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Genetic diversity of Philippine Rubus moluccanus L. (Rosaceae) populations examined with VNTR DNA probes

Published online by Cambridge University Press:  10 July 2009

Daniel T. Busemeyer ,
Stephan Pelikan ,
Robert S. Kennedy  and
Steven H. Rogstad
Daniel T. Busemeyer
Affiliation:
Department of Biological Sciences ML6, University of Cincinnati, Cincinnati, Ohio 45221–0006, U.S.A.
Stephan Pelikan
Affiliation:
Department of Mathematical Sciences ML25, University of Cincinnati, Cincinnati, Ohio 45221–0025, U.S.A.
Robert S. Kennedy
Affiliation:
Museum of Natural History & Science, Cincinnati Museum Center, 1720 Gilbert Ave., Cincinnati, Ohio 45202, U.S.A.
Steven H. Rogstad
Affiliation:
Department of Biological Sciences ML6, University of Cincinnati, Cincinnati, Ohio 45221–0006, U.S.A.
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Abstract

Two synthetic DNA probes composed of tandemly repeated ‘core’ sequences (GACA and GATA) were used to examine genetic diversity at multiple variable-number-tandem-repeat (VNTR) loci within and among four spatially isolated Philippine populations of Rubus moluccanus L. (Rosaceae), an uncultivated bramble widely distributed throughout southeast Asia – Malesia. Central goals were to determine whether apomictic propagation was detectable in R. moluccanus and to examine whether populations isolated on separate islands, or on mountain tops on a single island, were genetically differentiated. Sampling 22 individuals per population, the findings include: (1) no two individuals shared identical VNTR band profiles and thus apomictic propagation was not detected; (2) the mean number of bands scored per individual was 24.3 (SD = 3.91) and the proportion of polymorphic loci within populations ranged from 0.69 to 1.00 (mean = 0.86 ± SD = 0.085); (3) the average proportion of bands shared between individuals within populations ranged from 0.39 to 0.67 (0.50 ± 0.067), while average interpopulation similarity ranged from 0.21 to 0.50 (0.32 ± 0.092); and (4) estimated heterozygosity within populations ranged from 0.42 to 0.79 (0.62 ± 0.083), while interpopulation heterozygosity ran from 0.62 to 0.81 (0.74 ± 0.062). Permutation tests were used to estimate the statistical significance of differences in similarity between populations. A Luzon population isolated by 1200 km was always significantly different in similarity tests when compared with each of the other three populations, all located on Mindanao (and separated by at least 100 km but less than 250 km). Of the latter three, only one population differed significantly from the other two in the degree of VNTR markers shared, possibly reflecting biogeographic partitioning suggested for the island. Across all populations, average estimated Fst was 0.154, although mean inter-island Fst (0.224) was significantly higher than mean intra-island Fst (0.085). These results suggest that, while intra-island gene flow is relatively high, significant differentiation of tropical species may occur even over short distances on individual islands. Preservation of only a limited number of populations may result in a significant loss of genetic diversity in such species.

Keywords

conservation biologyDNA fingerprintingminisatellite DNApopulation geneticsrflp analysis
Type
Research Article
Information
Journal of Tropical Ecology , Volume 13 , Issue 6 , November 1997 , pp. 867 - 884
Copyright
Copyright © Cambridge University Press 1997

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