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The use of thin-layer chromatography for the separation and identification of phytoplankton pigments

Published online by Cambridge University Press:  11 May 2009

J. P. Riley  and
T. R. S. Wilson
J. P. Riley
Affiliation:
Department of Oceanography, The University of Liverpool, Liverpool 3
T. R. S. Wilson
Affiliation:
Department of Oceanography, The University of Liverpool, Liverpool 3
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Extract

Thin-layer chromatography has been used for the rapid separation of microgram amounts of pigments of marine phytoplankton, both from cultures and from the sea. The separation is carried out on plates coated with silica gel; the chromatogram is developed with a solvent consisting of petroleum ether (b.p. 60–80°), ethyl acetate and diethylamine in the ratio 58:30:12 by volume. The separated individual pigments may be eluted from the plate and characterized by their absorption spectra. The pigments of the following phytoplankton have been examined: Dunaliella primolecta, Phaeodactylum tricornutum, Isochrysis galbana, Dicrateria inornata, Coccolithus huxleyi, and Peridinium trochoidium.

Introduction

A knowledge of the component pigments of phytoplankton is of value in the systematic classification of these organisms and in the study of the mechanism of photosynthesis. Very little is known about the amounts of the lesser phyto-plankton pigments associated with the natural phytoplankton crop in the sea, but a considerable amount of work has been carried out on the measurement of chlorophylls a, b and c and of carotenoids for the estimation of the plant bio-mass in the sea. These determinations are usually carried out by modifications of the poly-chromatic spectrophotometric method originally described by Richards with Thompson (1952) and modified by Creitz & Richards (1955) (see also Parsons & Strickland, 1963). While these procedures give a satisfactory measure of chlorophylls a and b, their precision for chlorophyll c and carotenoids is poor; furthermore, no resolution of carotenes from xanthophylls can be attained; nor can any indication of the presence of unusual pigments be obtained.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 45 , Issue 3 , October 1965 , pp. 583 - 591
Copyright
Copyright © Marine Biological Association of the United Kingdom 1965

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References

Bacon, M. F., 1965. Separation of chlorophylls a and b and related compounds by thin layer chromatography on cellulose. J. Chromat., Vol. 17, No. 2, pp. 322–6.CrossRefGoogle Scholar
Barrett, J. & Jeffrey, S. W., 1964. Chlorophyllase and formation of an atypical chlorophyllide in marine algae. Plant Physiol. Lancaster, Vol. 39, pp. 44–67.CrossRefGoogle ScholarPubMed
Colman, B. & Vishniac, W., 1964. Separation of chloroplast pigments on thin layers of sucrose. Biochim. biophys. Acta, Vol. 82, pp. 616–18.CrossRefGoogle ScholarPubMed
Creitz, , Grace, I. & Richards, F. A., 1955. The estimation and characterization of plankton populations by pigment analysis. III. A note on the use of ‘Millipore’ membrane niters in the estimation of plankton pigments. J. mar. Res., Vol. 14, pp. 211–16.Google Scholar
Dales, R. P., 1960. On the pigments of the Chrysophyceae. J. mar. biol. Ass. U.K., Vol. 39, pp. 693–99.CrossRefGoogle Scholar
Dallas, M. S. J., 1965. Reproducible R f values in thin layer absorption chromatography. J. Chromat., Vol. 17, No. 2, pp. 267–77.CrossRefGoogle Scholar
Gilchrist, B. M. & Green, J., 1960. The pigments of Anemia. Proc. R. Soc, B, Vol. 152, pp. 118–36.Google Scholar
Jeffrey, S. W., 1961. Paper chromatographic separation of chlorophylls and carotenoids from marine algae. Biochem. J., Vol. 80, pp. 336–42.CrossRefGoogle ScholarPubMed
Jeffrey, S. W. & Allen, M. B., 1964. Pigments, growth and photosynthesis in cultures of two Chrysomonads, Coccolithus huxleyi and Hymenomonas sp. J. gen. Microbiol., Vol. 36, pp. 277–88.CrossRefGoogle ScholarPubMed
Parke, M. & Dixon, P. S., 1964. A revised check list of British Marine Algae. J. mar. biol. Ass. U.K., Vol. 44, pp. 499–542.CrossRefGoogle Scholar
Parsons, T. R., 1961. On the pigment composition of eleven species of marine phytoplankters. J. Fish. Res. Bd Can., Vol. 18, pp. 1017–25.CrossRefGoogle Scholar
Parsons, T. R. & Strickland, J. D. H., 1963. Discussion of spectrophotometric determination of marine plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. J. mar. Res., Vol. 21, pp. 155–63.Google Scholar
Richards, F. A. with Thompson, T. G., 1952. The estimation and characterization of plankton populations by pigment analysis. II. A spectrophotometric method for the estimation of plankton pigments. J. mar. Res., Vol. 2, pp. 150–72.Google Scholar
Šesták, Z., 1958 Paper chromatography of chloroplast pigments. J. Chromat., Vol. 1, pp. 293–308.CrossRefGoogle Scholar
Spencer, C. P., 1964. The estimation of phytoplankton pigments. J. Cons. perm. int. Explor. Mer, Vol. 28, pp. 327–34.CrossRefGoogle Scholar
Strain, H. H., 1958. Chloroplast pigments and chromatographic analysis. 32nd annual Priestley Lecture. University Park, Pennsylvania.Google Scholar
Strain, H. H., Manning, W. M. & Hardin, G., 1944. Xanthophylls and carotenes of diatoms, brown algae, dinoflagellates and sea anemones. Biol. Bull. mar. biol. Lab., Woods Hole, Vol. 86, pp. 169–91.CrossRefGoogle Scholar
Yentsch, C. S. & Menzel, D. W. (1963). A method for the determination of phyto-plankton chlorophyll and phaeophytin by fluorescence. Deep-Sea Res., Vol. 10, pp. 221–31.Google Scholar