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X-ray microscopy of living multicellular organisms with the Prague Asterix Iodine Laser System

Published online by Cambridge University Press:  25 March 2004

T. DESAI ,
D. BATANI ,
A. BERNARDINELLO ,
G. POLETTI ,
F. ORSINI ,
J. ULLSCHMIED ,
L. JUHA ,
J. SKALA ,
B. KRALIKOVA  and
E. KROUSKY
...Show all authors
T. DESAI
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
D. BATANI
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
A. BERNARDINELLO
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
G. POLETTI
Affiliation:
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Milano, Italy
F. ORSINI
Affiliation:
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Milano, Italy
J. ULLSCHMIED
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
L. JUHA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
J. SKALA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
B. KRALIKOVA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
E. KROUSKY
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
M. PFEIFER
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
C. KADLEC
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
T. MOCEK
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
A. PRÄG
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
O. RENNER
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
F. COTELLI
Affiliation:
Dipartimento di Biologia, Università di Milano, Milano, Italy
C. LORA LAMIA
Affiliation:
Dipartimento di Biologia, Università di Milano, Milano, Italy
A. ZULLINI
Affiliation:
Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Milano, Italy
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Abstract

Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were λ = 1.314 μm and τ (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I ≥ 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.

Keywords

Atomic force microscopyLaser-produced plasmasMulticellular Caenorhabditis elegansSoft X-ray contact microscopy
Type
Research Article
Information
Laser and Particle Beams , Volume 21 , Issue 4 , October 2003 , pp. 511 - 516
Copyright
© 2003 Cambridge University Press

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