayaka, R. S. Adhikari & R. Ngangom 21-045440 (LWG—holotype).
(Fig. 10)
Figure 10. Habit of Malmidea verrucosa (holotype LWG- 21-045440). Scale = 1 mm. In colour online.
Thallus crustose, corticolous, whitish to whitish grey, verrucose; verrucae prominent and confluent, round to coralloid, 0.2–0.3 mm diam., 0.1–0.2 mm high; isidia and soredia absent. Prothallus indistinct. Medulla of thallus whitish to cream-coloured, medulla of verrucae pale to yellowish.
Apothecia round, sessile, 0.5–1.4 mm diam.; disc orange-brown to chocolate brown, plane to slightly concave; margin granifera-type, greyish black, 80–100 μm thick, continuous, smooth, becoming flexuous. Exciple lacking algal cells, externally hyaline with medullary hyphae and creamish to yellowish hydrophobic granules partly dissolving in K with a lemon yellow to greenish yellow reaction. Epihymenium slightly pale, 10–15 μm high. Hymenium 80–95 μm high, hyaline. Hypothecium 80–135 μm high, dark brown to black, K−. Apothecial base below hypothecium 100–130 μm high, hyaline, with medullary hyphae and yellowish granules in chambers. Asci 70–95 × 15–22 μm, (2–)4–6-spored. Ascospores simple, hyaline, 17–22(–25) × 10–12(–13) μm, wall evenly thickened, halonate (1–2 μm wide).
Pycnidia not observed.
Chemistry
Medulla of thallus K+ yellow, P−; medulla of verrucae K+ orange-yellow, P−. TLC: atranorin.
Etymology
The species epithet ‘verrucosa’ refers to the presence of prominent and confluent verrucae.
Remarks
This species is characterized by having a whitish grey, densely verrucose thallus and orange-brown to chocolate brown apothecial discs. The medulla of verrucae is pigmented, with granifera-type apothecial margins, a dark brown hypothecium, and in ascospore size, this species is close to M. eeuuae, M. fenicis and M. subaurigera. Malmidea eeuuae and M. subaurigera differ in having comparatively sparse verrucae, a greenish grey thallus (whitish grey in this species), whitish to cream-coloured apothecial margins and the medulla of verrucae differing in colour (cream to bright sulphur yellow in M. eeuuae and orange-yellow in M. subaurigera), both also lack atranorin (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011). Furthermore, in M. eeuuae and M. subaurigera the apothecial margins become warty with age and finally disappear (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011), while remaining entire in the new species. Malmidea fenicis differs in having distinctly black apothecial discs with thin margins (Vainio Reference Vainio1921; Breuss & Lücking Reference Breuss and Lücking2015). In having confluent and coralloid verrucae, the new species is also similar to M. coralliformis Kalb, but this differs in the whitish medulla in the thallus and verrucae, and absence of atranorin (Kalb et al. Reference Kalb, Rivas Plata, Lücking and Lumbsch2011).
Additional specimens examined
India: Uttarakhand: Bageshwar District, en route to Jatoli from Khati Village, 30°08ʹ30.33ʺN, 79°55ʹ21.57ʺE, 2440 m, 2021, S. Nayaka, R. S. Adhikari & R. Ngangom 21-045520 (LWG); Uttarkashi District, Govind Wildlife Sanctuary, way to Kedarkantha, 31°03.833ʺN, 78°11.26ʺE, 2324 m, D. K. Upreti, S. Nayaka & R. Bajpai 11-016086(LWG).
New Records
Malmidea fenicis (Vain.) Kalb, Rivas Plata & Lumbsch
Characterized by a crustose, corticolous, verrucose thallus, with a white, K+ yellow medulla and the medulla of verrucae yellow, K+ orange-red. The apothecial disc is black, and the margin granifera-type, greyish brown to black (Fig. 11). Asci are 6–8-spored, ascospores simple, hyaline, 18–25 × 9–13 μm, and the wall evenly thickened. No chemical detected by TLC. This species is previously known from its type locality in the Philippines only (Paguirigan et al. Reference Paguirigan, dela Cruz, Santiago, Gerlach and Aptroot2020).
