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Sotalia dolphins in their potential sympatry zone: searching for hybrids in the Amazonian estuary

Published online by Cambridge University Press:  02 July 2018

Teresa E.C. Dos Santos ,
Vera M.F. Da Silva ,
Nívia A.S. Do Carmo ,
Cristiano Lazoski  and
Haydée A. Cunha
Teresa E.C. Dos Santos
Affiliation:
Programa de Pós-Graduação em Biodiversidade e Biologia Evolutiva, Universidade Federal do Rio de Janeiro, Interbloco B/C do Centro de Ciências da Saúde. Ilha do Fundão, Rio de Janeiro, Rio de Janeiro State, Cep 21941-590, Brazil
Vera M.F. Da Silva
Affiliation:
Laboratório de Mamíferos Aquáticos, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936. Petrópolis, Manaus, Amazonas State, Cep 69011-970, Brazil
Nívia A.S. Do Carmo
Affiliation:
Laboratório de Mamíferos Aquáticos, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936. Petrópolis, Manaus, Amazonas State, Cep 69011-970, Brazil
Cristiano Lazoski
Affiliation:
Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Prédio do CCS, Bl. A. Ilha do Fundão, Rio de Janeiro, Rio de Janeiro State, Cep 21941-590, Brazil
Haydée A. Cunha*
Affiliation:
Laboratório de Biodiversidade Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Prédio do CCS, Bl. A, Sl. A2-98. Ilha do Fundão, Rio de Janeiro, Rio de Janeiro State, Cep 21941-590, Brazil Laboratório de Mamíferos Aquáticos e Bioindicadores, Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro, R. São Francisco Xavier, 524. 4 andar, Bl. E, Sl. 4002-E. Maracanã, Rio de Janeiro, Rio de Janeiro State, Cep 20550-013, Brazil
*
Correspondence should be addressed to: Haydée A. Cunha, Laboratório de Mamíferos Aquáticos e Bioindicadores, Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro, R. São Francisco Xavier, 524. 4 andar, Bl. E, Sl. 4002-E. Maracanã, Rio de Janeiro, Rio de Janeiro State, Cep 20550-013, Brazil email: [email protected]
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Abstract

The two Sotalia species (the marine S. guianensis and the freshwater S. fluviatilis) have only recently been recognized, and both face several conservation challenges. We investigated the existence of hybridization between the two species in their possible area of sympatry in the Amazon Estuary, in northern Brazil. A fast and cheap PCR-RFLP diagnostic method using nuclear DNA was developed to discriminate between the two species, while allowing the detection of hybrids. All samples that could be identified (N = 51) were identified as S. guianensis, and no hybrids were detected. Our results, coupled with previous mitochondrial data, suggest that S. fluviatilis is not present in the Amazon delta. Thus, sympatry with S. guianensis, if it does occur, may be restricted to upstream areas of the Amazon River.

Keywords

Guiana dolphinforensicsintrogressiontucuxi
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 98 , Special Issue 5: Marine Mammals , August 2018 , pp. 1211 - 1215
Copyright
Copyright © Marine Biological Association of the United Kingdom 2018 

