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A suggested method for the assay of vitamin B12 in sea water

Published online by Cambridge University Press:  11 May 2009

M. R. Droop
M. R. Droop
Affiliation:
Marine Station, Millport
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Extract

One of the difficulties encountered in the use of fresh water organisms for the assay of vitamin B12 of marine origin is the elimination of salt from the extracts. This has suggested that a marine organism might be used with advantage. Indeed on several occasions marine or supra-littoral species have been proposed as possible assay subjects, but acceptable techniques await development (Lewin, 1954; Droop, 1954; Sweeney, 1954). Monochrysis lutheri, a supra-littoral euryhaline species, has advantages over others in being small, robust and giving heavy yields in synthetic media. The method employed in some Monochrysis assays carried out in the early spring of 1954 will be described.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 34 , Issue 3 , October 1955 , pp. 435 - 440
Copyright
Copyright © Marine Biological Association of the United Kingdom 1955

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References

Droop, M. R., 1954. Cobalamin requirement in Chrysophyceae. Nature, Lond., Vol. 174, p. 520.CrossRefGoogle Scholar
Droop, M. R.,1955. Some new supra-littoral Protista. J. Mar. biol. Ass. U.K., Vol. 34, pp. 233–45.CrossRefGoogle Scholar
Finney, D. J., 1952. Statistical Method in Biological Assay. London, Charles Griffin and Co.Google Scholar
Lewin, R. A., 1954. A marine Stichococcus sp. which requires vitamin B12 (cobalamin). J. Gen. Microbiol., Vol. 10, pp. 93–6.CrossRefGoogle ScholarPubMed
Provasoli, L. & Pintner, I. J., 1953. Assay of vitamin B12 in sea water. Proc. Soc. Protozool., Vol. 4, p. 10.Google Scholar
Sweeney, B. M., 1954. Gymnodiniwn splendens, a marine dinoflagellate requiring vitamin B12. Amer.J. Bot., Vol. 41, pp. 821–4.CrossRefGoogle Scholar