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Occurrence of high tyrosinase activity in the non-Peltigeralean lichen Dermatocarponminiatum (L.) W. Mann

Published online by Cambridge University Press:  08 October 2012

Richard P. BECKETT ,
Farida V. MINIBAYEVA  and
Christiane LIERS
Richard P. BECKETT
Affiliation:
School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg, Scottsville 3209, South Africa. Email: [email protected]
Farida V. MINIBAYEVA
Affiliation:
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P O Box 30, Kazan 420111, Russia
Christiane LIERS
Affiliation:
Department of Environmental Biotechnology, International Graduate School of Zittau, Markt 23, 02763 Zittau, Germany
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Abstract

In our earlier work, we demonstrated the presence of the multicopper oxidases tyrosinase and laccase in the cell walls of lichens from the Peltigerales, while these enzymes appeared to be absent in lichens from other orders. Likely roles for tyrosinase in lichens include melanin synthesis, the generation of quinones needed for laccase-mediated redox cycling, and the removal of harmful reactive molecules formed by this cycling. Non-Peltigeralean lichens will not need tyrosinase to detoxify laccase-generated radicals. However, many non-Peltigeralean lichens are often heavily melanized. Apparent absence of tyrosinase activity in these species prompted us to suggest that, in these lichens, melanins are probably synthesized by the polyketide pathway, which does not involve tyrosinase. Here, we surveyed intracellular tyrosinase activity in thallus homogenates from a range of lichens. Results showed that Peltigeralean species generally have much higher activities than species from other orders. However, the non-Peltigeralean lichen Dermatocarpon miniatum displays significant tyrosinase activity. In D. miniatum, tyrosinase differs from the corresponding enzyme from Peltigeralean lichens with respect to cellular location, substratum specificity, stability and pH optimum. Furthermore, unlike Peltigeralean lichens, in D. miniatum tyrosinase activity increased strongly following the rehydration of dry thalli. These differences are possibly a consequence of the role of tyrosinase in melanin synthesis rather than laccase-mediated redox cycling.

Keywords

dessicationlaccasemelaninphenol oxidasepolyketide
Type
Research Article
Information
The Lichenologist , Volume 44 , Issue 6 , November 2012 , pp. 827 - 832
Copyright
Copyright © British Lichen Society 2012