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References

REFERENCES

Baird, R.W., Willis, P.M., Guenther, T.J., Wilson, P.J. and White, B.N. (1998) An intergeneric hybrid in the family Phocoenidae. Canadian Journal of Zoology 76, 198–204.Google Scholar
Bérubé, M. and Aguilar, A. (1998) A new hybrid between a blue whale, Balaenoptera musculus, and a fin whale, Balaenoptera physalus: frequency and implications of hybridization. Marine Mammal Science 14, 82–98.Google Scholar
Caballero, S. and Baker, C.S. (2010) Captive-born intergeneric hybrid of a Guiana and bottlenose dolphin: Sotalia guianensis × Tursiops truncatus. ZooBiology 29, 647–657.Google Scholar
Caballero, S., Trujillo, F., Vianna, J.A., Barrios-Garrido, H., Montiel, M.G., Beltrán-Pedreros, S., Marmontel, M., Santos, M.C., Rossi-Santos, M., Santos, F.R. and Baker, C.S. (2007) Taxonomic status of the genus Sotalia: species level ranking for “tucuxi” (Sotalia fluviatilis) and “costero” (Sotalia guianensis) dolphins. Marine Mammal Science 23, 358–386.Google Scholar
Cunha, H.A., da Silva, V.M.F., Lailson-Brito, J. Jr, Santos, M.C.O., Flores, P.A.C., Martin, A.R., Azevedo, A.F., Fragoso, A.B.L., Zanelatto, R.C. and Solé-Cava, A.M. (2005) Riverine and marine ecotypes of Sotalia dolphins are different species. Marine Biology 148, 449–457.Google Scholar
Cunha, H.A., da Silva, V.M.F., Santos, T.E.C., do Carmo, N.A.S. and Solé-Cava, A.M. Submitted. Sex, lies and DNA: fraud in the illegal trade of Amazon River dolphin love charms.Google Scholar
Cunha, H.A., da Silva, V.M.F. and Solé-Cava, A.M. (2010) Molecular ecology and systematics of Sotalia dolphins. In Ruiz-Garcia, M. and Shostell, J.M. (eds) Biology, evolution and conservation of river dolphins within South America and Asia. New York, NY: Nova Science, pp. 261–283.Google Scholar
Cunha, H.A., Moraes, L.C., Medeiros, B.V., Lailson-Brito, J.J.R., da Silva, V.M.F., Solé-Cava, A.M. and Schrago, C.G. (2011) Phylogenetic status and timescale for the diversification of Steno and Sotalia dolphins. PLoS ONE 6, e28297–e28297.Google Scholar
Cunha, H.A. and Solé-Cava, A.M. (2007) Molecular sexing of tucuxi dolphins (Sotalia guianensis and Sotalia fluviatilis), using samples from biopsy darting and decomposed carcasses. Genetics and Molecular Biology 30, 1186–1188.Google Scholar
da Silva, V.M.F. and Best, R.C. (1996) Sotalia fluviatilis. Mammalian Species 527, 1–7.Google Scholar
da Silva, V.M.F., Fetuccia, D., Rodrigues, E.S., Edwards, H., Moreno, I.B., Moura, J.F., Wedekin, L.L., Bazzalo, M., Emin-Lima, N.R., do Carmo, N.A.S., Siciliano, S. and Utreras, V.B. (2010) Report of the working group on distribution, habitat characteristics and preferences, and group size. Latin American Journal of Aquatic Mammals 8, 31–38.Google Scholar
Dohl, T.P., Norris, K. and Kang, I. (1974) A porpoise hybrid: Tursiops × Steno. Journal of Mammalogy 55, 217–221.Google Scholar
Domning, D. (1981) Distribution and status of manatees Trichechus spp. near the mouth of the Amazon River, Brazil. Biological Conservation 19, 85–97.Google Scholar
Foote, A.D., Thomsen, P.F., Sveegaard, S., Wahlberg, M., Kielgast, J., Kyhn, L.A., Salling, A.B., Galatius, A., Orlando, L. and Gilbert, M.T.P. (2012) Investigating the potential use of environmental DNA (eDNA) for genetic monitoring of marine mammals. PLoS ONE 7, e41781. doi: 10.1371/journal.pone.0041781.Google Scholar
Goulding, M., Barthem, R., and Ferreira, E.J.G. (2003) The Smithsonian atlas of the Amazon. Washington, DC: Smithsonian Books. 253 pp.Google Scholar
Gravena, W., Hrbek, T., da Silva, V.M.F. and Farias, I.P. (2008) Amazon River dolphin love fetishes: from folklore to molecular forensics. Marine Mammal Science 24, 969–978.Google Scholar
Milinkovitch, M.C., Bérubé, M. and Palsbøll, P.J. (1998) Cetaceans are highly specialized artiodactyls. In Thewissen, J.G.M. (ed.) The emergence of whales: evolutionary patterns in the origin of Cetacea. New York, NY: Plenum, pp. 113–131.Google Scholar
MMA – Ministério de Meio Ambiente do Brasil (2014) Lista Nacional Oficial de Espécies da Fauna Ameaçadas de Extinção. Diário Oficial da União de 18 de dezembro de 2014, n. 245, Seção 1: 121–126. http://portal.imprensanacional.gov.br/acervo-dou/2014/dez/18.Google Scholar
Monteiro-Filho, E.L.A., Monteiro, L.R. and Reis, S.F. (2002) Skull shape and size divergence in dolphins of the genus Sotalia: a tridimensional morphometric analysis. Journal of Mammalogy 83, 125–134.Google Scholar
Muller-Karger, F.E., McClain, C.R. and Richardson, P.L., (1988) The dispersal of the Amazon's water. Nature 333, 57–59.Google Scholar
Nishiwaki, M. and Tobayama, T. (1982) Morphological study on the hybrid between Tursiops and Pseudorca. Scientific Reports of the Whales Research Institute 34, 109–121.Google Scholar
Reeves, R.R., Smith, B.D., Crespo, E.A. and Notarbartolo di Sciara, G. (2003) Dolphins, whales and porpoises: 2002–2010 conservation action plan for the world's cetaceans. Gland and Cambridge: IUCN/SSC Cetacean Specialist Group.Google Scholar
Roca, A.L., Georgiadis, N., Pecon-Slattery, J. and O'Brien, S.J. (2001) Genetic evidence for two species of elephant in Africa. Science 293, 1473–1477.Google Scholar
Ruiz-Garcia, M., Mejia, D., Escobar-Armel, P., Tejada-Martínez, D. and Shostell, J.M. (2013) Molecular identification and historic demography of the marine tucuxi (Sotalia guianensis) at the Amazon River’s mouth by means of mitochondrial control region gene sequences and implications for conservation. Diversity 5, 703–723.Google Scholar
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn. New York, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Secchi, E. (2012) Sotalia fluviatilis. The IUCN Red List of threatened species 2012: e.T190871A17583369. http://dx.doi.org/10.2305/IUCN.UK.2012.RLTS.T190871A17583369.en.Google Scholar
Sholl, T.G.C., Nascimento, F.F., Leoncini, O., Bonvicino, C.R. and Siciliano, S. (2008) Taxonomic identification of dolphin love charms commercialized in the Amazonian region through the analysis of cytochrome b DNA. Journal of the Marine Biological Association of the United Kingdom 88, 1207–1210.Google Scholar
Siciliano, S., Valiati, V.H., Emin-Lima, R., Costa, A.F., Sartor, J., Dorneles, T., Silva, J.S. and Oliveira, L.R. (2016) New genetic data extend the range of the river dolphins Inia in the Amazon Delta. Hydrobiologia 777, 255–269.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Vianna, J.A., Bonde, R.K., Caballero, S., Giraldo, J.P., Lima, R.P., Clark, A., Marmontel, M., Morales-Vela, B., Souza, M.J.D., Parr, L., Rodríguez-Lopez, M.A., Mignucci-Giannoni, A.A., Powell, J.A. and Santos, F.R. (2006) Phylogeography, phylogeny and hybridisation in Trichechid sirenians: implications for manatee conservation. Molecular Ecology 15, 433–447.Google Scholar
Zornetzer, H.R. and Duffield, D.A. (2003) Captive-born bottlenose dolphin × common dolphin (Tursiops truncatus × Delphinus capensis). Canadian Journal of Zoology 81, 1755–1762.Google Scholar