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Mass spectrometry as a versatile ancillary technique for the rapid in situ identification of lichen metabolites directly from TLC plates

Published online by Cambridge University Press:  19 September 2017

Pierre LE POGAM ,
Aline PILLOT ,
Françoise LOHEZIC-LE DEVEHAT ,
Anne-Cécile LE LAMER ,
Béatrice LEGOUIN ,
Alice GADEA ,
Aurélie SAUVAGER ,
Damien ERTZ  and
Joël BOUSTIE
Pierre LE POGAM
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected] Institute of Electronics and Telecommunications of Rennes, IETR, UMR CNRS 6164, University of Rennes 1, 263 Av. du Général Leclerc, Rennes Cedex 35042, France
Aline PILLOT
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected] UMR CNRS 6286, University of Nantes, 2 Rue de la Houssinière, Nantes Cedex 44322, France
Françoise LOHEZIC-LE DEVEHAT
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected]
Anne-Cécile LE LAMER
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected] University Paul Sabatier Toulouse 3, 118 Route de Narbonne, 31062 Toulouse, France
Béatrice LEGOUIN
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected]
Alice GADEA
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected] UMR CNRS 6553, ECOBIO, University of Rennes 1, 263 Av. du Général Leclerc, Rennes Cedex 35042, France
Aurélie SAUVAGER
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected]
Damien ERTZ
Affiliation:
Botanic Garden Meise, Department Bryophytes-Thallophytes (BT), Nieuwelaan 38, B-1860 Meise, Belgium
Joël BOUSTIE
Affiliation:
Institute of Chemistry of Rennes, ISCR, UMR CNRS 6226, University of Rennes 1, 2 Av. du Prof. Léon Bernard, Rennes Cedex 35043, France. Email: [email protected]
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Abstract

Thin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.

Keywords

acetone extractsanalytical techniqueschemical profilingUsnea trachycarpa
Type
Articles
Information
The Lichenologist , Volume 49 , Issue 5 , September 2017 , pp. 507 - 520
Copyright
© British Lichen Society, 2017 

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