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Four new epiphytic species in the Micarea prasina group from Europe

Published online by Cambridge University Press:  20 February 2019

Annina LAUNIS ,
Juha PYKÄLÄ ,
Pieter VAN DEN BOOM ,
Emmanuël SÉRUSIAUX  and
Leena MYLLYS
Annina LAUNIS
Affiliation:
Botany Unit, Finnish Museum of Natural History, P.O. Box 7, FI-00014 University of Helsinki, Finland. Email: [email protected]
Juha PYKÄLÄ
Affiliation:
Natural Environment Centre, Finnish Environment Institute, P.O. Box 140, FI-00251 Helsinki, Finland
Pieter VAN DEN BOOM
Affiliation:
Arafura 16, NL-5691 JA Son, The Netherlands
Emmanuël SÉRUSIAUX
Affiliation:
Evolution and Conservation Biology Unit, InBios Research Center, University of Liège, Sart Tilman B22, B-4000 Liège, Belgium
Leena MYLLYS
Affiliation:
Botany Unit, Finnish Museum of Natural History, P.O. Box 7, FI-00014 University of Helsinki, Finland. Email: [email protected]
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Abstract

In this study we clarify the phylogeny and reassess the current taxonomy of the Micarea prasina group, focusing especially on the M. byssacea and M. micrococca complexes. The phylogeny was investigated using ITS, mtSSU and Mcm7 regions from 25 taxa belonging to the M. prasina group. A total of 107 new sequences were generated. Data were analyzed using maximum parsimony and maximum likelihood methods. The results reveal five undescribed well-supported lineages. Four of the lineages represent new species described as Micarea pseudomicrococca Launis & Myllys sp. nov., M. czarnotae Launis, van den Boom, Sérusiaux & Myllys sp. nov., M. microareolata Launis, Pykälä & Myllys sp. nov. and M. laeta Launis & Myllys sp. nov. In addition, a fifth lineage was revealed that requires further study. Micarea pseudomicrococca is characterized by an olive green granular thallus, small cream-white or brownish apothecia lacking the Sedifolia-grey pigment and two types of paraphyses up to 2 µm wide. Micarea czarnotae forms a granular, densely granular or continuous olive green thallus, convex to hemispherical apothecia often with the Sedifolia-grey pigment and no crystalline granules in the thallus. Micarea microareolata is characterized by a ± pale green areolate thallus (composed of goniocysts), cream-white apothecia lacking the Sedifolia-grey pigment and narrow spores. Micarea laeta has a vivid to olive green granular thallus, pale apothecia lacking the Sedifolia-grey pigment and wider spores compared to M. microareolata. Descriptions, images and a key are provided for the new species. Crystalline granules are introduced as a novel species-level character for Micarea.

Keywords

crystalline granulesITSlichensMcm7mtSSUtaxonomy
Type
Articles
Information
The Lichenologist , Volume 51 , Issue 1 , January 2019 , pp. 7 - 25
Copyright
© British Lichen Society, 2019 