Figure 11. New records. A, Malmidea fenicis (LWG-89436). B, M. leptoloma (LWG-67685). C, M. piae (LWG-18-036012). D, M. piperina (LWG-13-021839). E, M. reunionis (LWG-18-029254). F, M. sulphureosorediata (LWG-79716). G, M. vinosa (LWG-99-75945). Scales: A–G = 1 mm. In colour online.
Specimen examined
India: Andaman Islands: Middle Andaman, Long Island, 1961, A. Singh 89436 (LWG). Karnataka: Uttara Kannada, Siddapur, Mattigar, 2019, D. K. Upreti, G. K. Mishra & S. Dudani 19-036438 (LWG).
Malmidea leptoloma (Müll. Arg.) Kalb & Lücking
Characterized by a thallus that is crustose, corticolous, rugulose, ±smooth with a white, K− medulla. The apothecial disc is beige to ochre-brown with a piperis-type margin that is concolorous to disc, apically turning black (Fig. 11). Asci are 8-spored, and ascospores 9–15 × 5–7 μm. No chemical detected by TLC. Previously known from Australia, Bolivia, Brazil and the Solomon Islands (Bailey Reference Bailey1896; Lücking et al. Reference Lücking, Seavey, Common, Beeching, Breuss, Buck, Crane, Hodges, Hodkinson and Lay2011; Flakus et al. Reference Flakus, Sipman, Bach, Flakus, Knudsen, Ahti, Schiefelbein, Palice, Jabłońska and Oset2013; Kalb Reference Kalb2021; Kantvilas et al. Reference Kantvilas, Wedin and Svensson2021).
Specimen examined
India: Andaman Islands: Middle Andaman, Long Island, 1961, A. Singh 67685 (LWG).
Malmidea piae (Kalb) Kalb
Thallus is crustose, corticolous, verrucose, with verrucae developing into polysidiangia-like clumps, bursting apically and exposing the medulla; medulla of thallus and verrucae lemon yellow, K+ orange. Apothecia are sessile, the disc light to dark brown, and the granifera-type margin white to cream-coloured (Fig. 11). Asci are 4–8-spored, ascospores simple, hyaline, 15–22 × 9–12 μm, and the wall evenly thickened. Atranorin detected by TLC. Previously reported from Australia and Thailand (Kalb et al. Reference Kalb, Buaruang, Papong and Boonpragob2009).
Specimens examined
India: Nagaland: Tuensang District, Konya Village, 26°15ʹ53.00ʺN, 94°53ʹ11.00ʺE, 1754 m, 2018, R. Ngangom 18-036012 (LWG). Uttarakhand: Dehradun District, Chandrabani Wildlife Institute of India campus, 2013, Adhikari 13-025347 (LWG).
Malmidea piperina (Zahlbr.) Aptroot & Breuss
Thallus is crustose, corticolous, verrucose, and medulla of verrucae yellow, K+ orange. Apothecia are sessile, 0.6 mm diam., the disc black, and granifera-type margin papillate and concolorous with the disc (Fig. 11). Asci are 8-spored, ascospores simple, hyaline, 10–15 × 5–7 μm, and wall evenly thickened. No chemical detected by TLC. Previously known from Brazil, Sri Lanka and Taiwan (Wang-Yang & Lai Reference Wang-Yang and Lai1973; Weerakoon et al. Reference Weerakoon, Wolseley, Arachchige, Cáceres, Jayalal and Aptroot2016; Oliveira Junior et al. Reference Oliveira Junior, Aptroot and Cáceres2021).
Specimens examined
India: Andaman Islands: Middle Andaman, Bajalungta, 1961, A. Singh 52945 (LWG); South Andaman, T.L.D. range, 90 m, 1961, A. Singh & party 88221 (LWG). Assam: Nagaon District, Chapanala Village, 60 m, 2013, Amit Kumar Dey 13-021839 (LWG). Mizoram: MZU campus, 2018, Nurpen Meitei Thangjam 33 (LWG).
