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Studies on benzo[a]pyrene mono-oxygenase in the shore crab Carcinus maenas

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
E. D. S. Corner ,
T. E. V. Forsberg  and
M. N. Moore
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
T. E. V. Forsberg
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
M. N. Moore
Affiliation:
Natural Environment Research Council, Institute for Marine Environmental Research, Prospect Place, The Hoe, Plymouth, PL1 3DH
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Extract

A cytochrome P-450-dependent benzo[a]pyrene mono-oxygenase enzyme system (BPM) has been identified and partially characterized in males of the shore crab Carcinus maenas (L.). Apparent Km values obtained at 30 °C using microsomal preparations from the antennary glands of animals collected during summer were in the range 1.61–2.11 µM. The cytochrome P-450 content was 0·022 nmol/mg microsomal protein when BPM activity in the same preparation was 0·085 nmol/mg protein/min.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 62 , Issue 2 , May 1982 , pp. 339 - 357
Copyright
Copyright © Marine Biological Association of the United Kingdom 1982

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