Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-20T02:27:13.358Z Has data issue: false hasContentIssue false
  • English
  • Français

Settlement of cyprid larvae of Balanus balanoides and Elminius modestus induced by extracts of adult barnacles and other marine animals

Published online by Cambridge University Press:  11 May 2009

V. N. Larman  and
P. A. Gabbott
V. N. Larman
Affiliation:
N.E.R.C. Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey
P. A. Gabbott
Affiliation:
N.E.R.C. Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey
Get access Rights & Permissions [Opens in a new window]

Extract

Crisp & Meadows (1962, 1963) have shown that aqueous extracts of whole barnacles, when applied to an inert test surface renders the surface attractive to barnacle cyprids thus simulating the gregarious response displayed at settlement. The factor responsible for promoting the settlement of cyprids of Balanus balanoides has been shown to be nondialysable, resistant to boiling in aqueous solution, and can be fractionated by precipitation with ammonium sulphate (Crisp & Meadows, 1962, 1963; Gabbott & Larman, 1971). In their 1962 paper, Crisp & Meadows showed that the settlement factor was present in untreated extracts of all the arthropod groups they examined (including Carcinus maenas) and in extracts of two species of sponges and the fish Blennius pholis. Extracts of other animals and plants were not active. In particular, untreated extracts of the bivalves Mytilus edulis and Ostrea edulis were inactive when assayed against cyprids of B. balanoides. Recently one of us has shown that boiled extracts, partially purified by precipitation with ammonium sulphate, of whole barnacles (B. balanoides, Elminius modestus and B. hameri), crab carapace (Carcinus maenas), whole heads of the blenny (Blennius pholis) and of the body tissues (excluding the shell) of O. edulis and M. edulis, all contain a characteristic group of acidic proteins, or protein-carbohydrate complexes, with iso-electric points in the range pH 4.0–6.0 (Larman, 1975). This finding has prompted us to re-examine the specificity of the settlement response in barnacles.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 55 , Issue 1 , March 1975 , pp. 183 - 190
Copyright
Copyright © Marine Biological Association of the United Kingdom 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bayne, B. L., 1969. The gregarious behaviour of the larvae of Ostrea edulis L. at settlement. Journal of the Marine Biological Association of the United Kingdom, 49, 327–56.CrossRefGoogle Scholar
Cole, H. A. & Knight-Jones, E. W., 1949. The setting behaviour of larvae of the European flat oyster Ostrea edulis L., and its influence on methods of cultivation and spat collection. Fishery Investigations, Ministry of Agriculture, Fisheries and Food, Ser. 2, 17, No. 3, 1–39.Google Scholar
Crisp, D. J., 1967. Chemical factors inducing settlement in Crassostrea virginica (Gmelin). Journal of Animal Ecology, 36, 329–35.CrossRefGoogle Scholar
Crisp, D. J. & Meadows, P. S., 1962. The chemical basis of gregariousness in cirripedes. Proceedings of the Royal Society, B, 156, 500–20.Google Scholar
Crisp, D. J. & Meadows, P. S., 1963. Adsorbed layers: the stimulus to settlement in barnacles. Proceedings of the Royal Society, B, 158, 364–87.Google Scholar
Gabbott, P. A. & Larman, V. N., 1971. Electrophonetic examination of partially purified extracts of Balanus balanoides containing a settlement inducing factor. In: Proceedings 4th European Marine Biological Symposium, ed. Crisp, D. J., 143–53. Cambridge University Press.Google Scholar
Groves, W. E., Davis, F. C. Jr., & Sells, B. H., 1968. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Analytical Biochemistry, 22, 195–210.CrossRefGoogle ScholarPubMed
Hunt, S., 1970. Polysaccharide-protein complexes in invertebrates. London and New York: Academic Press.Google Scholar
Knight-Jones, E. W., 1953. Laboratory experiments on gregariousness during setting in Balanus balanoides and other barnacles. Journal of Experimental Biology, 30, 584–98.CrossRefGoogle Scholar
Knight-Jones, E. W., 1955. The gregarious setting reaction of barnacles as a measure of systematic affinity. Nature, London, 174, 266.CrossRefGoogle Scholar
Larman, V. N., 1975. In preparation.Google Scholar
Meadows, P. S. & Campbell, J. I., 1972. Habitat selection by aquatic invertebrates. In: Advances in Marine Biology, eds. F.S., Russell & M., Yonge, 271–382. London and New York: Academic Press.Google Scholar
Veitch, F. P. & Hidu, H., 1971. Gregarious setting in the American oyster Crassostrea virginica Gmelin. I. Properties of a partially purified ‘setting factor’. Chesapeake Science, 12, 173–8.CrossRefGoogle Scholar
Wilson, D. P., 1970. Additional observations on larval growth and settlement of Sabellaria alveolata. Journal of the Marine Biological Association of the United Kingdom, 50, 1–31.CrossRefGoogle Scholar