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References

Andersen, H. L. (2004) Phylogeny and classification of Micarea. Ph.D. thesis, University of Bergen.Google Scholar
Andersen, H. L. & Ekman, S. (2005) Disintegration of the Micareaceae (lichenized Ascomycota): a molecular phylogeny based on mitochondrial rDNA sequences. Mycological Research 109: 21–30.Google Scholar
Aptroot, A. & Cáceres, M. E. S. (2014) New lichen species from termite nests in rainforest in Brazilian Rondônia and adjacent Amazonas. Lichenologist 46: 365–372.Google Scholar
Barton, J. & Lendemer, J. C. (2014) Micarea micrococca and M. prasina, the first assessment of two very similar species in eastern North America. Bryologist 117: 223–231.Google Scholar
Brand, A. M., van den Boom, P. P. G. & Sérusiaux, E. (2014) Unveiling a surprising diversity in the lichen genus Micarea (Pilocarpaceae) in Réunion (Mascarenes archipelago, Indian Ocean). Lichenologist 46: 413–439.Google Scholar
Brodo, I. M. (1984) The North American species of the Lecanora subfusca group. Nova Hedwigia 79: 63–185.Google Scholar
Cáceres, M. E. S., Mota, D. A., de Jesus, L. S. & Aptroot, A. (2013) The new lichen species Micarea corallothallina from Serra da Jibóia, an Atlantic rainforest enclave in Bahia, NE Brazil. Lichenologist 45: 371–373.Google Scholar
Coppins, B. J. (1983) A taxonomic study of the lichen genus Micarea in Europe. Bulletin of the British Museum (Natural History), Botany Series 11: 17–214.Google Scholar
Coppins, B. J. (2002) Checklist of Lichens of Great Britain and Ireland. London: British Lichen Society.Google Scholar
Coppins, B. J. (2009) Micarea Fr. (1825). In The Lichens of Great Britain and Ireland (C. W. Smith, A. Aptroot, B. J. Coppins, A. Fletcher, O. L. Gilbert, P. W. James & P. A. Wolseley, eds): 583–606. London: British Lichen Society.Google Scholar
Coppins, B. J. & Tønsberg, T. (2001) A new xanthone-containing Micarea from Northwest Europe and the Pacific Northwest of North America. Lichenologist 33: 93–96.Google Scholar
Córdova-Chávez, O., Aptroot, A., Castillo-Camposa, G., Cáceres, M. E. S. & Pérez-Pérez, R. E. (2014) Three new lichen species from cloud forest in Veracruz, Mexico. Cryptogamie, Mycologie 35: 157–162.Google Scholar
Culberson, C. F. & Kristinsson, H. D. (1970) A standardized method for the identification of lichen products. Journal of Chromatography A 46: 85–93.Google Scholar
Czarnota, P. (2007) The lichen genus Micarea (Lecanorales, Ascomycota) in Poland. Polish Botanical Studies 23: 1–190.Google Scholar
Czarnota, P. & Guzow-Krzemińska, B. (2010) A phylogenetic study of the Micarea prasina group shows that Micarea micrococca includes three distinct lineages. Lichenologist 42: 7–21.Google Scholar
Edgar, R. C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797.Google Scholar
Ekman, S. & Svensson, M. (2014) Brianaria (Psoraceae), a new genus to accommodate the Micarea sylvicola group. Lichenologist 46: 285–294.Google Scholar
Goloboff, P., Farris, J. & Nixon, K. (2008) TNT, a free program for phylogenetic analysis. Cladistics 24: 774–786.Google Scholar
Guzow-Krzemińska, B., Czarnota, P., Łubek, A. & Kukwa, M. (2016) Micarea soralifera sp. nov., a new sorediate species in the M. prasina group. Lichenologist 48: 161–169.Google Scholar
Hafellner, J. (1984) Studien in Richtung einer natürlicheren Gliederung der Sammelfamilien Lecanoraceae und Lecideaceae . Beiheft zur Nova Hedwigia 79: 241–371.Google Scholar
Hedlund, J. T. (1892) Kritische Bemerkungen über einige Arten der Flechtengattungen Lecanora (Ach.), Lecidea (Ach.) und Micarea (Fr.). Bihang till Kongliga Svenska Vetenskaps-Akademiens Handlingar III 18: 1–104.Google Scholar
Kauff, F. & Lutzoni, F. (2002) Phylogeny of the Gyalectales and Ostropales (Ascomycota, Fungi): among and within order relationships based on nuclear ribosomal RNA small and large subunits. Molecular Phylogenetics and Evolution 25: 138–156.Google Scholar
Kirk, P. M., Cannon, P. F., Minter, D. W. & Stalpers, J. A. (2008) Ainsworth & Bisby’s Dictionary of the Fungi, 10th edition. Wallingford: CABI Publishing.Google Scholar