Malmidea reunionis Kalb
Thallus is crustose, corticolous, verrucose, and medulla of verrucae cream to yellowish, K+ orange. The apothecial disc is brown and the margin granifera-type and cream-coloured (Fig. 11). Asci are 6–8-spored, ascospores simple, hyaline, 22–30 × 12–15 μm, and walls thickened at ends. Unknown substances detected by TLC. Previously known from its type locality of Reunion Island only (Kalb et al. Reference Kalb, Buaruang, Mongkolsuk and Boonpragob2012).
Specimen examined
India: Karnataka: Uttara Kannada, Dandeli, Singargao cross, 15°19ʹ55.6ʺN, 74°32ʹ38.9ʺE, 640 m, 2018, S. Nayaka, S. Joseph & S. Dudani 18-029254 (LWG).
Malmidea sulphureosorediata M. Cáceres, D. A. Mota & Aptroot
Characterized by a crustose, corticolous, thin, densely sorediate thallus, lacking verrucae. Medulla and soredia bright yellow, K+ orange-red (Fig. 11). Apothecia are absent. Unknown substances detected by TLC. Previously known from Brazil only (Cáceres et al. Reference Cáceres, dos Santos, Góes, Mota and Aptroot2013).
Specimen examined
India: Andaman Islands: South Andaman group, Nilambur, Baratang Island, 30 m, 1961, A. Singh 79716 (LWG).
Malmidea vinosa (Eschw.) Kalb, Rivas Plata & Lumbsch
Thallus is crustose, corticolous, slightly rough, wrinkled. Medulla white, K−. Apothecial disc is brownish, the margin piperis-type, thick and dark greyish (Fig. 11). Asci are 6–8-spored, ascospores simple, hyaline, 10–20 × 6–8 μm, and wall evenly thickened. No chemical detected by TLC. Previously reported from Bermuda, Bolivia, Brazil, Guyana and Florida (Flakus et al. Reference Flakus, Sipman, Bach, Flakus, Knudsen, Ahti, Schiefelbein, Palice, Jabłońska and Oset2013; Berger & LaGreca Reference Berger and LaGreca2014; Aptroot & Souza Reference Aptroot and Souza2021).
Specimen examined
India: Tamil Nadu: Kambam District, Meghamalai Wildlife Sanctuary, Vannatiiparai, 400 m, 1999, S. Nayaka 99-75945 (LWG).
Corresponding positions of Malmidea species (discovered between 2015–2023) in the world key by Breuss & Lücking (Reference Breuss and Lücking2015)
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Couplet 6 Isidia verruciform to shortly cylindrical; apothecial margin granifera-type; ascospores 11–12.5 × 4.5–5.5 μm; atranorin present ……… M. densisidiata Aptroot & Oliveira-Junior (Aptroot et al. Reference Aptroot, Souza, dos Santos, Junior, Barbosa and Cáceres2022)
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Couplet 6 Isidia verruciform to shortly cylindrical; apothecial margin granifera-type; 12–15 × 7–8 μm; atranorin absent ……… M. isidiopiperina Lücking et al. (Lücking et al. Reference Lücking, Álvaro-Alba, Moncada, Marín-Canchala, Tunjano and Cárdenas-López2023)
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Couplet 7 Isidia granular to coralloid; medulla orange-red; atranorin and norsolorinic acid present; ascospores 17–23 × 7–10 μm ……… M. isidiifera Kalb (Kalb Reference Kalb2021)
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Couplet 7 Isidia granular to coralloid; medulla white; ascospores 14–22 × 8–10 μm; lichen substances absent ……… M. hernandeziana Kalb (Kalb Reference Kalb2021)
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Couplet 7 Isidia distinctly granular; medulla orange-red; ascospores 15–20 × 9–12 μm; norsolorinic acid present ……… M. rhodopisoides Kalb (Kalb Reference Kalb2021)
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Couplet 8 Medulla pale yellow; apothecial disc brown, margin cream-coloured to brown, granifera-type; ascospores 12–16 × 7.5–9 μm ……… M. attenboroughii Kukwa et al. (Guzow-Krzemińska et al. Reference Guzow-Krzemińska, Flakus, Kosecka, Jabłońska, Rodriguez-Flakus and Kukwa2019)
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Couplet 10 Medulla orange-yellow, K+ purple; apothecial disc beige to chocolate brown, margin cream-coloured to greyish, thin; ascospores 11–17 × 7–10 μm ……… M. volcaniana Kalb & Hernández (Kalb Reference Kalb2021)
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Couplet 13 Ascospores 28–40 × 17–24 μm, 2–4 per ascus; thallus verrucae confluent, not prominent; atranorin absent ……… M. subindica R. Adhikari & Nayaka (this paper)
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Couplet 16 Ascospores 24–34 × 13–19 μm, 2–3 per ascus; medulla of verrucae orange-red, K+ deep red ……… M. upretii R. Adhikari et al. (this paper)
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Couplet 17 Apothecia with a continuous or granular thalline excipulum surrounding the proper excipulum; disc dark brown to black; ascospores 9–15 × 6–9 μm ……… M. kalbii R. Adhikari & Nayaka (this paper)
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Couplet 17 Apothecia with a continuous or granular thalline excipulum surrounding the proper excipulum; disc reddish brown; ascospores 20–27 × 10–14 μm with end wall thickenings ……… M. incrassatispora R. Adhikari et al. (this paper)
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Couplet 17 Apothecia with a continuous thalline excipulum surrounding the proper excipulum; disc light to dark orange-brown; medulla of verrucae pink-coloured; ascospores 17–24 × 10–13 μm ……… M. palghatensis R. Adhikari & Nayaka (this paper)
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Couplet 20 Apothecial disc beige, margin thick, pale; hypothecium hyaline; ascospores 20–25 × 10–15 μm ……… M. lutea R. Adhikari et al. (this paper)
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Couplet 23 Thallus verrucae conspicuous (0.1–0.25 mm diam.), medulla of thallus and verrucae K+ lemon yellow, P+ orange; ascospores 13–17 × 7–9 μm ……… M. hechicerae Kalb (Kalb Reference Kalb2021)
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Couplet 24 Ascospores mainly 4–8 per ascus, 10–15 × 6–9 μm; medulla of thallus and verrucae K+ bright yellow; apothecial margin distinctly white, entire; lichen substances absent ……… M. glabromarginata R. Adhikari et al. (this paper)
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Couplet 25 Medulla of thallus and verrucae white to faintly yellow, K+ orange-red; ascospores 10–14 × 7–8 μm; atranorin absent ……… M. allobakeri Kalb & M. Cáceres (Kalb Reference Kalb2021)
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Couplet 30 Excipulum with a continuous medullary layer; verrucae rare becoming coralloid granular with age; medulla of thallus and verrucae white to faintly yellow ……… M. albomarginata Kalb & Hernández (Kalb Reference Kalb2021)
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Couplet 32 Apothecial disc beige to light brownish, margin thick, bulging, white to cream-coloured medulla of excipulum K+ lemon yellow; hypothecium dark brown; medulla of thallus and verrucae orange-yellow, K+ orange-red ……… M. atlanticoides Kalb & M. Cáceres (Kalb Reference Kalb2021)
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Couplet 32 Apothecial disc light to dark brown; margin thin, whitish grey to dark brownish grey; medulla of excipulum K+ greenish yellow; hypothecium dark brown; medulla of thallus verrucae peach-coloured to pink, K+ orange-red ……… M. allopapillosa Kalb (Kalb Reference Kalb2021)
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Couplet 35 Apothecial disc orange-brown to chocolate brown; ascospores 2–6 per ascus, 17–25 × 10–13 μm, hypothecium 80–135 μm high; medulla of verrucae pale to yellowish ……… M. verrucosa R. Adhikari et al. (this paper)
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Couplet 35 Apothecial disc light to dark orange-brown; ascospores 4–8 per ascus, 14–20 × 6–10 μm, hypothecium 120–160 μm high; medulla of verrucae orange-red to dark red ……… M. rubra R. Adhikari & Nayaka (this paper)
