Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-18T01:26:21.315Z Has data issue: false hasContentIssue false
  • English
  • Français

Rapid extraction of invertebrates from tropical forest litter using modified Winkler apparatus

Published online by Cambridge University Press:  04 December 2014

Eugenia E. Semenina ,
Alexander E. Anichkin ,
Oksana L. Shilenkova ,
Sergey G. Ermilov  and
Alexei V. Tiunov
Eugenia E. Semenina
Affiliation:
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia
Alexander E. Anichkin
Affiliation:
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia The Russian-Vietnamese Tropical Scientific and Technological Centre, Southern Branch, Ho Chi Minh, Vietnam
Oksana L. Shilenkova
Affiliation:
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia
Sergey G. Ermilov
Affiliation:
Tyumen State University, Semakova Street 10, 625003 Tyumen, Russia
Alexei V. Tiunov*
Affiliation:
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia
*
1Corresponding author. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract:

The Winkler extractor is widely used for collecting both micro- and macrofauna from large samples of forest litter, but the long period of extraction that it demands and that allows continuous feeding and reproduction of target organisms is a serious drawback when samples are collected for subsequent stable isotope analysis. This problem is exacerbated in a humid tropical climate. In this study, we assessed the effectiveness of the modified Winkler apparatus equipped with heating plates placed inside each mesh bag. The study was performed at the end of the wet season in a mature forest and young forest plantation at Cat Tien National Park, southern Vietnam. The heating plates increased the temperature within the extractors by about 8ºC and accelerated the desiccation of litter in the mesh bags. The extraction speed of macrofauna from the forest litter was strongly increased and the number of mites collected nearly doubled in the modified extractors, though the extraction rates of collembolans were not affected significantly. Overall, our results suggest that the application of heating plates accelerates the extraction process while yielding large quantities of litter-dwelling animals.

Keywords

collembolansheating platesmicro-arthropodsmitesmonsoon tropical foreststable isotope analysissoil animals
Type
Short Communication
Information
Journal of Tropical Ecology , Volume 31 , Issue 2 , March 2015 , pp. 191 - 194
Copyright
Copyright © Cambridge University Press 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

LITERATURE CITED

ANICHKIN, A. E., BELYAEVA, N. V., DOVGOBROD, I. G., SHVEENKOVA, Y. B. & TIUNOV, A. V. 2007. Soil microarthropods and macrofauna in monsoon tropical forests of Cat Tien and Bi Dup-Nui Ba National Parks, southern Vietnam. Biology Bulletin 34:498–506.Google Scholar
BLANC, L., MAURY-LECHON, J. & PASCAL, J. P. 2000. Structure, floristic composition and natural regeneration in the forests of Cat Tien National Park, Vietnam: an analysis of the successional trends. Journal of Biogeography 27:141–157.Google Scholar
DELSINNE, T. D. & ARIAS-PENNA, T. M. 2012. Influence of leaf litter moisture on the efficiency of the Winkler method for extracting ants. Journal of Insect Science 12:1–13.Google Scholar
DESHCHEREVSKAYA, O. A., AVILOV, V. K., DINH BA DUY, TRAN CONG HUAN & KURBATOVA, J. A. 2013. Modern climate of Cat Tien National park (Southern Vietnam): climatological data for ecological studies. Izvestiya, Atmospheric and Oceanic Physics 49:819–838.Google Scholar
ILLIG, J., LANGEL, R., NORTON, R. A., SCHEU, S. & MARAUN, M. 2005. Where are the decomposers? Uncovering the soil food web of a tropical montane rain forest in southern Ecuador using stable isotopes (15N). Journal of Tropical Ecology 21:589–593.Google Scholar
KRELL, F. T., CHUNG, A. Y. C., DEBOISEA, E., EGGLETON, P., GIUSTIA, A., INWARDA, K. & KRELL-WESTERWALBESLOH, S. 2005. Quantitative extraction of macro-invertebrates from temperate and tropical leaf litter and soil: efficiency and time-dependent taxonomic biases of the Winkler extraction. Pedobiologia 49:175–186.CrossRefGoogle Scholar
LARSEN, T., VENTURA, M., DAMGAARD, C., HOBBIE, E. A. & KROGH, P. H. 2009. Nutrient allocations and metabolism in two collembolans with contrasting reproduction and growth strategies. Functional Ecology 23:745–755.Google Scholar
LAVELLE, P. & SPAIN, A. V. 2001. Soil ecology. Kluwer Academic Publishers, Dordrecht. 654 pp.CrossRefGoogle Scholar
MARAUN, M., ERDMANN, G., FISCHER, B. M., POLLIERER, M. M., NORTON, R. A., SCHNEIDER, K. & SCHEU, S. 2011. Stable isotopes revisited: their use and limits for oribatid mite trophic ecology. Soil Biology and Biochemistry 43:877–882.Google Scholar
POTAPOV, A. M., SEMENINA, E. E., KURAKOV, A. V. & TIUNOV, A. V. 2013. Large 13C/12C and small 15N/14N isotope fractionation in an experimental detrital foodweb (litter–fungi–collembolans). Ecological Research 28:1069–1079.Google Scholar
SAKCHOOWONG, W., NOMURA, S., OGATA, K. & CHANPAISAENG, J. 2007. Comparison of extraction efficiency between Winkler and Tullgren extractors for tropical leaf litter macroarthropods. Thai Journal of Agricultural Science 40:97–105.Google Scholar
SEMENYUK, I. I., TIUNOV, A. V. & GOLOVATCH, S. I. 2011. Structure of mandibles in relation to trophic niche differentiation in a tropical millipede community. International Journal of Myriapodology 6:37–49.Google Scholar
SHAW, P. & OZANNE, C. 2011. A calibration of the efficiency of Winkler eclectors for extracting Collembola at different humidities. Soil Organisms 83:515–520.Google Scholar