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References

Baldrian, P. (2006) Fungal laccases – occurrence and properties. FEMS Microbiology Reviews 30: 215–242.CrossRefGoogle Scholar
Beckett, R. P. & Minibayeva, F. V. (2007) Rapid breakdown of exogenous extracellular hydrogen peroxide by lichens. Physiologia Plantarum 129: 588–596.CrossRefGoogle Scholar
Bell, A. A. & Wheeler, M. H. (1986) Biosynthesis and functions of fungal melanin. Annual Review of Phytopathology 24: 411–451.CrossRefGoogle Scholar
Espìn, J. C. & Wichers, H. J. (1999) Activation of a latent mushroom (Agaricus bisporus) tyrosinase isoform by sodium dodecyl sulfate (SDS). Kinetic properties of the SDS-activated isoform. Journal of Agricultural and Food Chemistry 47: 3518–3525.CrossRefGoogle ScholarPubMed
Gómez-Toribio, V., García-Martín, A. B., Martínez, M. J., Martínez, Á. T. & Guillén, F. (2009 a) Enhancing the production of hydroxyl radicals by Pleurotus eryngii via quinone redox cycling for pollutant removal. Applied and Environmental Microbiology 75: 3954–3962.CrossRefGoogle ScholarPubMed
Gómez-Toribio, V., García-Martín, A. B., Martínez, M. J., Martínez, Á. T. & Guillén, F. (2009 b) Induction of extracellular hydroxyl radical production by white-rot fungi through quinone redox cycling. Applied and Environmental Microbiology 75: 3944–3953.CrossRefGoogle ScholarPubMed
Henson, J. M., Butler, M. J. & Day, A. W. (1999) The dark side of the mycelium: melanins of phytopathogenic fungi. Annual Review of Phytopathology 37: 447–471.CrossRefGoogle ScholarPubMed
Horowitz, N., Gling, M. & Horn, G. (1970) Tyrosinase (Neurospora crassa). Methods in Enzymology 17: 615–620.CrossRefGoogle Scholar
Larsson, P., Vecerova, K., Cempirkova, H., Solhaug, K. A. & Gauslaa, Y. (2009) Does UV-B influence biomass growth in lichens deficient in sun-screening pigments? Environmental and Experimental Botany 67: 215–221.CrossRefGoogle Scholar
Laufer, Z., Beckett, R. P. & Minibayeva, F. V. (2006 a) Co-occurrence of the multicopper oxidases tyrosinase and laccase in lichens in sub-order Peltigerineae. Annals of Botany 98: 1035–1042.CrossRefGoogle ScholarPubMed
Laufer, Z., Beckett, R. P., Minibayeva, F.V., Luthje, S. & Böttger, M. (2006 b) Occurrence of laccases in lichenized ascomycetes of the Peltigerineae. Mycological Research 110: 846–853.CrossRefGoogle ScholarPubMed
Liers, C., Ullrich, R., Hofrichter, M., Minibayeva, F. V. & Beckett, R. P. (2011) Oxidoreductases from lichenized ascomycetes: purification and characterization of a heme-peroxidase from Leptogium saturninum that oxidizes high-redox potential substrates. Fungal Genetics and Biology 48: 1139–1145.CrossRefGoogle Scholar
Lisov, A., Zavarzina, A., Zavarzin, A., Demin, V. & Leontievsky, A. (2012) Dimeric and monomeric laccases of soil-stabilizing lichen Solorina crocea: purification, properties and reactions with humic acids. Soil Biology and Biochemistry 45: 161–167.CrossRefGoogle Scholar
McEvoy, M., Gauslaa, Y. & Solhaug, K. A. (2007) Changes in pools of depsidones and melanins, and their function, during growth and acclimation under contrasting natural light in the lichen Lobaria pulmonaria. New Phytologist 175: 271–282.CrossRefGoogle ScholarPubMed
Muggia, L. & Grube, M. (2010) Type III polyketide synthases in lichen mycobionts. Fungal Biology 114: 379–385.Google ScholarPubMed
Muggia, L., Schmitt, I., & Grube, M. (2009) Lichens as treasure chests of natural products. SIM News 59: 85–97.Google Scholar
Nakamura, K. & Go, N. (2005) Function and molecular evolution of multicopper blue proteins. Cellular and Molecular Life Sciences 62: 2050–2066.CrossRefGoogle ScholarPubMed
Nybakken, L. & Julkunen-Tiitto, R. (2006) UV-B induces usnic acid in reindeer lichens. Lichenologist 38: 477–485.CrossRefGoogle Scholar
Nybakken, L., Asplund, J., Solhaug, K. A. & Gauslaa, Y. (2007) Forest successional stage affects the cortical secondary chemistry of three old forest lichens. Journal of Chemical Ecology 33: 1607–1618.CrossRefGoogle ScholarPubMed
Plonka, P. M. & Grabacka, M. (2006) Melanin synthesis in microorganisms - biotechnological and medical aspects. Acta Biochimica Polonica 53: 429–443.CrossRefGoogle ScholarPubMed
Ratcliffe, B., Flurkey, W. H., Kuglin, J. & Dawley, R. (1994) Tyrosinase, laccase, and peroxidase in mushrooms Agaricus, Crimini, Oyster and Shilitake. Journal of Food Science 59: 824–827.Google Scholar
Rodríguez-López, R. N., Escribano, J. & García-Cánovas, F. (1994) A continuous spectrophotometric method for the determination of monophenolase activity of tyrosinase using 3-methyl-2-benzothiazolinone hydrazone. Analytical Biochemistry 216: 205–212.CrossRefGoogle ScholarPubMed
Sinsabaugh, R. L. (2010) Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biology and Biochemistry 42: 391–404.CrossRefGoogle Scholar
Solomon, E. I., Chen, P., Metz, M., Lee, S. K. & Palmer, A. E. (2001) Oxygen binding, activation, and reduction to water by copper proteins. Angewandte Chemie 40: 4570–4590.3.0.CO;2-4>CrossRefGoogle ScholarPubMed
Zagoskina, H. B., Nikolaeva, T. H., Lapshin, P. V., Zavarzina, A. & Zavarzin, A. (2011) On the phenol composition of various species of lichens from the Kola Peninsula. Chemistry of Plant Raw Materials 4: 245–249.Google Scholar
Zavarzina, A. G. & Zavarzin, A. A. (2006) Laccase and tyrosinase activities in lichens. Microbiology 75: 546–556.CrossRefGoogle ScholarPubMed
Zavarzina, A. G., Lisov, A. A., Zavarzin, A. A. & Leontievsky, A. A. (2011) Fungal oxidoreductases and humification in forest soils. Soil Biology 22: 207–228.CrossRefGoogle Scholar