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Couplet 38 Apothecia 0.3–0.6 mm diam., disc light beige, brown-grey to brown, margins whitish grey to dark brown-black; thallus smooth, cortex abraded; ascospores 9–12 × 4–6 μm ……… M. leucopiperis Kalb (Kalb Reference Kalb2021)
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Couplet 38 Apothecia 0.5–1 mm diam., disc dark brown, margins whitish; thallus granulose-verrucose; ascospores 8–15 × 5–7.5 μm ……… M. nagalandica (G. P. Sinha & Kr. P. Singh) G. P. Sinha et al. (Singh & Pinokiyo Reference Singh and Pinokiyo2014)
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Couplet 41 Apothecial margin brownish grey, disc grey-brown to brown; thallus ±smooth ……… M. fulva (Malme) Kalb & van den Boom (van den Boom et al. Reference van den Boom, Kalb and Elix2014)
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Couplet 44 Medulla of verrucae pale yellow, K+ orange; medulla of thallus white; apothecial disc brown, margin pale; ascospores 9–15 × 6–9 μm ……… M. globosa R. Adhikari & Nayaka (this paper)
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Couplet 46 Apothecial margins black; ascospores 7–9 × 3.5 μm ……… M. demutans (Nyl.) Lücking (Lücking et al. Reference Lücking, Moncada and Soto-Medina2021)
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Couplet 49 Thallus grey to grey-brown; apothecial disc dark brown, margin slightly prominent, pale bluish black; ascospores 9–16 × 5.5–6.5 μm ……… M. floridensis (Nyl.) M. Cáceres et al. (Cáceres et al. Reference Cáceres, Aptroot, Mendonça, Santos and Lücking2017)
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Couplet 49 Thallus dull, grey, plicate folded; apothecial disc brown-grey, margin prominent, pale ochraceous; ascospores 7.5–8.5 × 3.0–3.5 μm ……… M. plicata Weerakoon & Aptroot (Weerakoon et al. Reference Weerakoon, Wolseley, Arachchige, Cáceres, Jayalal and Aptroot2016)
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Couplet 49 Thallus pale greenish at the margin, almost black in most parts; apothecial disc pale brown, margin prominent, grey; ascospores 10–11.5 × 2.5–3.5 μm ……… M. nigra Aptroot & Oliveira Junior (Aptroot et al. Reference Aptroot, Souza, dos Santos, Junior, Barbosa and Cáceres2022)
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Couplet 49 Thallus grey, greenish grey to olive; apothecial disc beige to greyish brown, margin whitish grey to brownish grey, moderately thick; ascospores 9–12 × 5–7 μm ……… M. subcinerea Kalb (Kalb Reference Kalb2021)
Acknowledgements
The authors are grateful to the Director, CSIR-National Botanical Research Institute, Lucknow for providing laboratory facilities under the project OLP0114, and to Dr D. K. Upreti for his valuable suggestions during the identification of lichen samples and preparation of this manuscript. RA and RN thank the University Grant Commission, New Delhi for providing financial assistance in the form of JRF and SRF respectively. SJ is grateful to Dr Syam Viswanath, Director, KSCSTE-Kerala Forest Research Institute, Peechi, for providing laboratory facilities. The authors also thank the previous collectors (Amit Kumar Dey, A. R. Logesh, Biju Haridas, Chinlapianga, D. K. Upreti, G. K. Mishra, Nurpen Meitei Thangiam, P. K. Divakar, R. Bajpai, R. R. Paul, S. Dudani, S. Chatterjee, S. Pradhan, S. R. Singh and Urvashi Dubey) and their funding agencies, whose samples are deposited in the herbarium LWG including CSIR, New Delhi (project BSC 106) and SERB, New Delhi (Young Scientist Project). The authors are grateful to Prof. Dr Klaus Kalb (Lichenologisches Institut Neumarkt, Germany) for providing literature and valuable suggestions. This manuscript is allotted number CSIR-NBRI_MS/2023/12/04 by the institute's publication ethics committee.
Author ORCIDs
Rakesh Adhikari, 0000-0003-0403-864X; Roshinikumar Ngangom, 0000-0002-1534-0951; Komal K. Ingle, 0000-0002-0659-6031; Siljo Joseph, 0000-0002-8027-7936; Sanjeeva Nayaka, 0000-0001-6541-2362.
Competing Interests
The authors declare none.
