Alternative Methods of extracting and Amplifying Dna from lichens

Maria P. Martín; Katarina Winka

doi:10.1006/lich.1999.0254

Abstract

We have investigated whether DNA extraction protocols designed specifically for fungi and/or lichens perform better on lichens than do corresponding protocols designed for plants and insects. Two different PCR-amplification protocols were used to evaluate the quality of the DNA extracted with each method. The DNA extractions with highest quality were obtained with the protocols designed for insects and plants, and the most successful amplifications were obtained with Ready-To-Go PCR Beads. This indicates that fungal or lichen specific protocols might not be necessary for successful extraction of high quality DNA from lichens.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Esslinger, Theodore L. 2000. Recent Literature on Lichens—179*. The Bryologist, Vol. 103, Issue. 4, p. 816.

Martín, M. P. Winka, K. Llimona, X. and Lumbsch, H. T. 2000. Evaluation of Morphological Variation in the Lichen Diploschistes ocellatus (Ascomycota, Ostropales): Evidence from Nuclear rDNA ITS Sequence Data. Plant Biology, Vol. 2, Issue. 5, p. 571.

Stenroos, Soili Hyvönen, Jaakko Myllys, Leena Thell, Arne and Ahti, Teuvo 2002. Phylogeny of the Genus Cladonia s.lat. (Cladoniaceae, Ascomycetes) Inferred from Molecular, Morphological, and Chemical Data. Cladistics, Vol. 18, Issue. 3, p. 237.

Scherrer, Sandra and Honegger, Rosmarie 2003. Inter‐ and intraspecific variation of homologous hydrophobin (H1) gene sequences amongXanthoriaspp. (lichen‐forming ascomycetes). New Phytologist, Vol. 158, Issue. 2, p. 375.

Martı́n, Marı́a P Coucheron, Dag H and Johansen, Steinar 2003. Structural features and evolutionary considerations of group IB introns in SSU rDNA of the lichen fungus Teloschistes. Fungal Genetics and Biology, Vol. 40, Issue. 3, p. 252.

Gaya, Ester Lutzoni, François Zoller, Stefan and Navarro‐Rosinés, Pere 2003. Phylogenetic study of Fulgensia and allied Caloplaca and Xanthoria species (Teloschistaceae, lichen‐forming ascomycota). American Journal of Botany, Vol. 90, Issue. 7, p. 1095.

DePriest, Paula T. 2004. Early Molecular Investigations of Lichen-Forming Symbionts: 1986–2001. Annual Review of Microbiology, Vol. 58, Issue. 1, p. 273.

Thorn, R. Greg Moncalvo, Jean-Marc Redhead, Scott A. Lodge, D. Jean and Martín, María P. 2005. A new poroid species ofResupinatusfrom Puerto Rico, with a reassessment of the cyphelloid genusStigmatolemma. Mycologia, Vol. 97, Issue. 5, p. 1140.

Phosri, Cherdchai Martín, María P. Sihanonth, Prakitsin Whalley, Anthony J.S. and Watling, Roy 2007. Molecular study of the genus Astraeus. Mycological Research, Vol. 111, Issue. 3, p. 275.

Ezzat Ahma, M. Noormohamm, Gh. Ghodsi, M. and Kafi, M. 2009. Effects of Water Deficit and Spraying of Dessicant on Yield, Yield Components and Water Use Efficiency of Wheat Genotypes. Pakistan Journal of Biological Sciences, Vol. 12, Issue. 21, p. 1399.

KRZEWICKA, Beata GARCÍA, Miguel A. JOHANSEN, Steinar D. SANCHO, Leopoldo G. and MARTÍN, María P. 2009. Morphological and nuclear ribosomal DNA data support distinguishing two new species ofUmbilicaria(Umbilicariaceae, Ascomycota) from Europe. The Lichenologist, Vol. 41, Issue. 6, p. 631.

Telleria, M. Teresa Dueñas, Margarita Melo, Ireneia Hallenberg, Nils and Martín, María P. 2010. A re-evaluation of Hypochnicium (Polyporales) based on morphological and molecular characters. Mycologia, Vol. 102, Issue. 6, p. 1426.

Telleria, M. Teresa Dueñas, Margarita Melo, Ireneia and Martín, María P. 2010. Morphological and molecular studies of Hyphodermella in the Western Mediterranean area. Mycological Progress, Vol. 9, Issue. 4, p. 585.

Suz, Laura M. Martín, María P. Fischer, Christine R. Bonet, José A. and Colinas, Carlos 2010. Can NPK fertilizers enhance seedling growth and mycorrhizal status of Tuber melanosporum-inoculated Quercus ilex seedlings?. Mycorrhiza, Vol. 20, Issue. 5, p. 349.

PINO-BODAS, RAQUEL MARTÍN, MARÍA P. and BURGAZ, ANA R. 2010. Insight into theCladonia convoluta-C. foliacea(Cladoniaceae, Ascomycota) complex and related species, revealed through morphological, biochemical and phylogenetic analyses. Systematics and Biodiversity, Vol. 8, Issue. 4, p. 575.

Kovács, Gábor M. Balázs, Tímea K. Calonge, Francisco D. and Martín, María P. 2011. The diversity of Terfezia desert truffles: new species and a highly variable species complex with intrasporocarpic nrDNA ITS heterogeneity. Mycologia, Vol. 103, Issue. 4, p. 841.

Kelly, Laura J. Hollingsworth, Peter M. Coppins, Brian J. Ellis, Christopher J. Harrold, Paul Tosh, James and Yahr, Rebecca 2011. DNA barcoding of lichenized fungi demonstrates high identification success in a floristic context. New Phytologist, Vol. 191, Issue. 1, p. 288.

Olariaga, Ibai Grebenc, Tine Salcedo, Isabel and Martín, María P. 2012. Two new species of Hydnum with ovoid basidiospores: H. ovoideisporum and H. vesterholtii. Mycologia, Vol. 104, Issue. 6, p. 1443.

Phosri, C. Martín, M.P. Suwannasai, N. Sihanonth, P. and Watling, R. 2012. Pisolithus: a new species from southeast Asia and a new combination. Mycotaxon, Vol. 120, Issue. 1, p. 195.

Telleria, M. Teresa Dueñas, Margarita Melo, Ireneia Beltrán-Tejera, Esperanza Rodríguez-Armas, J. Laura Salcedo, Isabel and Martín, María P. 2012. Gloeocystidiellum kenyense in Azores and Madeira. Mycotaxon, Vol. 119, Issue. 1, p. 337.

Download full list

Article contents

Alternative Methods of extracting and Amplifying Dna from lichens

Abstract

Access options

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Article contents

Alternative Methods of extracting and Amplifying Dna from lichens

Abstract

Access options

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests