The echolocation calls of insectivorous bats of the northern Yucatan Peninsula, Mexico, with the exception of the phyllostomids and molossids, are presented. The aim is to provide a catalogue of bat sounds that can be used for acoustic inventories of insectivorous bats using the Pettersson heterodyne and time-expansion bat detectors. The acoustic method can be used alone or in combination with inventories based on mist-netting, a method more suitable for the low-intensity echolocators (mainly the phyllostomids), which are difficult to monitor acoustically. The insectivorous species of the Yucatan are generally easy to identify by their echolocation calls, particularly when combined with visual observations of foraging bats at dusk.