By estimating samples of diatoms taken from 0.2 m contour lines, over a horizontal range of about 85 m in the months of July 1953 a nd February 1954 the ecological factors influencing the distribution of eleven species of mud-flat diatoms were investigated. The three important factors were considered to be: the resistance to desiccation near the M.H.W.L., the ability to live under short periods of illumination near to the M.L.W.L., and the ability to tolerate the organic materials present in the black sulphureous layer. Table 7 summarizes the results for the eleven species.