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Alternative Methods of extracting and Amplifying Dna from lichens

Published online by Cambridge University Press:  28 March 2007

Maria P. Martín  and
Katarina Winka
Maria P. Martín
Affiliation:
Departamento de Biologia Vegetal (Botanica) Facultad de Biologĺa, Avda. Diagonal 645, 08028–Barcelona, Spain.
Katarina Winka
Affiliation:
Department of Ecology and Environmental Science, Umeåa University, SE-90187 Umeaå, Sweden.
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Abstract

We have investigated whether DNA extraction protocols designed specifically for fungi and/or lichens perform better on lichens than do corresponding protocols designed for plants and insects. Two different PCR-amplification protocols were used to evaluate the quality of the DNA extracted with each method. The DNA extractions with highest quality were obtained with the protocols designed for insects and plants, and the most successful amplifications were obtained with Ready-To-Go PCR Beads. This indicates that fungal or lichen specific protocols might not be necessary for successful extraction of high quality DNA from lichens.

Type
Research Article
Information
The Lichenologist , Volume 32 , Issue 2 , March 2000 , pp. 189 - 196
Copyright
Copyright © British Lichen Society 2000

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