Launis, A. & Myllys, L. (2014) Micarea byssacea new to North America and M. hedlundii new to Maine, Michigan and Quebec. Opuscula Philolichenum 13: 84–90.Google Scholar
Leavitt, S. D., Johnson, L., Goward, T. & St. Clair, L. (2011) Species delimitation in taxonomically difficult lichen-forming fungi: an example from morphologically and chemically diverse Xanthoparmelia (Parmeliaceae) in North America. Molecular Phylogenetics and Evolution 60: 317–332.Google Scholar
Lumbsch, H. T. & Leavitt, S. D. (2011) Goodbye morphology? A paradigm shift in the delimitation of species in lichenized fungi. Fungal Diversity 50: 59–72.Google Scholar
Mark, K., Saag, L., Leavitt, S. D., Will-Wolf, S., Nelsen, M. P., Tõrra, T., Saag, A., Randlane, T. & Lumbsch, H. T. (2016) Evaluation of traditionally circumscribed species in the lichen-forming genus Usnea, section Usnea (Parmeliaceae, Ascomycota) using a six-locus dataset. Organisms Diversity and Evolution 16: 497–524.Google Scholar
Maddison, D. R. & Maddison, W. P. (2005) MacClade 4: Analysis of Phylogeny and Character Evolution. Sunderland, Massachusetts: Sinauer Associates.Google Scholar
McCarthy, P. M. & Elix, J. A. (2016) A new species of Micarea (lichenized Ascomycota, Pilocarpaceae) from alpine Australia. Telopea 19: 31–35.Google Scholar
Meyer, B. & Printzen, C. (2000) Proposal for a standardized nomenclature and characterization of insoluble lichen pigments. Lichenologist 32: 571–583.Google Scholar
Myllys, L., Lohtander, K., Källersjö, M. & Tehler, A. (1999) Sequence insertion and ITS data provide congruent information in Roccella canariensis and R. tuberculata (Arthoniales, Euascomycetes) phylogeny. Molecular Phylogenetics and Evolution 12: 295–309.Google Scholar
Myllys, L., Velmala, S., Holien, H., Halonen, P., Wang, L. S. & Goward, T. (2011) Phylogeny of the genus Bryoria . Lichenologist 43: 617–638.Google Scholar
Orange, A., James, P. W. & White, F. J. (2010) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.Google Scholar
Schmitt, I., Crespo, A., Divakar, P. K., Fankhauser, J. D., Herman-Sackett, E., Kalb, K., Nelsen, M. P., Nelson, N. A., Rivas-Plata, E., Shimp, A. D., et al. (2009) New primers for promising single-copy genes in fungal phylogenetics and systematics. Persoonia 23: 35–40.Google Scholar
Schmull, M., Miadlikowska, J., Pelzer, M., Stocker-Wörgötter, E., Hofstetter, V., Fraker, E., Hodkinson, B., Reeb, V., Kukwa, M., Lumbsch, H. T., et al. (2011) Phylogenetic affiliations of members of the heterogeneous lichen-forming fungi of the genus Lecidea sensu Zahlbruckner (Lecanoromycetes, Ascomycota). Mycologia 103: 983–1003.Google Scholar
Sérusiaux, E., Brand, A. M., Motiejūnaitè, J., Orange, A. & Coppins, B. J. (2010) Lecidea doliiformis belongs to Micarea, Catillaria alba to Biatora and Biatora ligni-mollis occurs in Western Europe. Bryologist 113: 333–344.Google Scholar
Spribille, T., Klug, B. & Mayrhofer, H. (2011) A phylogenetic analysis of the boreal lichen Mycoblastus sanguinarius (Mycoblastaceae, lichenized Ascomycota) reveals cryptic clades correlated with fatty acid profiles. Molecular Phylogenetics and Evolution 59: 603–614.Google Scholar
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313.Google Scholar
Svensson, M. & Thor, G. (2011) Micarea capitata, a new bryophilous lichen from Sweden. Lichenologist 43: 401–405.Google Scholar
van den Boom, P. P. G. & Coppins, B. J. (2001) Micarea viridileprosa sp. nov., an overlooked lichen species from Western Europe. Lichenologist 33: 87–91.Google Scholar
van den Boom, P. P. G. & Ertz, D. (2014) A new species of Micarea (Pilocarpacea) from Madeira growing on Usnea . Lichenologist 46: 295–301.Google Scholar
van den Boom, P. P. G., Brand, A. M., Coppins, B. J. & Sérusiaux, E. (2017) Two new species in the Micarea prasina group from Western Europe. Lichenologist 49: 13–25.Google Scholar
White, T. J., Bruns, T., Lee, S. & Taylor, J. W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315–322. New York: Academic Press.Google Scholar
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming Ascomycetes. Lichenologist 31: 511–516.Google Scholar